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http://dx.doi.org/10.5010/JPB.2018.45.1.063

Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice  

Lee, Ye Rim (Department of Horticultural Life Science, Hankyong National University)
Akter, Shahina (Department of Horticultural Life Science, Hankyong National University)
Lee, In Hye (Biological Resources Division, National Institute of Biological Resources)
Jung, Yeo Jin (Department of Horticultural Life Science, Hankyong National University)
Park, So Young (Department of Horticultural Science, Chungbuk National University)
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Kang, Kwon Kyoo (Department of Horticultural Life Science, Hankyong National University)
Jung, Yu Jin (Department of Horticultural Life Science, Hankyong National University)
Publication Information
Journal of Plant Biotechnology / v.45, no.1, 2018 , pp. 63-70 More about this Journal
Abstract
Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.
Keywords
Brazzein; Overexpression; Sweetener; Transgenic rice;
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Times Cited By KSCI : 4  (Citation Analysis)
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