• Applied Biological Chemistry
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• v.49 no.2
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• pp.114-124
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• 2006

The objective of this research was to evaluate the ability of water and 80% ethanol extracts from one hundred eight mulberry leaves (Morus alba L.) to influence the inhibitory activity of angiotensin converting enzyme (ACE) and xanthine oxidase (XOase). The total phenol contents were that water extracts of ten species (Kakjayongsan (Morus alba L.), Daejungsun (Morus alba L.) etc.) and 80% ethanol extracts of twenty three species (Waryoung (Morus alba L.), Hasusang (Morus alba L.) etc.) showed more than 15 mg/g. The inhibitory activity on angiotensin converting enzyme (ACE) were that ten species (YamanakkadakKaskke (Morus alba L.), Mijiro (Morus alba L.) etc.) showed 100% inhibition rate both of water extracts and 80% ethanol extracts. The rest, water extracts of thirty four species (Cheongilppong (Morus alba L.) etc.) and 80% ethanol extracts of thirty four species (Wonjukojo (Morus alba L.) etc.) showed inhibitory activity (above 90%) on ACE. Also, to search of xanthine oxidase (XOase) inhibition were that water extracts of five species (Cheongsipjosaeng (Morus alba L.), Suwon 3 (Morus alba L.) etc.) and 80% ethanol extracts of Jeokmok (Morus alba L.) showed inhibitory activity (above 50%) on XOase. This result revealed, strong biological activity in spite of has a little total phenol contents. These water and 80% ethanol extracts from mulberry leaves (Morus alba L.) are expected good candidate for development into anti-hypertentive and anti-gout sources.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.53-54
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• 2001

The chromosome number of Morus bombycis Koidz. and Morus monogolica C.K.Schn. growing wild in the Korea Peninsula is diploid (2n=28) and that of Morus tiliaefolia Makino is hecxaploid (2n=84). The somatic cell division of each species is nomal.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.1
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• pp.23-33
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• 2007

A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.1-8
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• 2001

Nucleotide sequence in internal transcribed spacer (ITS) regions of ribosomal DNA among mulberry varieties (Morus species) were analyzed in order to identify the possibility of classification for the species. The variations in the ITS regions were compared among 9 mulberry varieties and one variety of Cudrania species as an outgroup. ITS 1 region of the varieties ranging from 219 to 220 bp in length was 49-50 bp shorter than ITS 2 region. Of 510 sites in the ITS 1 and 2 regions, 148 sites were potentially variable, of which 52% and 48% sites were distributed in ITS 1 and ITS 2 regions, respectively. By pairwise comparisons on the nucleotide sequences in the ITS 1 and 2 regions among 9 mulberry varieties, they were classified into 5 groups. Divergence values of the sequences, however, were considerably low ranging from 0 to 1.3%. Especially, there was no divergence among Backasipmunja, Chungilppong and Milsungpong and Jungyasang, Ssarigol II and Yulbon, respectively.

• Journal of Sericultural and Entomological Science
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• v.37 no.1
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• pp.1-5
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• 1995

The fertilities of indigenous and cultivated mulberry species(varieties) which were polli nated with Morus nigra L. brought from Turkey were investigated. The percentage of ripening sorosis was very high in indigenous species, Morus tiliaefolia Makino and cultivated variety, Keomseolppong and it was around 50% in both Daeryukppong and Cheongilppong However, the flowers of Morus bombycis Koidz. (BOM5-8) and Yongcheonppong fell after pollination. Looking at the sizes of seeds, they are also various. Generally, it was extremely small in Morus tiliaefolia, but it was medium in Keomseolppong, Cheongilppong and Daeryukppong. The fertilization percentage was generally low with 32-42% in the varieties, but it was comparatively high in Keomseolppong and Morus tiliaefolia. The seed germination percentages of Daeryukppong and Morus tiliaefolia are 88.0% and 23.6%, respectively, but it was below 2% in Cheongilppong and Keomseolppong. The cross success percentages are 14.2% and 8.0% in Daeryukppong and Morus tiliaefolia, respectively, but it was below 0.3% in Cheongilppong and Keomseolppong.

• Korean Journal of Medicinal Crop Science
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• v.13 no.4
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• pp.146-149
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• 2005

This study carried out to quantitatively analyze the content of resveratrol and oxyresveratrol in various parts and varieties of Morus alba L. The seperation of resveratrol and oxyresveratrol was performed using a reverse phase HPLC system. As the result, underground part of Morus alba L. contained higher concentration of resveratrol and oxyresveratrol compared with the other parts. Moreover, among the various Morus alba cortex species, Suwon and Shinil had the highest concentration of resveratrol and oxyresveratrol. Therefore, underground part of Suwon and Shinil species were most effective for extraction of resveratrol and oxyresveratrol.

• Journal of Sericultural and Entomological Science
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• v.39 no.2
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• pp.106-113
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• 1997

Three indigenous mulberry species of Morus tiliaefolia Makino, Morus mongolica C. K. Shneider and Morus bombycis Koidz. were naturally crossed and their fertilities and fruit characteristics were investigated during the years of 1996 and 1997. M. tiliaefolia Makino and M. mongolica C.K. Schneider showed low fertility, but M. bombycis Koidz. was medium in fertility. The fruits of M. tiliaefolia Makino were red-purple and midium in size and high sugar content, but those of M. mongolica C.K. Schneider were light red and small but extremely high sugar content. The total acidity of fruits was 0.73, 0.50 and 0.36-0.94% in M. tiliaefolia Makino, M. mongolica C.K. Schneider and M. bombycis Koidz. respectively. Regarding ripenening stage, M. mongolica C.K. Schneider was early and M. tiliaefolia Makino was medium, while M. bombycis Koidz was early or medium.

• Journal of Sericultural and Entomological Science
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• v.43 no.2
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• pp.88-92
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• 2001

The crossabilities among 3 wild mulberry species and 3 cultivars growing in the Korean Peninsula had been studied. The result of the crossabilities of Moms tiliaefolia with Moms bombycis and 3 cultivars were possible, but with Morus mongolica was impossible. Those of Moms mongolica with Moms bombycis, Morus tiliaefolia and 2 cultivars except Daeryukppong were possible too. And those of Morus bombycis with Moms titiaefolia, Moms mongolica were impossible, but with 3 cultivars were possible moderately.

• Korean Journal of Plant Resources
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• v.26 no.3
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• pp.397-402
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• 2013

Morus Folium (Sang-yeop in Korean) is one of the most important Oriental medicinal plants. In Korea, both M. alba and M. cathayana are regarded as the botanical sources for Morus Folium. In order to discriminate M. alba and M. cathayana from their adulterant, M. tricuspidata, mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 2 region was targeted for molecular analysis with universal primers. DNA polymorphisms, including SNP sites, insertions, and deletions, were detected among these three species sequencing data. Based on these DNA polymorphisms, specific primers were designed for the three species respectively. Multiplex PCR was conducted for molecular authentication of M. alba, M. cathayana, and M. tricuspidata with specific primers. The present results indicate that it is possible to identify Morus Folium from its adulterant using mitochondrial nad7 intron 2 region. The established multiplex-PCR system was proved to be effective for identification of Morus Folium. The results indicate that mitochondrial introns can be used for inter-specific polymorphic study, and the described method can be applied for molecular identification of medicinal materials.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.38 no.3 s.116
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• pp.38-46
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• 2006

This study was carried out to investigate the sheet properties of Morus Hanji, made by different pulping methods, such as alkali, alkali-peroxide and sulfomethylated pulping, and different stocks composed to bast and whole stalk fibers. Two species of Morus, M. alba and M. lhou, were used. Pulp yield of the whole stalk parts, its brightness and opacity were higher than those of bast part. In particular sulfomethylated pulp has higher brightness of $62{\sim}63%$, than other pulps(average brightness $30{\sim}40%$). In mechanical properties, paper made of bast fiber has higher strength than those of the whole stalks. Hanji made from M. lhou, whole stalk, and sulfomethylated pulp gave better sheet formation and higher strength than those of the others. Hanji from M. alba bast fiber and whole stalk pulps were not so good sheet properties.