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http://dx.doi.org/10.7235/hort.2014.13194

Use of Microsatellite Markers to Identify Commercial Melon Cultivars and for Hybrid Seed Purity Testing  

Kwon, Yong-Sham (Department of Genetic Engineering, College of Natural Resources and Life Science, Dong-A University)
Hong, Jee-Hwa (Variety Testing Division, Korea Seed and Variety Service, Ministry for Agriculture, Food and Rural Affairs)
Publication Information
Horticultural Science & Technology / v.32, no.4, 2014 , pp. 525-534 More about this Journal
Abstract
Microsatellite markers were used to identify 58 major commercial melon cultivars, and to assess hybrid seed purity of a melon breeding line known as '10H08'. A set of 412 microsatellite primer pairs were utilized for fingerprinting of the melon cultivars. Twenty-nine markers showed hyper-variability and could discriminate all cultivars on the basis of marker genotypes, representing the genetic variation within varietal groups. Cluster analysis based on Jaccard's distance coefficients using the UPGMA algorithm categorized 2 major groups, which were in accordance to morphological traits. The DNA bulks of female and male parents of breeding line '10H08' were tested with 29 primer pairs based on microsatellites to investigate purity testing of $F_1$ hybrid seeds, and 5 primer pairs exhibited polymorphism. One microsatellite primer pair (CMGAN12) produced unambiguous polymorphic bands among the parents. Among 192 seeds tested with CMGAN12, progeny possibly generated by self-pollination of the female parent were clearly distinguished from the hybrid progeny. These markers will be useful for fingerprinting melon cultivars and can help private seed companies to improve melon seed purity.
Keywords
Cucumis melo; genetic purity assessment; genetic relationship; molecular markers;
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1 Anderson, J.A., G.A. Churchill, J.E. Autrigue, and S.D. Tanksley. 1993. Optimizing parental selection for genetic linkage maps. Genome 36:181-186.   DOI   ScienceOn
2 Chiba, N., K. Suwabe, T. Nunome, and M. Hirai. 2003. Development of microsatellite markers in melon (Cucumis melo L.) and their application to major cucurbit crops. Breeding Sci. 53:21-27.   DOI   ScienceOn
3 Danin-Poleg, Y., N. Reis, G. Tzuri, and N. Katzir. 2001. Development and characterization of microsatellite markers in Cucumis. Theor. Appl. Genet. 102:61-72.   DOI   ScienceOn
4 Diaz, A., M. Fergany, G. Formisano, P. Ziarsolo, J. Blanca, Z. Fei, J.E. Staub, J.E. Zalapa, H.E. Cuevas, G. Dace, M. Oliver, N. Boissot, C. Dogimont, M. Pitrat, R. Hofstede, P. van Koert, R. Harel-Beja, G. Tzuri, V. Portnoy, S. Cohen, A. Schaffer, N. Katzir, Y. Xu, H. Zhang, N. Fukino, S. Matsumoto, J. Garcia-Mas, and A.J. Monforte. 2011. A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.). BMC Plant Biol. 11:111.   DOI
5 Fernandez-Silva, I., I. Eduardo, J. Blanca, C. Esteras, B. Pico, F. Nuez, P. Arús, J. Garcia-Mas, and A.J. Monforte. 2008. Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.). Theor. Appl. Genet. 118:139-150.   DOI
6 Gonzalo, M.J., M. Oliver, J. Garcia-Mas, A. Monfort, R. Dolcet-Sanjuan, N. Katzir, P. Arús, and A.J. Monforte. 2005. Simplesequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor. Appl. Genet. 110:802-811.   DOI   ScienceOn
7 International Union for the Protection of New Varieties of Plants (UPOV). 2011. Possible use of molecular markers in the examination of distinctness, uniformity, and stability (DUS). UPOV, Geneva.
8 Kong, Q., C. Xiang, Z. Yu, C. Zhang, F. Liu, C. Peng, and X. Peng. 2007. Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. Mol. Ecol. Notes 7:281-283.   DOI
9 Kwon, Y.S. 2013. Use of EST-SSR markers for genetic characterisation of commercial watermelon cultivars and hybrid seed purity testing. Seed Sci. Technol. 41:245-256.   DOI
10 Kwon, Y.S. and K.J. Choi. 2013. Construction of a DNA profile database for commercial cucumber (Cucumis sativus L.) cultivars using microsatellite markers. Kor. J. Hort. Sci. Technol. 31: 344-351.   과학기술학회마을   DOI   ScienceOn
11 Kwon, Y.S., J.H. Hong, and K.J. Choi. 2013. Construction of a microsatellite marker database of commercial pepper cultivars. Kor. J. Hort. Sci. Technol. 31:580-589.   과학기술학회마을   DOI
12 Pallavi, H.M., R. Gowda, K. Vishwanath, Y.G. Shadakshari, and K. Bhanuprakash. 2011. Identification of SSR markers for hybridity and seed genetic purity testing in sunflower (Helianthus annuus L.). Seed Sci. Technol. 39:259-264.   DOI
13 Li, D., H.E. Cuevas, L. Yang, Y. Li, J. Garcia-Mas, J. Zalapa, J.E. Staub, F. Luan, U. Reddy, X. He, Z. Gong, and Y. Weng. 2011. Syntenic relationships between cucumber (Cucumis sativus L.) and melon (C. melo L.) chromosomes as revealed by comparative genetic mapping. BMC Genomics 12:396.   DOI
14 Liu, G., L. Liu, Y. Gong, Y. Wang, F. Yu, H. Shen, and W. Gui. 2007. Seed genetic purity testing of F1 hybrid cabbage (Brassica oleracea var. capitata) with molecular marker analysis. Seed Sci. Technol. 35:477-486.   DOI
15 Monforte, A.J., J. Garcia-Mas, and P. Arus. 2003. Genetic variability in melon based on microsatellite variation. Plant Breeding 122:153-157.   DOI   ScienceOn
16 Reid, A., L. Hof, G. Felix, B. Rucker, S. Tams, E. Milczynska, D. Esselink, G. Uenk, B. Vosman, and A. Weitz. 2011. Construction of an integrated microsatellite and key morphological characteristic database of potato cultivars on the EU common catalogue. Euphytica 182:239-249.   DOI
17 Ritschel, P.S., T.C. Lins, R.L. Tristan, G.S. Buso, J.A. Buso, and M.E. Ferreira. 2004. Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.). BMC Plant Biol. 4:9.   DOI   ScienceOn
18 Rohlf, F.J. 2000. NTSYSpc: Numerical taxonomy and multivariate analysis system, ver. 2.10b. Applied Biostatistics Inc., New York.
19 Sneath, P.H.A. and R.R. Sokal. 1973. Numerical taxonomy: The principles and practice of numerical classification. Freeman W.H., San Francisco.
20 Wang, F.G., H.L. Tian, J.R. Zhao, H.M. Yi, L. Wang, and W. Song. 2011. Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize cultivars. Maydica 56:7-18.
21 Tomason, Y, P. Nimmakayala, A. Levi, and U.K. Reddy. 2013. Map-based molecular diversity, linkage disequilibrium and association mapping of fruit traits in melon. Mol. Breeding 31:829-841   DOI
22 Zhao, Z., H. Gu, X. Sheng, H. Yu, J. Wang, and J. Cao. 2012. Genetic purity testing of loose-curd cauliflower hybrids using SSR markers and grow out test. Seed Sci. Technol. 40:209-214.   DOI
23 Selvakumar, P., R. Ravikesavan, A. Gopikrishnan, K. Thiyagu1, S. Preetha, and N. Manikanda Boopathi. 2010. Genetic purity analysis of cotton (Gossypium spp.) hybrids using SSR markers. Seed Sci. Technol. 38:358-366.   DOI
24 International Union for the Protection of New Varieties of Plants (UPOV). 2010. Guideline for DNA-profiling: Molecular marker selection and database construction ("BMT guideline"). UPOV, Geneva.
25 Bredemeijer, G.M.M, R.J. Cooke, M.W. Ganal, R. Peeters, P. Isaac, Y. Noordijk, S. Rendell, J. Jackson, M.S. Röder, K. Wendehake, M. Dijcks, M. Amelaine, V. Wickaert, L. Bertrand, and B. Vosman. 2002. Construction and testing of a microsatellite database containing more than 500 tomato cultivars. Theor. Appl. Genet. 105:1019-1026.   DOI   ScienceOn