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http://dx.doi.org/10.5352/JLS.2006.16.3.382

Analysis of Microsatellite Markers for Forensic Identification in cats  

Cho Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Journal of Life Science / v.16, no.3, 2006 , pp. 382-386 More about this Journal
Abstract
A total number of 20 cat samples including 8 parentage testing and 12 individual identification were genotyped. Genomic DNA was extracted from buccal swab, and genotyped by using 10 microsatellite markers (FCA005, FCA26, FCA224, FCA240, FCA453, FCA293, FCA075, FCA105, FCA229, and FCA651). This method consisted of single PCR procedure and showed reasonable amplification of all PCR products. Genotypes were determined by genetic analyzer. The number of alleles per locus of cats varied from 3 to 8 with a mean value of 5.5. Expected heterozygosity was ranged from 0.390 to 0.827 (mean 0.639) and the total exclusion probability of 10 microsatellite loci was 0.9441. Of the 10 markers, FCA240 marker has relatively high PIC value (>0.7). Of the 8 cats, 7 cats were qualified by compatibility according to the Mendelism. These results can give basic information for developing parentage verification and individual identification system in cat.
Keywords
Cat; genotype; identification; microsatellite; parentage verification;
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