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http://dx.doi.org/10.7845/kjm.2012.057

Molecular Divergences of 16S rRNA and rpoB Gene in Marine Isolates of the Order Oscillatoriales (Cyanobacteria)  

Cheon, Ju-Yong (Department of Green Life Science, Sangmyung University)
Lee, Min-Ah (Department of Green Life Science, Sangmyung University)
Ki, Jang-Seu (Department of Green Life Science, Sangmyung University)
Publication Information
Korean Journal of Microbiology / v.48, no.4, 2012 , pp. 319-324 More about this Journal
Abstract
In this study, we investigated molecular divergences and phylogenetic characteristics of the 16S ribosomal RNA (rRNA) and RNA polymerase beta subunit (rpoB) gene sequences from the order Oscillatoriales (Cyanobacteria). The rpoB of Oscillatoriales showed higher genetic divergence when compared with those of 16S rRNA (p-distance: rpoB=0.270, 16S=0.109), and these differences were statistically significant (Student t-test, p<0.001). Phylogenetic trees of 16S rRNA and rpoB were generally compatible; however, rpoB tree clearly separated the compared Oscillatoriales taxa, with higher phylogenetic resolution. In addition, parsimony analyses showed that rpoB gene evolved 2.40-fold faster than 16S rRNA. These results suggest that the rpoB is a useful gene for the molecular phylogenetics and species discrimination in the order Oscillatoriales.
Keywords
Oscillatoriales; rpoB gene; Cyanobacteria; DNA similarity; genetic distance;
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