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http://dx.doi.org/10.7235/hort.2013.13026

Construction of a Microsatellite Marker Database of Commercial Pepper Cultivars  

Kwon, Yong-Sham (Variety Testing Division, Korea Seed & Variety Service, Ministry of Agriculture, Food and Rural Affairs)
Hong, Jee-Hwa (Variety Testing Division, Korea Seed & Variety Service, Ministry of Agriculture, Food and Rural Affairs)
Choi, Keun-Jin (Variety Testing Division, Korea Seed & Variety Service, Ministry of Agriculture, Food and Rural Affairs)
Publication Information
Horticultural Science & Technology / v.31, no.5, 2013 , pp. 580-589 More about this Journal
Abstract
This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.
Keywords
Capsicum annuum; genetic relationship; molecular marker; plant variety protection; variety identification;
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