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Purification and Characterization of Six Fibrinolytic Serine-Proteases from Earthworm Lumbricus rubellus

  • Cho, Il-Hwan;Choi, Eui-Sung;Lim, Hun-Gil;Lee, Hyung-Hoan
    • BMB Reports
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    • v.37 no.2
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    • pp.199-205
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    • 2004
  • The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fibrinolytic activities of the six fractions on the fibrin plates ranged from 20.8 to 207.2 unit/mg with rank orders of F6 > F2 > F5 > F3 > F1 > F4. The molecular weights of each iso-enzyme, as estimated by SDS-PAGE, were 24.6 (F1), 26.8 (F2), 28.2 (F3), 25.4 (F4), 33.1 (F5), and 33.0 kDa (F6), respectively. The plasminogen was activated into plasmin by the enzymes. The optimal temperature of the six iso-enzymes was $50^{\circ}C$, and the optimal pH ranged from pH 4-12. The four iso-enzymes (F1-F4) were completely inhibited by PMSF. The two enzymes (F5 and F6) were completely inhibited by aprotinin, TLCK, TPCK, SBTI, LBTI, and leupeptin. The N-terminal amino acid (aa) sequences of the first 20 to 22 residues of each fraction had high homology. All six isoenzymes had identical aa residues 2-3 and 13-15. The N-terminal 21-22 aa sequences of the F2, F3, and F4 isoenzymes were almost the same. The N-terminal aa sequences of F5 and F6 were identical.

ON STABILITY OF A GENERALIZED QUADRATIC FUNCTIONAL EQUATION WITH n-VARIABLES AND m-COMBINATIONS IN QUASI-𝛽-NORMED SPACES

  • Koh, Heejeong;Lee, Yonghoon
    • Journal of the Chungcheong Mathematical Society
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    • v.33 no.3
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    • pp.319-326
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    • 2020
  • In this paper, we establish a general solution of the following functional equation $$mf\({\sum\limits_{k=1}^{n}}x_k\)+{\sum\limits_{t=1}^{m}}f\({\sum\limits_{k=1}^{n-i_t}}x_k-{\sum\limits_{k=n-i_t+1}^{n}}x_k\)=2{\sum\limits_{t=1}^{m}}\(f\({\sum\limits_{k=1}^{n-i_t}}x_k\)+f\({\sum\limits_{k=n-i_t+1}^{n}}x_k\)\)$$ where m, n, t, it ∈ ℕ such that 1 ≤ t ≤ m < n. Also, we study Hyers-Ulam-Rassias stability for the generalized quadratic functional equation with n-variables and m-combinations form in quasi-𝛽-normed spaces and then we investigate its application.

Changes of Chemical Compounds in Compost of Municipal Refuse;3. Changes of C/N Ratios in Compost (폐기물의 퇴비화 과정중 물질 변화;3. C/N 율 변화)

  • Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.8 no.1
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    • pp.55-59
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    • 1989
  • To determine the accurate C/N ratio of compost, biodegradable carbon and nitrogen in compost must be analyzed. In biodegradable nitrogen analyzed by Kjeldahl Method, nitrate and nitrite which are available for microbes can not be detected at all. By means of $F{\"{o}}rster$ Method, nitrate and nitrite can be detected. Therefore, various nitrogens in compost were analyzed by Kjeldahl and $F{\"{o}}rster$ Method to investigate the seasonal changes of various C/N ratios of compost. C/N ratios in compost analyzed by Kjeldahl and $F{\"{o}}rster$ Method were decreased rapidly within 2 weeks after composting, then were not changed to 6 weeks, and thereafter were decreased slowly with the lapse of composting time. C/N ratios analyzed by Kjeldahl Method were higher than those analyzed by $F{\"{o}}rster$ Method. C/N ratios of total carbon/total nitrogen analyzed by Kjeldahl Method were higher(5 as C/N ratio or 36%) than those of biodegradable carbon/biodegradable nitrogen analyzed by $F{\"{o}}rster$ Method. Highly positive correlations were observed among C/N ratios of total carbon/total nitrogen analyzed by Kjeldahl Method total carbon/total nitrogen analyzed by $F{\"{o}}rster$ Method and biodegradable carbon/biodegradable nitrogen analyzed by $F{\"{o}}rster$ Method one another.

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ADDITIVE-QUADRATIC ρ-FUNCTIONAL INEQUALITIES IN FUZZY BANACH SPACES

  • LEE, SUNG JIN;SEO, JEONG PIL
    • The Pure and Applied Mathematics
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    • v.23 no.2
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    • pp.163-179
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    • 2016
  • Let $M_1f(x,y):=\frac{3}{4}f(x+y)-\frac{1}{4}f(-x-y)+\frac{1}{4}(x-y)+\frac{1}{4}f(y-x)-f(x)-f(y)$, $M_2f(x,y):=2f(\frac{x+y}{2})+f(\frac{x-y}{2})+f(\frac{y-x}{2})-f(x)-f(y)$ Using the direct method, we prove the Hyers-Ulam stability of the additive-quadratic ρ-functional inequalities (0.1) $N(M_1f(x,y)-{\rho}M_2f(x,y),t){\geq}\frac{t}{t+{\varphi}(x,y)}$ and (0.2) $N(M_2f(x,y)-{\rho}M_1f(x,y),t){\geq}\frac{t}{t+{\varphi}(x,y)}$ in fuzzy Banach spaces, where ρ is a fixed real number with ρ ≠ 1.

Lower Bound of Message Complexity for Election Alogorithm in a Complete Network with Intermittent Faults (간헐적 고장이 있는 완전 네트워크에서 선거알고리즘을 위한 메시지 복잡도의 낮은 경계)

  • Kim, Seong-Dong
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.11
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    • pp.2855-2861
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    • 1998
  • Election is a fundamental problem in the distributed computing. We consider n nodes in the network with f maximum number of faulty links incident on each node, where $f{\le}{\llcorner}(n-1/2){\lrcorner}$. In general, electing a leader, finding the maximum identifier and constructing a spanning tree belong to the same class in the distributed computing because of the same order of the message complexity. Using a spanning tree, we prove that the lower bound of message complexity for a leader election algorithm in an asynchronous complete network with intermittent link faults is $O(n^3)$.

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ON BOUNDARY REGULARITY OF HOLOMORPHIC CORRESPONDENCES

  • Ourimi, Nabil
    • Journal of the Korean Mathematical Society
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    • v.49 no.1
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    • pp.17-30
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    • 2012
  • Let D be an arbitrary domain in $\mathbb{C}^n$, n > 1, and $M{\subset}{\partial}D$ be an open piece of the boundary. Suppose that M is connected and ${\partial}D$ is smooth real-analytic of finite type (in the sense of D'Angelo) in a neighborhood of $\bar{M}$. Let f : $D{\rightarrow}\mathbb{C}^n$ be a holomorphic correspondence such that the cluster set $cl_f$(M) is contained in a smooth closed real-algebraic hypersurface M' in $\mathbb{C}^n$ of finite type. It is shown that if f extends continuously to some open peace of M, then f extends as a holomorphic correspondence across M. As an application, we prove that any proper holomorphic correspondence from a bounded domain D in $\mathbb{C}^n$ with smooth real-analytic boundary onto a bounded domain D' in $\mathbb{C}^n$ with smooth real-algebraic boundary extends as a holomorphic correspondence to a neighborhood of $\bar{D}$.

On the Estimation of the Empirical Distribution Function for Negatively Associated Processes

  • Kim, Tae-Sung;Lee, Seung-Woo;Ko, Mi-Hwa
    • Communications for Statistical Applications and Methods
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    • v.8 no.1
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    • pp.229-235
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    • 2001
  • Let {X$\_$n/, n$\geq$1] be a stationary sequence of negatively associated random variables with distribution function F(x)=P(X$_1$$\leq$x). The empirical distribution function F$\_$n/(x) based on X$_1$, X$_2$,....., X$\_$n/ is proposed as an estimator for F$\_$n/(x). Strong consistency and asymptotic normality of F$\_$n/(x) are studied. We also apply these ideas to estimation of the survival function.

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Study of Electron Transport Coefficients in $C_{n}F_{2n+2}$(n=1,2,3) Molecular Gas ($C_{n}F_{2n+2}$(n=1,2,3) 분자가스의 전자수송계수 연구)

  • Jeon, Byung-Hoon
    • Proceedings of the KIEE Conference
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    • 2006.07c
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    • pp.1455-1456
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    • 2006
  • 반도체 에칭분야에 많이 이용되고 있는 $CF_4$, $C_{2}F_6$, $C_{3}F_8$가스들의 전자수송계수들을 볼츠만 방정식을 이용하여 해석하고자 한다. 특히 혼합가스를 이용하여 확산방전스위치에서 요구되어지는 특성을 파악하고자 할 때 시뮬레이션에 의한 적절한 혼합비 구현을 위하여 이들 순수가스들이 가지고 있는 전자충돌단면적을 해석하고, 전자이동속도와 부착계수 값을 2항과 다항근사 볼츠만 해석을 통해 $0.1{\sim}300$ Td에 걸친 광범위 표에서 해석하고자 한다.

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SUFFICIENT CONDITIONS AND RADII PROBLEMS FOR A STARLIKE CLASS INVOLVING A DIFFERENTIAL INEQUALITY

  • Swaminathan, Anbhu;Wani, Lateef Ahmad
    • Bulletin of the Korean Mathematical Society
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    • v.57 no.6
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    • pp.1409-1426
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    • 2020
  • Let 𝒜n be the class of analytic functions f(z) of the form f(z) = z + ∑k=n+1 αkzk, n ∈ ℕ defined on the open unit disk 𝔻, and let $${\Omega}_n:=\{f{\in}{\mathcal{A}}_n:\|zf^{\prime}(z)-f(z)\|<{\frac{1}{2}},\;z{\in}{\mathbb{D}}\}$$. In this paper, we make use of differential subordination technique to obtain sufficient conditions for the class Ωn. Writing Ω := Ω1, we obtain inclusion properties of Ω with respect to functions which map 𝔻 onto certain parabolic regions and as a consequence, establish a relation connecting the parabolic starlike class 𝒮P and the uniformly starlike UST. Various radius problems for the class Ω are considered and the sharpness of the radii estimates is obtained analytically besides graphical illustrations.

ON FINITE GROUPS WITH THE SAME ORDER TYPE AS SIMPLE GROUPS F4(q) WITH q EVEN

  • Daneshkhah, Ashraf;Moameri, Fatemeh;Mosaed, Hosein Parvizi
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.4
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    • pp.1031-1038
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    • 2021
  • The main aim of this article is to study quantitative structure of finite simple exceptional groups F4(2n) with n > 1. Here, we prove that the finite simple exceptional groups F4(2n), where 24n + 1 is a prime number with n > 1 a power of 2, can be uniquely determined by their orders and the set of the number of elements with the same order. In conclusion, we give a positive answer to J. G. Thompson's problem for finite simple exceptional groups F4(2n).