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Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice (형질전환 벼에서 brazzein 감미단백질의 안정적인 발현)

  • Lee, Ye Rim;Akter, Shahina;Lee, In Hye;Jung, Yeo Jin;Park, So Young;Cho, Yong-Gu;Kang, Kwon Kyoo;Jung, Yu Jin
    • Journal of Plant Biotechnology
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    • v.45 no.1
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    • pp.63-70
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    • 2018
  • Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.

Life Satisfaction of Residents in Regional Community and Affecting Health Related Variables (지역사회주민의 생활 만족도와 이에 영향을 미치는 건강관련요인 연구)

  • Koo, Hyun-Jin;Nam, Chul-Hyun;Chung, Ho-Yoon;Ha, Soon-Hee;Bae, Hyun-Sook;Jung, Hyun-Suk;Lee, Wha-Soo
    • Journal of Society of Preventive Korean Medicine
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    • v.12 no.2
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    • pp.101-114
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    • 2008
  • The current study was conducted to produce basic data for the policy establishment to improve the life satisfaction of residents in regional communities. The study subjected total 1,400 residents living in youngnam province in our nation for the survey by using convenience sampling. The survey took about 4 months started from March 1, 2007 to June 30, 2007. The abstract and conclusion of the result could be summarized as in below. 1. In subjective health status, 45.4% responded as normal, and the health status scored $2.68{\pm}0.67$ in 5 point scale(53.6 in 100 point scale). 2. The level of life satisfaction was found to show the score of $19.57{\pm}3.39$ in 30 point scale(65.2 in 100 point scale). Characteristically, the highest score was found in male in both age groups younger than 19 and in 20s, but lowest score was found in the age group in 40s(p<0.001). In the level of life satisfaction, 29.6% responded as satisfactory and 11.9% responded as not satisfactory. 3. In reviewing the life satisfaction by the health related characteristics, 65.7% of whom responded as very healthy answered satisfactory in life, and the life satisfaction was significantly high in peoples who do not smoke. However, the level was significantly higher in alcohol drinker than no alcohol drinker. People who have enough sleep tended to satisfy in their life, and the life satisfaction level of whom have leisure turned out to show superiorly high satisfaction level. 4. As the variables affecting on the life satisfaction, sex, age, education level, marital status, health status, stress level, life satisfaction level, healthy life activities and depression level were found, and the influence of these variables was 19.6%. As the above results demonstrate, the life satisfaction of residents in regional community was proportional to health status and highly affected by daily health life. So, the more attentions have to be made on health management and the self effort should not be neglected. Especially, since stress, depression level and leisure life did have effects on life satisfaction, more attentions have to be made on stress solving and on the development of leisure life programs. For the goal, the governments, related private organizations and specialized workforces have to make integrated efforts along with the health improvement project in large perspectives.

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A Study on Dietary Behavior and Health Condition of Employees at Department Stores (백화점 종사자의 식행동과 건강상태에 관한 조사)

  • Kim, Hye-Kyung;Kim, Jin-Hee;Park, Young-Sook
    • Korean Journal of Community Nutrition
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    • v.13 no.3
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    • pp.374-385
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    • 2008
  • A study has been performed to provide the basic information about the current dietary habits, health related behaviors, and body indices and to bring forward the importance of this information to the people's attention based upon the relation between employee's life patterns and health conditions in their daily lives. Three hundred and five department store workers were examined from August 2006 to September 2006. With the average BMI values $23.9{\pm}2.2$ for males and $20.0{\pm}1.9$ for females, both gender groups were in normal, but 62.2% of the males were overweight and 15.4% of the females were under-weight. Regarding dietary and health related factors on how they perceive themselves, as normal were 204 (66.9%) the most and bad and very bad were respectively 43 (14.1%) and 5 (1.6%). Half of the subjects (43.2%) perceived sleeping hours to be insufficient, and 64.4% of them need to exercise regularly. As problems related to eating habits, they reported irregular meal times, overeating, preference of hot and spicy food, skipping meal, unbalanced meals. Regarding weight control they have attempted were the most (73.0%), after weight reduction, 51.2% of the subjects had side effects, such as gastrointestinal troubles, anemia, dizziness, sense of fatigue, constipation, physiological disorder, and diarrhea, etc. In the food habit score, it was shown that overall average score of the subjects was $62.63{\pm}9.86$ which is lower than other studies. Female ($62.76{\pm}10.15$) had better score than male ($61.67{\pm}8.06$). While the item with the highest point was eat all three meals of the day, that was the lowest point, exercise every day. The food habit score of the younger group had lower than older group, and also they preferred sweet foods to other group. The results suggest that nutrition education for workers at specific working fields needs to be more focused on the improvement of dietary habits and health status of workers.

A Study on Issues and Improvements Plan for National Safety Assessment (국가안전대진단의 문제점과 개선방안)

  • Shin, Jae-Hun;Kim, Sang-Woon
    • Journal of the Society of Disaster Information
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    • v.14 no.3
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    • pp.334-342
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    • 2018
  • Purpose: The purpose of this study is to examine and improve the issues related to National Safety Inspection currently operation and reform the system for response to various disasters. Method: For the completion, this study established theoretical background using materials related to National Safety Inspection and identified the issues using the open statistical data for National Safety Inspection to suggest the improvement plans. Results: National Safety Inspection has been operating a complete large-scale inspection on risk factors in cooperation with the central government, local governments, and citizens since 2015 to remove various disaster factors neglected in people's daily lives. Operation of National Safety Inspection had positive influence in preventing major disasters. However, inefficient activity is pointed out due to issues related to cooperation with other institutes, limitation for citizen's positional role, and superficial inspection. Conclution: To solve this issue, this study proposed strengthening of communication activity with disaster institutes, strengthening of efficiency through citizen participation by type, and actualization of checklist used in National Safety Inspection.

A study on the effect of sensemaking of the chief executives of the social welfare organizations on their leadership development - Centered on the Community Care Centers for the Disabled - (사회복지기관장의 센스메이킹이 리더십 발현에 미치는 영향에 관한 연구 - 장애인복지관을 중심으로-)

  • Lee, Eun Kyung
    • Korean Journal of Social Welfare Studies
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    • v.44 no.3
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    • pp.207-236
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    • 2013
  • This article aims to understand the process of effective leadership in the Community Care Centers for the Disabled(CCCD). Using a qualitative approach and the concept of 'Sensemaking', a term introduced by Weick (1995), I described and explored the process of leadership. Sensemaking enables leaders to have a better grasp of what is going on in their environments. Sensemaking theory offers a perspective on leadership that resolves successfully complex situations surrounding the CCCD. The CCCDs face the environmental changes such as a paradigm shift of disability and a rivalry system with private services. In this time of uncertain change, the CCCDs are in need of leadership of the executives who could reduce complexity with shared meaning. Data were collected over 5 weeks from the 6 CCCDs, through semi-structured interviews, with practitioners, middle managers and chief executives. The interview scripts were thematically analyzed through Atlas-ti programme. The findings showed three subjects, the people's perception of environment, organizational visioning and interactions among post positions. Even though the 6 CCCDs were under the same environment, the perceptions and the enactments of the practitioners were influenced by the chief executives' sensemaking. The important factors of the chief executives' sensemaking were the daily interaction as well as ongoing reflection on their experiences.

Study on the Roles and Protection of Seafarers as Essential Workers in the Shipping Industry to Prepare for the Post COVID-19 (포스트 코로나 19 시대를 준비하는 해운산업분야 필수업무종사자로서 선원의 역할과 보호에 대한 연구)

  • Lee, Jin-Woo;Hong, Sung-Hwa;Lee, Jeong-Min;Lee, Chang-Hee
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.2
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    • pp.324-333
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    • 2022
  • The COVID-19 pandemic reminded us again of the importance of essential workers who perform their roles in maintaining the stability of people's daily lives worldwide. Therefore, the International Maritime Organization and overseas countries designated seafarers as essential workers and proposed relevant measures for their protection to enable them to perform their role. In particular, the International Maritime Organization (IMO) urges related associations and member states to support safe boarding and disembarkation of seafarers. However, Seafarers were restricted from boarding or disembarking, shore leave and hospital treatment in certain countries owing to extreme nationalism. This study compares and analyzes the cases of international organizations and major overseas countries to provide a logical basis for the protection of seafarers who play an essential role in the safety of the global supply chain despite the COVID-19 pandemic. This study identifies the need to protect seafarers and their roles as essential workers in the shipping industry and suggests necessary aspects and institutional improvement measures for improving seafarers' working environment to strengthen the shipping industry's resilience. This study is expected to serve as future basic data for national policies aimed at reflecting the roles of seafarers as essential workers and the need to protect them.

A Study on the Analysis of the Congestion Level of Tourist Sites and Visitors Characteristics Using SNS Data (SNS 데이터를 활용한 관광지 혼잡도 및 방문자 특성 분석에 관한 연구)

  • Lee, Sang Hoon;Kim, Su-Yeon
    • Journal of Korea Society of Industrial Information Systems
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    • v.27 no.5
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    • pp.13-24
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    • 2022
  • SNS has become a very close service to our daily life. As marketing is done through SNS, places often called hot places are created, and users are flocking to these places. However, it is often crowded with a large number of people in a short period of time, resulting in a negative experience for both visitors and service providers. In order to improve this problem, it is necessary to recognize the congestion level, but the method to determine the congestion level in a specific area at an individual level is very limited. Therefore, in this study, we tried to propose a system that can identify the congestion level information and the characteristics of visitors to a specific tourist destination by using the data on the SNS. For this purpose, posting data uploaded by users and image analysis were used, and the performance of the proposed system was verified using the Naver DataLab system. As a result of comparative verification by selecting three places by type of tourist destination, the results calculated in this study and the congestion level provided by DataLab were found to be similar. In particular, this study is meaningful in that it provides a degree of congestion based on real data of users that is not dependent on a specific company or service.

Exploring life in a public welfare housing for older adults using Stake's case study methodology (Stake식 질적 사례연구 방법을 통해 살펴본 어느 공공 노인복지주택에서의 삶에 관한 연구)

  • Kim, Yujin
    • 한국노년학
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    • v.40 no.1
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    • pp.51-72
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    • 2020
  • The purpose of this study is to understand the experiences of older adults living in the public welfare housing for the elderly and to examine what kind of this housing is as the housing alternative. Through the Robert Stake's intrinsic case study, it explored how older residents experienced this place, and how the relationships between residents, office workers, and community had been in the process, and what kind of issues were there. Various kinds of data, such as resident surveys, participant observations, interviews with 76 elderly residents and 4 office workers, and related newspaper articles, were analyzed using the thematic analysis method and methods suggested by Stake(1995). The results are presented as follows. First, it looked at who lived here and explored what was happening in this place where people of various backgrounds were gathered. Second, four types of life could be seen centering on the social relationship and daily life of older adults. Third, this study examined how the various ways of life were related to the main reasons for moving in here, and summarized how the residents perceived this place. The fourth and the fifth examined the relationships and dynamics among older residents, with office workers, and with the community, focusing on the two issues most commonly mentioned by residents. The results of this study show that, as a living organism, the interaction between the systems affects not only the culture of the housing, but also the lives of older adults. In particular, it can be seen how the perceptions of the practitioners are affecting the lives of the elderly. Based on the results of the study, further considerations were discussed.

Hotel employee's perceptions of artificial intelligence concierge robots effect on switching cost, resistance, turnover intention (호텔 종업원의 인공지능 컨시어지로봇에 대한 인식이 전환비용, 저항 및 이직의도에 미치는 영향)

  • Wang, Danping;Chung, Namho
    • Journal of Service Research and Studies
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    • v.13 no.4
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    • pp.206-223
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    • 2023
  • The introduction of Smart technologies such as Artificial Intelligence(AI) systems are have a powerful impact in a variety of industry fields. Some experts predict that smart technology will completely change people's daily life and work styles, causing technological innovation, productivity improvement, and discovery and emergence of new fields. On the one hand, this vision cannot ignore negative views and concerns. Despite many social debates about employment, such as job loss and rising unemployment, there have not been many studies based on employee experience that provide a fundamental solution to the conflict between AI and employment. Therefore, this study finds out the effects and related factors of AI concierge robots for hotel employees, focusing on the hotel industry, and how employees' perceptions of AI concierge robots affect user resistance and turnover intention. This study, conducted a questionnaire survey of 322 hotel employees who had experience working with AI concierge robots in China, and used SPSS and SmartPLS statistical analysis programs to draw conclusions. We found that hotel employees' perceptions of AI concierge robots were significantly related to user resistance and turnover intention, and this association was related to employee self-efficacy, perceived organizational support, quality of AI services and new tasks. In addition, it was found that the quality of AI concierge robots directly or indirectly had the greatest influence on user resistance and turnover intention. The findings of this study provide theoretical implications for academia and practical implications for industry practitioners.

Comparative study on the role of the public sector in the health care system -Comparison of the United States and Korea in social risk situations- (의료보장 체계에서의 공공 부분의 역할 비교연구 -사회적 위험 상황 속의 미국과 한국의 비교-)

  • Kim Jong Hwi;Hyun-Seung Park
    • Industry Promotion Research
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    • v.9 no.2
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    • pp.95-102
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    • 2024
  • This study aims to compare the role of the public sector in the U.S. and Korean medical security systems and study response measures in the social risk situation of the COVID-19 virus. The COVID-19 pandemic was a typical case of a 'disaster' that spread across the world across borders in a short period of time and caused serious social welfare losses by increasing the annual number of deaths by approximately 4% in 2020. Threats to health security, such as changes in social order, unpredictable endings, prolonged control of daily life, and deepening inequality, affected the economy, politics, and environment as a whole, and people had to experience anxiety and confusion due to mental and physical stress. Furthermore, developed countries failed to provide help to low-income countries in the face of global disasters. In this situation, the country's disaster management capacity to minimize harm and secure resilience, especially disaster response capacity in the health and medical field, is inevitably very important. Therefore, this study compares how the health insurance system, which is a system to guarantee citizens' right to life, differs from the United States, a liberal health care country, and raises the need to strengthen the role of the public sector.