• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.12
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• pp.141-146
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• 2016

HEVC/H.265 is the latest joint video coding standard proposed by ITU-T SG 16 WP and ISO/IEC JTC 1/SC29/WG 11. In H.265, pictures are divided into a sequence of coding tree units(CTUs), and the CTU further is partitioned into multiple CUs to adapt to various local characteristics. Its coding efficiency is approximately two times high compared to previous standard H.264/AVC. However according to the size of extended CU(coding unit) and transform block, the hardware size of PMR(prediction/mode decision/reconstruction) block within video encoder is about 4 times larger than previous standard. In this study, we propose a new less complex hardware architecture of PMR block which has the most high complexity within encoder without any noticeable PSNR loss. Using this simplified block, we can shrink the overall size the H.265 encoder. For FHD image, it operates at clocking frequency of 300 MHz and frame rate of 60 fps. And also for the test image, the Bjøntegaard Delta (BD) bit rate increase about average 30 % in PMR prediction block, and the total estimated gate count of PMR block is around 1.8 M.

• Journal of IKEEE
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• v.2 no.1 s.2
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• pp.1-14
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• 1998

In this paper, Antenna, LNA, Mixer, VCO, and Modulation/Demodulation in Baseband processor which are the RF main components in Wireless LAN system for ultra high-speed communications network are studied. Antenna bandwidth and selective fading due to multipath can be major obstacles in high speed digital communications. To solve this problem, wide band MSA which has loop-structure magnetic antenna characteristics is designed. Distributed mixer using dual-gate GaAs MESFET can achieve over 10dB LO/RF isolation without hybrid, and minimize circuit size. As linear mixing signal is produced, distortions can be decreased at baseband signals. Conversion gain is achieved by mixing and amplification simultaneously. Mixer is designed to have wide band characteristics using distributed amplifier. In VCO design, Oscillator design method by large signal analysis is used to produce stable signal. Modulation/Demodulation system in baseband processor, DS/SS technique which is robust against noise and interference is used to eliminate the effect of multipath propagation. DQPSK modulation technique with M-sequences for wideband PN spreading signals is adopted because of BER characteristic and high speed digital signal transmission.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.2
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• pp.219-226
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• 2010

The stamping process is widely used in fabricating various sheet-parts for vehicle, airplane, and electronic devices due to its low processing cost and high productivity. Recently the use of thin sheets with corrugated structures has rapidly increased for the production of energy devices, e.g., heat exchangers and fuel cells. However, it is very difficult to make corrugated structures directly in the stamping process due to their geometrical complexity. To solve this problem, this paper proposes a multi-step stamping process with a combined heat treatment process: a sequence of the first stamping, heat treatment, and second stamping. By multi-stamping, we obtained successful results in fabricating very thin corrugated structures with thicknesses of $100{\mu}m$; these are applicable as part of a plate-type heat exchanger.

• The Journal of the Korea Contents Association
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• v.10 no.2
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• pp.72-80
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• 2010

The 1Gbps clock and data recovery (CDR) circuit using the proposed multi-point phase detector (PD) is presented. The proposed phase detector generates up/down signals comparing 3-point that is data transition point and clock rising/falling edge. The conventional PD uses the pulse width modulation (PWM) that controls the voltage controlled oscillator (VCO) using the width of a pulse period's multiple. However, the proposed PD uses the pulse number modulation (PNM) that regulates the VCO with the number of half clock cycle pulse. Therefore the proposed PD can controls VCO preciously and reduces the jitter. The CDR circuit is tested using 1Gbps $2^{31}-1$ pseudo random bit sequence (PRBS) input data. The designed CDR circuit shows that is capable of recovering clock and data at rates of 1Gbps. The recovered clock jitter is 7.36ps at 1GHz and the total power consumption is about 12mW. The proposed circuit is implemented using a 0.18um CMOS process under 1.8V supply.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.1
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• pp.57-65
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• 1997

Cytogenetic observations of loss of the distal portion of the Y chromosome long arm were found to be associated with disrupted spermatogenesis. The existence of a gene involved in the regulation of spermatogenesis, the azoospermia factor (AZF), was postulated. In this study, we screened the AZF region including DAZ and DAZH genes and observed the expression pattern of DAZ and DAZH transcript in infertile men with azoospermia and oligospermia by using a sequence-tagged site (STS)-based PCR method. PCR primers were synthesized for 11 STSs that span Yq interval 6, SRY, DAZ, and DAZH, functional DAZ homologue on chromosome 3. Microdeletions were detected in 4/32 (12.5%) azoospermic men and 1/11 (9%) severe oligospermic men. Only 2 of 5 patients had microdeletions of Yq that contained the DAZ gene, whereas the other 3 patients had deletions extending from intervals 5L-6F proximal to the DAZ gene on Yq. Testis biopsies of the azoospermic patients revealed a variety from Sertoli cell-only syndrome to testicular maturation arrest. Of 4 men with clinical data available, average testis size was R: 13.8 cc, L: 13.8 cc, serum T was $4.0{\pm}1.25$ ng/ml, LH was $3.63{\pm}1.90$ mIU/ml, and FSH was $8.85{\pm}5.13$ mIU/ml. These values did not differ significantly from the remainder of the patients tested. We could not observed the DAZ transcript in 2 patients, who have no mature spermatozoa. In 11.6% of patients microdeletions of the AZF could be detected. These deletions in the AZF region seem to be involved causing spermatogenic failure. But the frequency of microdeletions proximal to DAZ suggests that DAZ is not the only gene associated with spermatogenic failure.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.9
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• pp.1334-1341
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• 2007

This study identified hepatic differentially expressed genes (DEGs) affecting the marbling of muscle. Most dietary nutrients bypass the liver and produce plasma lipoproteins. These plasma lipoproteins transport free fatty acids to the target tissue, adipose tissue and muscle. We examined hepatic genes differentially expressed in a differential-display reverse transcription-polymerase chain reaction (ddRT-PCR) analysis comparing high- and low-marbled Hanwoo steers. Using 60 arbitrary primers, we found 13 candidate genes that were upregulated and five candidate genes that were downregulated in the livers of high-marbled Hanwoo steers compared to low-marbled individuals. A BLAST search for the 18 DEGs revealed that 14 were well characterized, while four were not annotated. We examined four DEGs: ATP synthase F0, complement component CD, insulin-like growth factor binding protein-3 (IGFBP3) and phosphatidylethanolamine binding protein (PEBP). Of these, only two genes (complement component CD and IGFBP3) were differentially expressed at p<0.05 between the livers of high- and low-marbled individuals. The mean mRNA levels of the PEBP and ATP synthase F0 genes did not differ significantly between the livers of high- and low-marbled individuals. Moreover, these DEGs showed very high inter-individual variation in expression. These informative DEGs were assigned to the bovine chromosome in a BLAST search of MS marker subsets and the bovine genome sequence. Genes related to energy metabolism (ATP synthase F0, ketohexokinase, electron-transfer flavoprotein-ubiquinone oxidoreductase and NADH hydrogenase) were assigned to BTA 1, 11, 17, and 22, respectively. Syntaxin, IGFBP3, decorin, the bax inhibitor gene and the PEBP gene were assigned to BTA 3, 4, 5, 5, and 17, respectively. In this study, the in silico physical maps provided information on the specific location of candidate genes associated with economic traits in cattle.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.3667-3671
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• 2015

Invasion and metastasis is the major cause of tumor recurrence, difficulty for cure and low survival rate. Excavating key transcription factors, which can regulate tumor invasion and metastasis, are crucial to the development of therapeutic strategies for cancers. PU.1 is a master hematopoietic transcription factor and a vital regulator in life. Here, we report that, compared to adjacent non-cancerous tissues, expression of PU.1 mRNA in metastatic hepatocellular carcinoma (HCC), but not primary HCC, was significantly down-regulated. In addition, levels of PU.1 mRNA in metastatic hepatoma cell lines MHCC97L and MHCC97H were much lower than in non-metastatic Hep3B cells. Transwell invasion assays after PU.1 siRNA transfection showed that the invasion of hepatoma cell lines was increased markedly by PU.1 knockdown. Oppositely, overexpression of PU.1 suppressed the invasion of these cells. However, knockdown and overexpression of PU.1 did not influence proliferation. Finally, we tried to explore the potential mechanism of PU.1 suppressing hepatoma cell invasion. ChIP-qPCR analysis showed that PU.1 exhibited a high binding capacity with miR-615-5p promoter sequence. Overexpression of PU.1 caused a dramatic increase of pri-, pre- and mature miR-615-5p, as well as a marked decrease of miR-615-5p target gene IGF2. These data indicate that PU.1 inhibits invasion of human HCC through promoting miR-615-5p and suppressing IGF2. These findings improve our understanding of PU.1 regulatory roles and provided a potential target for metastatic HCC diagnosis and therapy.

• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.80-89
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• 1996

The expression and secretion of human lactoferrin (hLf) in Sacclnromyces diastaticus were performed. 1. For the secretion of hLf in yeast, recombinant plasmid pYEGLf was constructed using promoter, secretion signal sequence of glucoamylase I gene (STA1) and transcriptional terminator of GAL7 gene. 2. Each correct recombinant plasmid was selected by mini-preparation of plasmid DNA from E coli transformant and restriction enzyme digestion analysis. The selected plasmids, pYEGLf, were transformed into S. diastaticus YIY345 as a expression host, respectively. 3. Western blot analysis using rabbit anti-hLf was carried out to identify expressed hLf. Positive signals were shown in culture supernatant of pYEGLf transformant. 4. About $100{\mu}g-1mg$ of concentrated culture supernatant of positive clone were loaded on paper disc and tested for the antimicrobial activity against E coli. However, no activity was observed. We concluded that this fact results from low concentration of hLf secreted from yeast, compared with the fact that MIC of hLf is as high as $3mg/m{\ell}$. Therefore, the purification of secreted hLf may be require to investigate the antimicrobial activity. From this study, the feasibility of low-cost production of sufficient quantities of human lactofferin for nutritional and therapeutical applications were suggested.

• Economic and Environmental Geology
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• v.9 no.1
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• pp.1-11
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• 1976

It is believed that geological survey, drilling and geophysical survey which was carried out on Tertiary deposits in Pohang is a valuable but through the studying of many Tertiary sediments in Japan discovered many questions on analysis of final report prepared by National geological survey. The main reason is: 1. The seismic sound velocity which have regulated in the final report prepared by geological survey for Tertiary deposits in Pohang was 1,500-2,000m/sec in spite of oil bearing sediments of same age in Japan are 2,000-3,800m/sec. These may means the requirement of reconsideration of seismic velocity for Tertiary deposits in Pohang and required to have a dipper drilling. 2. Stratigraphically, geophysically, and paleontologically, the Tertiary deposits in Pahang land area is similar with that of Nishiyama-Hunakawa formations of Akita oil field in Japan. Nishiyama-Hunakawa formation is the main oil bearing formation in Japan. 3. Those valcanic rock including andestitic rock and liparitic rock which have extensively distributed over either at land area or at sea bottom, assumed by geological survey as the base of Tertiary sediments. But in case of Japan many oil bearing deposits are in over laid by these kind of volcanic rock. Therefore a possible of same condition with Japan is presumable on Tertiary sediments in Pohang. 4. It is believed that the Tertiary sediments of land area in Pohang is the extension of offshore basin but is wandering that the final report submitted by geologic survey have not remain any word on report of ECAFE discribed so much problematics as followed: A. Although it was assumed that no great thickness exceeding 1,000 meters, or major structures would be encountered in the Tertiary offshore sequence, it was hoped that shallow hydrocarbon deposits might be found, because these sediment are lithologically similar to those of the same age in the producing area of the northwest Honshu region of Japan where hydrocarbon are extracted from depths of only 500 to 600 meters. B. Four possible hydrocarbon trap conditions are represented in the survey area: anticlinal folds, faults, pinch outs, along the igneous basement and lateral facies changes. C. Most of the prime possible reservoir area are beyond the 50 meter water depth mark, except for the structures in Yonil Bay. D. Despite the shallowness of the offshore basin, sufficient trap condition exist in the area to warrant further exploration for hydrocarbon. 5. All of the problems mentioned above have gave us a strong reasons to have us hesitating to make a final conclusion on Tertiary problems in Pohang, before to have a drill to a depth to 3,000 meters or more whatever it is the Tertiary or a Mesozoics below 1,000 meters.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.286-293
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• 2012

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles ($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, $L^0L^0$), C. annuum Tisana ($L^1L^1$), C. annuum Criollo de Morelos 334 (CM334, $L^2L^2$), Capsicum chinense PI 159236 ($L^3L^3$), and Capsicum chacoense PI 260429 ($L^4L^4$). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the $L^3$-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 $F_1$ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes ($P_0$, $P_1$, and $P_{1,2$). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.