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http://dx.doi.org/10.20307/nps.2020.26.3.200

Effects of Compounds from Physalis angulata on Fatty Acid Synthesis and Glucose Metabolism in HepG2 Cells via the AMP-activated Protein Kinase Pathway  

Hoa, Hoang Thai (National Institute of Medical Materials)
Thu, Nguyen Thi (National Institute of Medical Materials)
Dong, Nguyen Thuong (National Institute of Medical Materials)
Oanh, Tran Thi (Institute for Research and Development of Organic Products)
Hien, Tran Thi (Institute of Laboratory Medicine, Lund University)
Ha, Do Thi (National Institute of Medical Materials)
Publication Information
Natural Product Sciences / v.26, no.3, 2020 , pp. 200-206 More about this Journal
Abstract
The ability of the total extract from Physalis angulata; three fractions after partitioning with n-hexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 - 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 ㎍/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 - 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC. The effects of compounds 2 and 4 (10 μM) on FAS and SREBP-1c expression under high glucose conditions (30 mM) in HepG2 cells were evaluated further. Both dose-dependently inhibited FAS and SREBP-1c expression as well as lipid accumulation (1 - 10 μM) were compared to high-concentration glucose control, which upregulated FAS and SREBP-1c. These results suggest that compounds 2 and 4 upregulate AMPK, suppress FAS and SREBP-1c, and have potential effects on glucose and lipid metabolism.
Keywords
Physalis angulata; Withanolides; AMPK; ACC; FAS; SREBP-1c;
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