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http://dx.doi.org/10.5657/KFAS.2016.0671

Cytogenetic Analysis of All-Female Triploid Olive Flounder Paralichthys olivaceus for Ploidy Verification  

Ko, Min Gyun (Department of Fisheries Biology, Pukyong National University)
Jung, Hyo Sun (Department of Fisheries Biology, Pukyong National University)
Lee, Hyo Bin (Department of Fisheries Biology, Pukyong National University)
Kim, Dong Soo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.5, 2016 , pp. 671-674 More about this Journal
Abstract
We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm3); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.
Keywords
KingNupchi; Paralichthys olivaceus; Triploid; Silver staining;
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Times Cited By KSCI : 1  (Citation Analysis)
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