• 한국수산과학회지
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• 제56권1호
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• pp.124-132
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• 2023

This study aimed to cross between Korean and Japanese pure lines of Neopyropia strains to establish cross breeding technology and identify a superior variety that harbors the strength of both parents. Four crossing combinations were tried using three methods, resulting in 1,476 single conchocelis colonies. The three co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) markers (EF-1α/Mse I, TOP2/Mse I, car A/ApaL I) were used to distinguish heterozygotic sporophytes and their maternal lines obtained from the inter and intraspecific cross-fertilization within the wild type of Neopyropia strains. Of the 1,476 colonies, 26.9% (218) were heterozygotes obtained from the nuclear CAPS markers. Their maternal line was clearly confirmed using organelle CAPS marker and chimeric thallus was obtained from crossing experiment of Japanese N. yezoensis (♀) and Korean N. yezoensis (♂). The use of CAPS markers improved the efficiency of crossbreeding by quickly screening heterozygotes and maternal lines in the conchocelis phase, which otherwise required pigmentation mutants as genetic markers.

• 한국작물학회지
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• 제44권3호
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• pp.230-235
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• 1999

To study the effects of an urease inhibitor, N-(n-butyl)-thiophosphoric triamide (NBPT), and a nitrification inhibitor, dicyandiamide (DCD), on nitrogen losses and nitrogen use efficiency, urea fertilizer with or without inhibitors and slowrelease fertilizer (synthetic thermoplastic resins coated urea) were applied to direct-seeded flooded rice fields in 1998. In the urea and the urea+DCD treatments, NH$_4$$^{+}$ -N concentrations reached 50 mg N L$^{-1}$ after application. Urea+NBPT and urea+ NBPT+DCD treatments maintained NH$_4$$^{+}$ -N concentrations below 10 mg N L$^{-1}$ in the floodwater, while the slow-release fertilizer application maintained the lowest concentration of NH$_4$$^{+}$ -N in floodwater. The ammonia losses of urea+NBPT and urea+NBPT+DCD treatments were lower than those of urea and urea+DCD treatments during the 30 days after fertilizer application. It was found that N loss due to ammonia volatilization was minimized in the treatments of NBPT with urea and the slow-release fertilizer. The volatile loss of urea+DCD treatment was not significantly different from that of urea surface application. It was found that NBPT delayed urea hydrolysis and then decreased losses due to ammonia volatilization. DCD, a nitrification inhibitor, had no significant effect on ammonia loss under flooded conditions. The slow-release fertilizer application reduced ammonia volatilization loss most effectively. As N0$_3$$^{[-10]}$ -N concentrations in the soil water indicated that leaching losses of N were negligible, DCD was not effective in inhibiting nitrification in the flooded soil. The amount of N in plants was especially low in the slow-release fertilizer treatment during the early growth stage for 15 days after fertilization. The amount of N in the rice plants, however, was higher in the slow-release fertilizer treatment than in other treatments at harvest. Grain yields in the treatments of slow-release fertilizer, urea+NBPT+ DCD and urea+NBPT were significantly higher than those in the treatments of urea and urea+DCD. NBPT treatment with urea and the slow-release fertilizer application were effective in both reducing nitrogen losses and increasing grain yield by improving N use efficiency in direct-seeded flooded rice field.field.

• 한국초지조사료학회지
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• 제24권2호
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• pp.137-144
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• 2004

본 연구는 1998년 9월부터 2000년 12월까지 충남대학교 생명과학대학내 초지시험포장에서 N 시비수준이 하번초형 혼파초지의 건물수량과 사료가치에 미치는 영향을 구명하고자 수행하였다. 공시초지는 Kentucky bluegrass(Newport) 40%+tall ftscue(Reboll Jr.) 20%+perennial ryegrass(Palmer II ) 10%+ redtop (Barricuda) 10% + red fsscue(Salem) 10% + creeping bentgrass(Crenshaw) 10%로 조성된 하번초형 혼파초지에서 수행하였다. N 시비수준은 100, 150 및 200kg/ha의 3처리를 두어 시험하였으며, 얻어진 결과는 다음과 같다. 1. 2년 평균 ha당 건물수량은 N 시비수준이 높아짐에 따라 현저히 증가되어, N 100, 150 및 200kg 시비수준에서 각각 6,551, 8,182 및 9,501kg을 나타내었다(p<0.05). 2. N 시비수준이 높아짐에 따라 CP 함량과 DMD는 증가된 반면에, 섬유소 함량은 감소되었다(p＜0.05). 3. 2년 편균 ha당 CPDM과 DDM 수량은 N 시비수준이 높아짐에 따라 뚜렷이 증가되었다 (p<0.05). 이상의 결과를 종합해 볼 때, 하번초형 혼파초지에서 부수적으로 생산되는 목초의 건물수량과 사료가치를 높이기 위한 N 시비수준은 200kg/ha이 적당할 것으로 판단된다. 그러나, 그 이상의 N 시비는 이용목적과 비용 등을 고려하는 것이 좋을 것으로 사료된다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2009년도 학술발표회 초록집
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• pp.1994-1998
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• 2009

Nutrients were investigated for surface water and ground water of paddy field and upland in a rural watershed from May 2008 to January 2009. The cocentrations of TN and $NO_3-N$ in ground water higher than those in surface water due to fertilization on cabbage upland neighboring a river during May to September, but lower than those in surface water during October to January. However, the concentrations TP and $PO_4-P$ in ground water were lower than those in surface water. TP and $PO_4-P$ concentrations in ground water of paddy field were generally lower than those of upland.

• 한국수산과학회지
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• 제49권5호
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• pp.671-674
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• 2016

We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm³); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2007년도 학술발표회 논문집
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• pp.157-161
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• 2007

Nutrients were investigated for surface water, bottom sediment and ground water in a rural watershed from January 2006 to March 2007. The concentrations of TN and $NO_3-N$ in ground water were higher than those in surface water due to fertilization on cabbage upland neighboring a river during March to August, but lower than those in surface water during September to February. However, the concentrations TP and $PO_4-P$ in ground water were lower than those in surface water. The concentrations of TP and $PO_4-P$ in surface water was lower than those in bottom sediment. The TP concentration in the bottom greatly decreased during rainy season. due to flush sediment of bottom, and then gradually increased.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2001년도 춘계 학술발표논문집
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• pp.179-180
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• 2001

1. 실험목적 : 벼 휴립건답직파재배시 절수재배에 알맞은 질소 수비량을 구명하고자 함. 2. 재료 및 방법 가. 공시품종 : 동진벼 나. 공시토양 : 전북통(미사질양토) 다. 물관리방법 : 3엽기부터 완전낙수기까지 5일 간격 고랑관개 라. 처리내용 -파종량(kg/ha) : 60, 100 -질소 수비량(kg/ha) : 24(50% 감비), 48(표준), 75(50% 증시), 96(100% 증시)(중략)

• 한국동물생명공학회지
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• 제35권1호
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• pp.2-11
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• 2020

Assisted reproductive technologies (ART) merely depend on improving the oocyte maturation and their developmental competence to produce good quality embryos. Oocyte maturation passes through long and complex molecular steps starts from the early embryonic life and ends with sperm fertilization. Oocyte developmental competence can be attained by improving the nuclear and cytoplasmic mechanisms together with some epigenetic maturation. In this review, we highlight the cornerstones of oocyte maturation on both nuclear and cytoplasmic levels. Interfering or supporting these molecular mechanisms would help in the development of novel regulating agents for reproductive performance of humans and livestock species.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.283-283
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• 2004

The eggs of most animal species are surrounded by an extracellular matrix known as chorion, egg envelope, egg coat, or zona pellucida. Development of fish embryo usually takes several days in an aquatic environment. During embryonic development, the chorion must protect embryo from physical damage and microbial infection in the exposed aquatic environment. (omitted)

• 한국토양비료학회지
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• 제26권1호
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• pp.31-36
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• 1993

Sr-90으로 처리(處理)된 토양(土壤)에서 질소(窒素), 인산(燐酸), 석회질(石灰質) 비료(肥料)의 시비조건(施肥條件)에 따른 대두(大豆)의 생육(生育)과 Sr-90 흡수양상(吸收樣相)을 조사하였다. 1. 지상부(地上部) 건물중(乾物重)으로 나타낸 대두수량(大豆收量)은 $NO_3-N$보다 $NH_4-N$에서 다소 높았고, 질소시비량(窒素施肥量)의 증가(增加)에 따라 감소(減少)되었으며, 인산(燐酸)과 석회(石灰) 시비(施肥)는 수량증가(收量增加) 효과가 있었다. 2. 생육시기(生育時期)에 따른 대두(大豆)의 Sr-90 흡수(吸收)는 질소시비(窒素施肥)에 의해 억제(抑制)되었는데 그 효과는 $NO_3-N$보다 $NH_4-N$에서 더 높았다. 3. 대두(大豆)의 Sr-90 흡수(吸收)에 대한 $NO_3-N$와 $NH_4-N$ 증시효과는 인산(燐酸) 혼용시(混用時)에 더욱 억제효과가 있었다. 4. 인산(燐酸)과 석회시비(石灰施肥)는 대두(大豆)의 Sr-90 흡수억제(吸收抑制)에 상당한 효과가 있었고 시험구(試驗區) 중 인산(燐酸)+석회(石灰)+$NH_4-N$구(區)에서 가장 낮은 흡수(吸收)를 보였다.