• International Journal of Fuzzy Logic and Intelligent Systems
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• v.7 no.1
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• pp.49-57
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• 2007

Using the idea of degree of openness and degree of nonopenness, Coker and Demirci [5] defined intuitionistic fuzzy topological spaces in Sostak's sense as a generalization of smooth topological spaces and intuitionistic fuzzy topological spaces. M. N. Mukherjee and S. P. Sinha [10] introduced the concept of fuzzy irresolute maps on Chang's fuzzy topological spaces. In this paper, we introduce the concepts of fuzzy (r,s)-irresolute, fuzzy (r,s)-presemiopen, fuzzy almost (r,s)-open, and fuzzy weakly (r,s)-continuous maps on intuitionistic fuzzy topological spaces in Sostak's sense. Using the notions of fuzzy (r,s)-neighborhoods and fuzzy (r,s)-semineighborhoods of a given intuitionistic fuzzy points, characterizations of fuzzy (r,s)-irresolute maps are displayed. The relations among fuzzy (r,s)-irresolute maps, fuzzy (r,s)-continuous maps, fuzzy almost (r,s)-continuous maps, and fuzzy weakly (r,s)-cotinuous maps are discussed.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.12
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• pp.4937-4944
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• 2015

MicroRNAs (miRNAs) are emerging as critical regulators in carcinogenesis and tumor progression. Recently, miR-99a has been reported as a tumor suppressor gene in various human cancers, but its functions in the context of anaplastic thyroid cancer (ATC) remain unknown. In this study, we reported that miR-99a was commonly downregulated in ATC tissue specimens and cell lines with important functional consequences. Overexpression of miR-99a not only dramatically reduced ATC cell viability by inducing cell apoptosis and accumulation of cells at G1 phase, but also inhibited tumorigenicity in vivo. We then screened and identified a novel miR-99a target, mammalian target of rapamycin (mTOR), and it was further confirmed by luciferase assay. Up-regulation of miR-99a would markedly reduce the expression of mTOR and its downstream phosphorylated proteins (p-4E-BP1 and p-S6K1). Similar to restoring miR-99a expression, mTOR down-regulation suppressed cell viability and increased cell apoptosis, whereas restoration of mTOR expression significantly reversed the miR-99a antitumor activity and the inhibition of mTOR/p-4E-BP1/p-S6K1 signal pathway profile. In clinical specimens and cell lines, mTOR was commonly overexpressed and its protein levels were statistically inversely correlated with miR-99a expression. Taken together, our results demonstrated for the first time that miR-99a functions as a tumor suppressor and plays an important role in inhibiting the tumorigenesis through targeting the mTOR/p-4E-BP1/p-S6K1 pathway in ATC cells. Given these, miR-99a may serve as a novel prognostic/diagnostic and therapeutic target for treating ATC.

• Nutrition Research and Practice
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• v.14 no.1
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• pp.25-31
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• 2020

BACKGROUND/OBJECTIVES: To date, sodium intake has been evaluated based on spot urine instead of 24-hour (hr) urine collection. Nevertheless, the optimal method for assessing daily sodium intake remains unclear. SUBJECTS/METHODS: Fifteen male (age 32.7 ± 6.5 years) participants were offered 3 meals with a total of 9-10 g salt over 24 hours, and 24-hr urine was collected from the second-void urine of the first day to the first-void urine of the second day. Twenty-four-hr urinary sodium (24UNa) was estimated using Tanaka's equation and the Korean formula, and spot urine Na, potassium (K), chloride (Cl), urea nitrogen (UN), creatinine (Cr), specific gravity (SG) and osmolality (Osm) were measured. The ratios of urinary Na to other parameters were calculated, and correlations with total measured 24UNa were identified. RESULTS: Average 24-hr urine volume was 1,403 ± 475 mL, and measured 24UNa was 143.9 ± 42.1 mEq (range, 87.1-239.4 mEq). Measured 24UNa was significantly correlated with urinary Na/UN (r = 0.560, P < 0.01), urinary Na/Osm (r = 0.510, P < 0.01), urinary Na/Cr (r = 0.392, P < 0.01), urinary Na/K (r = 0.290, P < 0.01), 24UNa estimated using Tanaka's equation (r = 0.452, P < 0.01) and the Korean formula (r = 0.414, P < 0.01), age (r = 0.548, P < 0.01), weight (r = 0.497, P < 0.01), and height (r = 0.393, P < 0.01) in all spot urine samples. Estimated 24UNa based on the second-void spot urine of the first day tended to be more closely correlated with measured 24UNa than were estimates from the other spot urine samples. The significant parameters correlated with the second-void urine of the first day were urinary Na/K (r = 0.647, P < 0.01), urinary Na/Cr (r = 0.558, P < 0.05), and estimated 24UNa using Tanaka's equation (r = 0.616, P < 0.05) and the Korean formula (r = 0.588, P < 0.05). CONCLUSIONS: Second-void urine is more reliable than first-void urine for estimating 24UNa. Urinary Na/K in the second-void urine on the first day is significantly correlated with 24UNa. Further studies are needed to establish the most reliable index and the optimal time of urine sampling for predicting 24UNa.

• Korean Journal of Microbiology
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• v.48 no.4
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• pp.319-324
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• 2012

In this study, we investigated molecular divergences and phylogenetic characteristics of the 16S ribosomal RNA (rRNA) and RNA polymerase beta subunit (rpoB) gene sequences from the order Oscillatoriales (Cyanobacteria). The rpoB of Oscillatoriales showed higher genetic divergence when compared with those of 16S rRNA (p-distance: rpoB=0.270, 16S=0.109), and these differences were statistically significant (Student t-test, p<0.001). Phylogenetic trees of 16S rRNA and rpoB were generally compatible; however, rpoB tree clearly separated the compared Oscillatoriales taxa, with higher phylogenetic resolution. In addition, parsimony analyses showed that rpoB gene evolved 2.40-fold faster than 16S rRNA. These results suggest that the rpoB is a useful gene for the molecular phylogenetics and species discrimination in the order Oscillatoriales.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1100-1105
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• 2005

Previous work has identified a Streptomyces coelicolor gene, rns, encoding a 140 kDa protein (RNase ES) that exhibits the endoribonucleolytic cleavage specificity characteristic of RNase E and confers viability on and allows the propagation of E. coli cells lacking RNase E. Here, we identify a putative S. coelicolor 9S rRNA sequence and sites cleaved by RNase ES. The cleavage of the S. coelicolor 9S rRNA transcript by RNase ES resulted in a 5S rRNA precursor (p5S) that had four and two additional nucleotides at the 5' end and 3' ends of the mature 5S rRNA, respectively. However, despite the similarities between RNase E and RNase ES, these enzymes could accurately process 9S rRNA from just their own bacteria, indicating that these ancient enzymes and the rRNA segments that they attack appear to have co-evolved.

• Kyungpook Mathematical Journal
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• v.55 no.4
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• pp.909-931
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• 2015

This paper presents some new paranormed sequence spaces $X(r,s,t,p;{\Delta})$ where $X{\in}\{l_{\infty}(p),c(p),c_0(p),l(p)\}$ defined by using generalized means and difference operator. It is shown that these are complete linear metric spaces under suitable paranorms. Furthermore, the ${\alpha}$-, ${\beta}$-, ${\gamma}$-duals of these sequence spaces are computed and also obtained necessary and sufficient conditions for some matrix transformations from $X(r,s,t,p;{\Delta})$ to X. Finally, it is proved that the sequence space $l(r,s,t,p;{\Delta})$ is rotund when $p_n$ > 1 for all n and has the Kadec-Klee property.

• The Journal of the Korea Contents Association
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• v.22 no.7
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• pp.432-442
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• 2022

This study aimed to investigate depression, fatigue, and role-overload in nurses caring for COVID-19 patients and identify factors affecting depression. Data were collected from 142 nurses caring for COVID-19 patients at four designated COVID-19 hospitals using self-report questionnaires, from September 8, 2020, to September 17, 2020. The subjects' scores for depression, fatigue, and role-overload were 14.51±9.54, 3.19±0.64, and 3.30±0.64 points, respectively. Depression was positively correlated with fatigue (r=.40, p<.001) and role-overload (r=.30, p<.001), and fatigue was positively correlated with role-overload (r=.54, p<.001). Factors affecting depression included fatigue (β=.23, p=.014), health status (moderate: β=.28, p=.001; unhealthy: β=.26, p=.002), and hospital type (university hospital: β=.18, p=.024). The explanatory power (R²) of the model was 28.1%. In order to manage the nurse's depression level, a strategy for lowering the fatigue level and a strategy for improving the nurse's health statue are needed.

• Bulletin of the Korean Chemical Society
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• v.18 no.3
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• pp.305-310
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• 1997

The synthesis and characterization of the platinum heterocyclic carboxaldehyde thiosemicarbazone complexes [NC5H4CRNNC(S)NHR'PtCl] (R=H, R'=CH3(1); R=CH3, R'=CH3(2); R=CH3, R=H(3)) and diphenylphosphinophenyl carboxaldehyde thiosemicarbazone complexes [Ph2PC6H4CHNNC(S)NHRPtCl] (R=CH3(5); R=iC3H7(6); R=Ph(7)) are described. Compounds 1-3 were prepared by reaction of Pt(SEt2)2Cl2 with 2-acetylpyridine-4-alkylthiosemicarbazone in the presence of NEt3. Compounds 5-7 were prepared using Pt(SEt2)2Cl2 in toluene with diphenylphosphinophenyl carboxaldehyde alkylthiosemicarbazone. The compounds have been characterized by microanalysis, NMR (1H, 13C, 31P) spectroscopy, and single-crystal X-ray diffraction. X-ray single crystal diffraction analysis reveals that compound 5 is a mononuclear platinum compound with P,N,S-coordination mode.

• Journal of the Korea Convergence Society
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• v.11 no.9
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• pp.433-441
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• 2020

This study was conducted to identify the factors that influence the other behaviors taken by nursing students during online lectures. The study subjects were 304 nursing students in three universities. Data were collected between April 20 and 30, 2020, using by completing structured self report questionnaires. Data were analyzed using T-test, ANOVA, Pearson's correlation coefficient, and multiple regression using SPSS 26.0 program. In correlation analysis, significant negative correlations were found between other behaviors, interest(r=-17, p<.01), understanding(r=-19, p<.01), needs(r=-12, p<.05), learning motivation(r=-12, p<.05), self-regulation efficacy(r=-11, p<.05), learning confidence(r=-14, p<.05), lecture satisfaction(r=-22, p<.01), lecture flow(r=-24, p<.01). In the multiple regression analysis, learning confidence, prefer to discuss & present (β=.19), lecture flow(β=-.15), lecture satisfaction(β=-.15) were statistically significant factors that explained 10.6% of variance of other behaviors taken by nursing students during online lectures. Thus, we suggest to develop that teaching methods and programs to reduce other behaviors taken by nursing students during online lectures.

• The Plant Pathology Journal
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• v.28 no.3
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• pp.295-298
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• 2012

Genetic diversity among Pseudomonas syringae pv. morsprunorum isolates from Prunus mume in Korea and Japan was investigated by comparative sequence analysis of the 16S rRNA gene. The strains included 24 field isolates recovered from P. mume in Korea along with seven Japanese strains. Two strains isolated from P. salicina in Japan, one strain from P. avium in the United Kingdom, and the pathotype strain were also used for comparison with their 16S rRNA gene sequences. Nearly complete 16S rRNA gene sequences were sequenced in all 35 strains, and three sequence types, designated types I, II and III, were identified. Eleven strains consisting of five Korean isolates, five Japanese strains, and one strain from the United Kingdom belonged to type I, whereas the pathotype strain and another 19 Korean isolates belonged to type III. Another four Japanese strains belonged to type II. Type I showed 98.9% sequence homology with type III. Type I and II had only two heterogeneous bases. The 16S rRNA sequence types were correlated with the races of P. syringae pv. morsprunorum. Type I and II strains belonged to race 1, whereas type III isolates were included in race 2. Sequence analyses of the 16S rRNA gene from P. syringae pv. morsprunorum were useful in identifying the races and can further be used for epidemiological surveillance of this pathogen.