Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Cytogenetic Analysis of All-Female Triploid Olive Flounder Paralichthys olivaceus for Ploidy Verification

전 암컷 3배체 넙치(Paralichthys olivaceus)에 대한 효율적인 세포유전학 분석법

  • Ko, Min Gyun (Department of Fisheries Biology, Pukyong National University) ;
  • Jung, Hyo Sun (Department of Fisheries Biology, Pukyong National University) ;
  • Lee, Hyo Bin (Department of Fisheries Biology, Pukyong National University) ;
  • Kim, Dong Soo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University)
  • 고민균 (부경대학교 수산생물학과) ;
  • 정효선 (부경대학교 수산생물학과) ;
  • 이효빈 (부경대학교 수산생물학과) ;
  • 김동수 (부경대학교 해양바이오신소재학과)
  • Received : 2016.09.09
  • Accepted : 2016.10.22
  • Published : 2016.10.31
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Abstract

We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm3); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.

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References

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