• Title/Summary/Keyword: Mass-production

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Production of Cell Mass and Monacolin K from Monascus sp. on Rice Solid Culture (Monascus 속 균주의 균체 생산 및 고체배양에 의한 Monacolin K 생산)

  • 정혁준;유대식
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.160-166
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    • 2004
  • The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$PO$_4$, 0.05% The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % $(KH_2PO_4$, 0.05% $MgSO_4{\cdot}7H_2O$, 0.2% L-asparagine, pH 4.5, and the optimal inoculum size and shaking speed were $1.5{\times}10^6$ spores/50 m1 medium and 150 rpm, respectively. On optimal conditions, 4.1 g/l of the cell mass was obtained at 28$^{\circ}C$ for 3 days. The mycelium were inoculated on 500 g of steamed rice using vinyl bag ($30.6{\times}44$ cm) and incubated at $30^{\circ}C$, 85% humidity for 21 days. Lactone form monacolin K was rapidly increased for 2 days and reached highest concentration of monacolin K (2,930 mg/kg) for 15 days, and monacolin K was decreased after 15 days.

SMOLED equipment for Mass-production

  • Kim, Chang-Woo;Cho, Woo-Seok;Kim, Dong-Soo;Bae, Kyung-Bin
    • 한국정보디스플레이학회:학술대회논문집
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    • 2002.08a
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    • pp.133-136
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    • 2002
  • It is very important to get a stable and large-capacity organic effusion source for achievement of OLED mass-production equipment. We present an organic effusion source with film uniformity less than ${\pm}$ 5%, the material charge volume, 300cc for $400{\times}400\;mm^2$ substrate. The fine metal shadow mask alignment technology, one of the color forming technique, also have to support more accurate and fast operating in mass-production. In this paper, we will describe the OLED mass-production equipment with the large volume effusion source and the precision shadow mask alignment technique.

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Optimization of Culture Medium for Lactosucrose ($^4G-{\beta}$-D-Galactosylsucrose) Production by Sterigmatomyces elviae Mutant Using Statistical Analysis

  • Lee, Jong-Ho;Lim, Jung-Soo;Song, Yoon-Seok;Kang, Seong-Woo;Prak, Chul-Hwan;Kim, Seung-Wook
    • Journal of Microbiology and Biotechnology
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    • v.17 no.12
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    • pp.1996-2004
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    • 2007
  • In this study, the optimization of culture medium using a Sterigmatomyces elviae mutant was investigated using statistical analysis to increase the cell mass and lactosucrose ($^4G-{\beta}$-D-galactosylsucrose) production. In basal medium, the cell mass and lactosucrose production were 4.12 g/l and 140.91 g/l, respectively. However, because of the low cell mass and lactosucrose production, optimization of culture medium was carried out to increase the cell mass and lactosucrose production. Culture media were optimized by the S. elviae mutant using analysis of variance (ANOVA) and response surface methodology (RSM). Central composite designs using RSM were utilized in this investigation. Quadratic models were obtained for cell mass and lactosucrose production. In the case of cell mass, optimal components of the medium were as follows: sucrose 1.13%, yeast extract 0.99%, bactopeptone 2.96%, and ammonium sulfate 0.40%. The predicted maximum value of cell mass was about 5.20 g/l and its experimental value was 5.08 g/l. In the case of lactosucrose production, optimal components of the medium were as follows: sucrose 0.96%, yeast extract 1.2%, bactopeptone 3.0%, and ammonium sulfate 0.48%. Then, the predicted maximum value of lactosucrose production was about 194.12 g/l and the corresponding experimental value was about 183.78 g/l. Therefore, by culturing using predicted conditions, the real cell mass and lactosucrose production increased to 23.3% and 30.42%, respectively.

A study on the mass production performance evaluation of servomotors for manufacturing robots (제조 로봇용 서보모터의 양산성능 평가 연구)

  • Ryu, K.H.;Lee, T.H.;Park, J.W.;Kwon, H.S.;Kang, B.S.
    • Journal of Drive and Control
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    • v.19 no.4
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    • pp.91-96
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    • 2022
  • The performance and reliability of foreign servomotors and domestic servomotors for manufacturing robots were compared and verified. The mass productivity was confirmed by satisfying the requirements of the demanding companies. The servomotor was designed in consideration of mass productivity while satisfying the requirements of the demanding company. It was mounted on a real robot to check its performance and reliability. To secure mass-production performance, we designed and manufactured easy-to-manufacture parts and jigs to manufacture servomotors and achieved the goal of the mass-production evaluation of servomotors through individual performance evaluation, robot mounting test evaluation, and supplementation.

A Study on The Mass Production Weapon System Parts Localization System Engineering Development Management Process Application based on ISO/IEC/IEEE 15288 (ISO/IEC/IEEE 15288 기반 양산단계 무기체계 부품국산화 체계공학 개발관리 절차 적용 연구)

  • Kim, Jang-Eun;Shim, Bo-Hyun;Cho, Yu-Seup;Sung, In-Chul;Han, Dong-Seog
    • Journal of Korean Society for Quality Management
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    • v.44 no.3
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    • pp.541-552
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    • 2016
  • Purpose: In this study, we propose that how to approach a effective system engineering and optimize system engineering management process for the mass production weapon system parts localization development process and success in DTaQ. Methods: To approach a effective system engineering for the mass production weapon system parts localization, we analyze a weapon system acquisition process and system engineering process of Republic of Korea and DTaQ parts localization business regulations in advance. after results of analysis of them, we implement a optimized parts localization development system engineering based on ISO/IEC/IEEE 15288. Results: In order to apply International Standard ISO/IEC/IEEE 15288 to the mass production weapon system parts localization development process, we compare the mass production weapon system parts localization acquisition environment with ISO/IEC/IEEE 15288 and analyze them. therefore, It is possible to implement a part of concept stage and development stage of ISO/IEC/IEEE total life cycle stage for the mass production weapon system parts localization development process. To achieve the technical review milestones of DTaQ parts localization business regulations in the selected stages of ISO/IEC/IEEE, the development and management agency perform 2 high rank process and 19 low rank process specified in ISO/IEC/IEEE. Conclusion: When the development and management agency perform the mass production weapon system parts localization development using the proposed system engineering approach, they should easily meet milestone through the clarified requirement and simplified System Engineering output documents in limited development period.

A Study on the Role of Statistics in Industrial mass production -Standardization production·Inspection- (공산품생산(工産品生産)에 있어 통계학(統計學)의 역할(役割)에 관한 연구(硏究) -표준화(標準化)·생산(生産) 검사(檢査)-)

  • Kim, jong-ho
    • Journal of Korean Society for Quality Management
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    • v.5 no.2
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    • pp.3-20
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    • 1977
  • The purpose of this Study is to develope the Role of Statistics in Industrial mass production. The process of mass production will be divided into three steps, that is, Standardization, production and inspection. The Statistics is applied to Specificat-ions, Quality Control and Sampling inspection in these three steps. The applications have developed to Statistical methods based on probability theory. And then, The improved plan is exhibited the point of problems of introducting of spreading of quality control throughout field survey.

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Effects of Cadmium on Radial Growth and Dry Mass Production of Ectomycorrhizal Fungi

  • Kim, Chang-Gi;Power, Sally Anne;Bell, John Nigel Berridge
    • The Korean Journal of Ecology
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    • v.27 no.5
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    • pp.301-306
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    • 2004
  • The sensitivity to Cd of three ectomycorrhizal fungi, Paxillus involutus, Suillus bovinus and Rhizopogon subcaerulescens, was assessed and compared in terms of radial growth and dry mass production, using both agar and liquid culture. The radial growth of S. bovinus and R. subcaerulescens was significantly reduced at the lowest concentration (0.1mg Cd/L). The 50% effective concentration (EC$_{50}$) values calculated from radial growth rates of the ectomycorrhizal fungi showed that the sensitivity of the fungi to Cd was greatest in S. bovinus and lowest in R. subcaerulescens. Cadmium addition also significantly decreased dry mass production of the ectomycorrhizal fungi. The sensitivity of the fungi to Cd in terms of dry mass production, was greatest in S. bovinus and lowest in P. involutus. Higher growth rates of P. involutus and melanisation of R. subcaerulescens appeared to contribute to reduced Cd toxicity.

Study of 'Education-Training-Certificate of Qualification' Design for the Fashion Accessories Production Based on the National Competency Standards (패션소품생산 분야의 국가직무능력표준 기반 교육훈련자격 설계에 관한 연구)

  • Suh, Seunghee;Lee, Shin-Young
    • Journal of the Korean Society of Costume
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    • v.65 no.2
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    • pp.125-143
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    • 2015
  • This study is to propose an 'education-training-certificate of qualification' design of fashion accessories production, which can be applied to education in universities and individuals. It is based on the National Competency Standards (NCS), which was developed through the '2013 National Competency Standards Development Project' for the fashion accessories production. FGI (Focus Group Interviews), which is a research methodology, is carried out on target groups of educational experts and specialists in the field of fashion accessories production. Through this, five courses were suggested; first, 'fashion accessories design' course was proposed for the education and training of 'design development' and 'development of raw materials'. Secondly, 'fashion product production' course was proposed for the education and training of 'production of samples'. Thirdly, 'fashion product manufacture and planning' course was proposed for the competency element units: 'calculation of cost', 'determination of mass production model and price', 'planning of the main manufacturing process' and 'ordering of raw materials'. Lastly, 'mass production of fashion products' course and the 'field practice of fashion product manufacture' course were proposed for the competency element units: 'planning for mass production', 'preparation for mass production', 'mass production' and 'inspection of completed products'. In addition, a new certificate of 'technician of fashion accessory production' was proposed in order to test qualified skills for the fashion accessories production. The test is composed of a written examination of short-answer questions, technical drawing and production.

Activity-oriented Modeling of Mass Production System (대량생산 체제의 Simulation을 위한 Activity 중심 Modeling)

  • Choe, Byeong-Gyu;Park, Seong-Ju;Sin, Ha-Yong
    • Journal of Korean Institute of Industrial Engineers
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    • v.12 no.1
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    • pp.119-131
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    • 1986
  • Described in this paper is a modelling methodology for mass production system simulation. The mass production system under consideration consists of various types of flow lines, special purpose production facilities, conveyor lines, palletized carts, and storage facilities. This type of production system is typical in home appliance industry, automobile industry, footwear industry, etc. where a variety of product mix are mass-produced. The modelling methodology is based on the "discrete-event formalism", and an "activity-oriented world view" is adopted to formalize the system description. A distinctive feature of the modelling methodology is that only the static structure (ie, system components) is included in the fixed model. The dynamic structure of the system is specified through a "data-driven" mechanism, which is an extension of the "experimental frame" concept. Each type of system components (ie, flow line, conveyors, carts, etc.) is formally modeled by using Activity Cycle Diagrams. The issue of "model structuring" is also addressed. The modeling methodology has been successfully applied in a real simulation study of a mass production system.

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Effects of Media on the Callogenensis and Cell Mass Production in Cell Cultures of Panax vietnamensis

  • Luong, Hoang Van;Long, Nguyen Van;Duong, Vu Binh;Toan, Nguyen Linh;Minh, Nguyen Van;Quang, Le Bach;Kim, Nam-Hyuck;Byun, Sang-Yo
    • KSBB Journal
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    • v.24 no.2
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    • pp.177-181
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    • 2009
  • This study was initiated to investigate the impacts of media types and other components on the callogenensis and cell mass production of Panax vietnamensis in the first step of the cell biomass procedure. Four media were checked: Murashige-Skoog (MS), White, Gamborg and Nitch-AII. All the four media were shown potential media for Panax vietnamensis callogenensis and cell mass production, in which the MS medium showed the best results: the successful callogenensis ratio and cell mass formation were 30% and 62,93 ${\pm}$ 3,63 mg (DW) respectively, the Nitch medium showed the lowest results: the successful callogenensis ratio and cell mass formation were 15% and 27,10 ${\pm}$ 2,24 mg (DW) respectively. The results showed that the MS medium is the most suitable medium for Panax vietnamensis callogenensis and cell mass production.