Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Analysis of ITS Nucleotide Sequences in Ribosomal DNA of Morus Species

뽕나무속 식물의 ITS 영역 염기서열 분석

  • 성규병 (농업과학기술원 잠사곤충부) ;
  • 류근섭 (경북대학교 농과대학) ;
  • 김호락 (농업과학기술원 잠사곤충부) ;
  • 남학우 (농업과학기술원 잠사곤충부) ;
  • 구태원 (농업과학기술원 잠사곤충부)
  • Published : 2001.06.01
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Abstract

Nucleotide sequence in internal transcribed spacer (ITS) regions of ribosomal DNA among mulberry varieties (Morus species) were analyzed in order to identify the possibility of classification for the species. The variations in the ITS regions were compared among 9 mulberry varieties and one variety of Cudrania species as an outgroup. ITS 1 region of the varieties ranging from 219 to 220 bp in length was 49-50 bp shorter than ITS 2 region. Of 510 sites in the ITS 1 and 2 regions, 148 sites were potentially variable, of which 52% and 48% sites were distributed in ITS 1 and ITS 2 regions, respectively. By pairwise comparisons on the nucleotide sequences in the ITS 1 and 2 regions among 9 mulberry varieties, they were classified into 5 groups. Divergence values of the sequences, however, were considerably low ranging from 0 to 1.3%. Especially, there was no divergence among Backasipmunja, Chungilppong and Milsungpong and Jungyasang, Ssarigol II and Yulbon, respectively.

Keywords

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