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http://dx.doi.org/10.7235/hort.2012.12018

A Set of Allele-specific Markers Linked to L Locus Resistant to Tobamovirus in Capsicum spp.  

Lee, Jun-Dae (Research and Development Unit, Pepper and Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Han, Jung-Heon (Research and Development Unit, Pepper and Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Yoon, Jae-Bok (Research and Development Unit, Pepper and Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Horticultural Science & Technology / v.30, no.3, 2012 , pp. 286-293 More about this Journal
Abstract
The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles ($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, $L^0L^0$), C. annuum Tisana ($L^1L^1$), C. annuum Criollo de Morelos 334 (CM334, $L^2L^2$), Capsicum chinense PI 159236 ($L^3L^3$), and Capsicum chacoense PI 260429 ($L^4L^4$). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the $L^3$-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 $F_1$ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes ($P_0$, $P_1$, and $P_{1,2$). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.
Keywords
CAPS; $L^3$; $L^4$; PMMoV; SCAR;
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