• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1556-1560
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• 2006

Six breeds of riverine buffalo viz. Murrah, Mehsana, Jaffrabadi, Nagpuri, Nili-Ravi and Bhadawari were characterized using FAO-recommended cattle specific microsatellite markers. Among the total of twenty microsatellite markers screened to explore genomic variability of six buffalo breeds, only ten were polymorphic in nature. Four out of ten polymorphic microsatellite loci were rated as informative. The numbers of alleles detected ranged from 2 to 7, with a mean of $5.5{\pm}0.07$ per microsatellite marker. The most polymorphic marker was BM1818 with a total of 7 alleles present at this locus. One breed specific marker was found in each of Mehsana (BM1818) and Bhadawari (ILSTS030) and four were found in Jaffarabadi (BM1818, ILSTS030, ILSTS054 and ILSTS011). Genetic distance (Ds) between the Mehsana and Bhadawari breed was the maximum (0.29), followed by Murrah and Mehsana (0.27), and Nili-Ravi and Bhadawari (0.26). The lowest Ds was found between the Jaffrabadi and Nagpuri breeds which was only 0.05. The highest divergence time of 1318 years was established between Mehsana and Bhadawari breeds whereas it was found to be lowest (272 years) between the Jaffrabadi and Nagpuri breeds.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.327-332
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• 2016

The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average $F_{is}$ value for the breeds ranged from -0.044 (ETH225) to 0.180 (SPS115), while the estimated average $F_{st}$ value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy.

• 원예과학기술지
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• 제31권5호
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• pp.580-589
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• 2013

국내에서 최근에 유통되고 있는 고추 170품종을 대상으로 microsatellite 마커를 이용하여 DNA 프로파일 데이터베이스를 구축하기 위하여 품종식별력이 높은 분자표지의 선정 및 이를 활용한 품종 식별력 및 유전적 유사도 검정 등에 대한 연구를 수행하였다. 고추 형태적 특성이 다른 11품종을 302개의 microsatellite 마커로 검정하여 24개의 다형성이 높은 마커를 선정한 다음 170품종에 대한 DNA 프로파일 데이터베이스를 구축하였다. 고추 170품종을 24개의 microsatellite 마커로 분석하였을 때 마커당 평균 대립유전자수는 6.83개로 나타났고, 최소 2개부터 15개까지 다양한 분포를 나타내었다. PIC 값의 경우 0.324-0.873 범위에 속하였으며 평균값은 0.673으로 높게 나타났다. Microsatellite 마커의 대립유전자를 이용하여 고추 170품종에 대한 계통도를 작성하였을 때 과실의 형태에 따라 3개의 그룹으로 크게 나누어졌으며 모든 품종이 microsatellite 마커의 유전자형에 의해 식별이 되는 것으로 나타났다. 이 연구결과에 의해 개발된 고추 품종별 DNA 프로파일 데이터베이스는 품종보호출원 품종의 선 DNA 검정을 통한 대조품종 선정, 구별성, 균일성, 안정성의 재확인에 매우 유용하게 이용되어질 수 있어 향후, 품종보호권 강화 등에 크게 기여할 수 있을 것으로 사료된다.

• 원예과학기술지
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• 제32권4호
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• pp.525-534
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• 2014

Microsatellite 표지를 이용하여 국내에서 시판되고 있는 멜론 58품종의 식별과 멜론 육종 계통 '10H08'을 이용하여 $F_1$ 종자 순도를 평가하였다. 412개의 microsatellite 표지 중 다형성 정도가 높은 29개는 품종 그룹 내에서도 다양한 유전 변이를 나타내었으며 분자표지의 유전자형에 의해 모든 품종을 식별할 수 있었다. Microsatellite 표지의 대립유전자를 이용하여 멜론 58품종에 대한 계통도를 작성하였을 때 멜론의 형태적 특성과 일치하면서 2개의 대그룹으로 구분되었다. $F_1$ 종자의 순도 검정에 microsatellite 표지를 활용하기 위하여 29개의 표지를 '10H08' 계통의 양친에 대하여 검정하였을 때 5개의 프라이머가 다형성을 보였으며, 이 중 한 개의 프라이머 'CMGAN12'는 양친간에 뚜렷한 다형성 밴드를 나타내었다. 이 프라이머를 192개의 $F_1$ 종자에 대하여 검정하였을 때 자식주로 보이는 개체가 분석된 종자 내에서 명확하게 구분되었다. 본 연구 결과에서 선정된 멜론 품종 식별용 microsatellite 표지는 멜론 품종의 지문화뿐만 아니라 $F_1$ 종자의 순도 검정이 가능하여 종자회사에서 매우 유용하게 활용될 수 있는 것으로 나타났다.

• Journal of the Korean Data and Information Science Society
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• 제17권4호
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• pp.1041-1051
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• 2006

The effectiveness of genetic markers on Hanwoo traceability systems was applied and evaluated from Korean 33 Hanwoo elite sire families. Five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Eleven major microsatellite markers were selected from allele amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification with experimental condition, the match probability(MP) and the relatedness coefficient(R) were computed.

• Mycobiology
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• 제29권1호
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• pp.43-47
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• 2001

Genetic diversity of 21 Korean Phytophthora capsici isolates was analyzed by using several molecular markers such as random amplified polymorphic DNA(RAPD), M-13, microsatellite and random amplified microsatellite sequences(RAMS). The overall average similarity coefficient among the isolates was 86% based on the combined data obtained by the molecular markers. No molecular markers were found to be associated with hosts or geographic regions. In addition to RAPD, analysis based on repeated sequences such as $(GTG)_5$, M-13 and RAMS could be used to assess population structure of P. capsici.

• 식물분류학회지
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• 제48권2호
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• pp.129-133
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• 2018

Ceratopteris thalictroides is a semi-aquatic fern with a circumtropical distribution. Because this species is designated internationally on the IUCN Red List as requiring at least some concern, Korean populations are of great concern for the species' long-term survival, as they are at the northern limit of the species distribution. To establish an effective conservation strategy for those populations at the genetic level, we used the Mi-Seq platform to develop three sets of 25 polymorphic microsatellite markers for C. thalictroides, which is endangered in Korea. In populations sampled from Busan and Gochang, the number of alleles ranged from 2 to 13 (average of 5.64), and plants presented an expected heterozygosity of 0.000 to 0.860. These markers will be useful for evaluating the genetic status and conserving Korean populations of C. thalictroides more effectively.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 한국약용작물학회 공동춘계학술발표회
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• pp.126-127
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• 2006

• 생명과학회지
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• 제16권3호
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• pp.382-386
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• 2006

고양이의 혈통 등록을 위한 개체식별 및 친자판정을 목적으로 microsatellite DNA다형을 조사한 결과 다음과 같은 성적을 얻었다. 고양이 20두를 대상으로 microsatellite DNA다형의 대립유전자를 조사한 본 연구에서는 관찰된 대립유전자의 수는 $3{\sim}8$개 (평균 5.5개)이며 marker별 대립유전자는 FCA005 142bp (0.3750), FCA026 148 bp (0.5500), FCA075 136 bp (0.5000), FCA105 191 bp (0.4250), FCA224 156 bp (0.7750), FCA229 166 bp (0.6500), FCA240 163 bp (0.3000), FCA293 185 bp (0.5000), FCA453 186 bp (0.5500), FCA651 134 bp (0.6750) 대립유전자가 높은 빈도로 관찰되었다. Expected heterozygosity와 PIC는 각각 $0.390{\sim}0.827$(평균 0.639), $0.357{\sim}0.780$(평균 0.581)으로 나타났고 FCA240의 marker는 PIC value가 0.70 이상이었다. 또한 PE는 $0.076{\sim}0.444$으로서 10개 marker를 조합시 total PE는 0.9441로 관찰되었다. 10개의 microsatellite DNA다형 좌위를 가지고 친자관계를 분석한 결과 8두 중에서 1두(12.50%)가 모순으로 판정되었다. 또한 국내에서 사육중인 고양이의 개체식별 및 친자판정에 microsatellite marker를 활용할 수 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권6호
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• pp.750-754
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• 2004

This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite markers. The number of alleles per locus varied from 5 to 13 with an average value of 8.56 in the KNH. The observed heterozygosity and the expected heterozygosity ranged 0.398-0.893 (the average value was 0.683) and 0.368-0.871 (the average value was 0.727) in the KNH, respectively. The PIC value and the exclusion probability ranged 0.347-0.853 (the average value was 0.692) and 0.208-0.736, respectively, and the total exclusion probability of 16 microsatellite loci was 0.9999. Of the 16 markers, AHT4, AHT5, ASB2, ASB17, HMS2, HMS3, HTG10, LEX33, TKY321 and VHL20 loci have a relatively high PIC value (>0.7) in the KNH. Of the 19 foals, 5 foals were disqualified by an incompatibility of 4-7 markers according to a Mendelian fashion in the present DNA typing for parentage testing. These results present basic information for developing a system for parentage verification and individual identification in the KNH.