• 한국환경농학회지
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• 제33권3호
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• pp.189-193
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• 2014

BACKGROUND: The aim of this study is to investigate the effects of Lycium chinense Miller fruit and its representative constituent betaine on reduction of immobility time and blood parameters in balb/c mice. METHODS AND RESULTS: We investigated the immobility time and the changes in aspects of blood biochemical parameters by the administration of L. chinense Miller fruit and its representative constituent betaine, after the forced swimming test. The immobility time was significantly reduced about 41.3% and 53.6%, respectively, in the animal of L. chinense Miller fruit and its representative constituent betaine-administrated group for 7 days, in comparison with that of the control group. The level of blood urea nitrogen was significantly decreased in L. chinense Miller fruit and its representative constituent betaine-treated group compared with the control group (P < 0.05), respectively. In addition, the interlukin-2 levels of mice in L. chinense Miller fruit and betaine treated group was increased compared with the control group. CONCLUSION: These results indicate that L. chinense Miller fruit and betaine might be helpful in the immune function improvement, enhance physical stamina, and fatigue recovery.

• Preventive Nutrition and Food Science
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• 제9권1호
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• pp.95-97
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• 2004

An angiotensin converting enzyme (ACE) inhibitory substance was isolated and purified from Lycium chinense Miller. A crude water extract of Lycium chinense Miller was prepared by adding it to water shaking at $25^{\circ}C$ for 1 hr, followed by centrifugation at 8000 ${\times}$ g for 30 min. The crude extract was then filtered using YM-3 and YM-1 membranes. An ACE inhibitor was isolated using consecutive chromatographic methods including: ion exchange chromatography, gel permeation chromatography, and FPLC. The inhibitor was identified to have a molecular mass of 862 daltons by mass spectrometry.

• Archives of Pharmacal Research
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• 제8권4호
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• pp.249-252
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• 1985

$N_{9}$-Formylharman, 1-carbomethoxy-$\beta$-carboline and perlolyrine with an unidentified alkaloid ($C_{10}H_{13}NO_{2}$, $M^{+}$ 179.094) have been isolated from the fruits of Lycium chinense Miller (Solanaceae) and characterized on the basis of chemical and physical evidence.

• 한국응용과학기술학회지
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• 제39권1호
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• pp.27-33
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• 2022

본 연구는 구기자의 화장품 소재로서의 가능성을 검토하였다. 에탄올을 용매로 하여 얻어진 추출물 중 총 폴리페놀 함량은 17.3 mg/mL이었으며, 추출물의 세포 독성을 확인하기 위해 MTT assay결과 1000 ㎍/mL 이하의 농도에서 세포 생장에 영향을 미치지 않았다. 항산화 효능 실험으로 DPPH radical 소거 시험을 실시한 결과 1000 ㎍/mL의 농도에서 84.97%의 소거능을 나타내었고, SOD 유사 활성 실험 결과 1000 ㎍/mL에서 80.54%의 결과로 우수한 항산화 효능을 보였다. Elastase 저해 활성을 측정한 결과 1000 ㎍/mL에서 50.93%의 저해율을 나타내어 구기자 추출물이 elastase 저해에 효과가 있음을 확인할 수 있었다. 본 연구로부터 구기자 70% ethanol 추출물은 항산화와 주름 개선 효능을 확인함으로써 기능성 화장품 소재로의 활용 가능성을 확인하였다.

• Journal of Biosystems Engineering
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• 제35권4호
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• pp.244-249
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• 2010

This study was conducted to define the optimal Lycium chinense miller washing method for developing the Lycium chinense cleaner and we analyzed the Lycium chinense miller washing characteristics for removing pesticides and microorganism according to washing methods; habitual washing method, air bubble washing method and nozzle spray washing method. The results were summarized as follows; 1. In case of measuring physical properties according to the varieties, maximum yield strength of Hokwang was 2.562 kgf, minimum yield strength of Hokwang was 0.269 kgf and average yield strength was about 1 kgf. 2. In case of measuring change of bacteria according to washing methods, the number of bacteria of non-washing method was more than the number of bacteria of habitual washing method or mechanical washing method and the number of nozzle spray washing method was least. 3. Ahjoksiseuteurobin of 0.218 ppm was detected in the untreated sample, 0.051 ppm was detected in the habitual washing method, 0.047 ppm was detected in the air bubble washing method and 0.034 ppm was detected in nozzle spray washing method. Every amount detected were less than 2 ppm that is reference value and the detected amount was least in case of nozzle spay cleaning method. Cypermethrin of 0.772 ppm was detected in the non-cleaned sample, 0.089ppm was detected in habitual washing method, 0.26 ppm was detected in the air bubble washing method and 0.292 ppm was detected in the nozzle spray washing method. Every detected amount of Cypermethrin were less than 5 ppm that is reference value and the detected amount was least in case of habitual washing method.

• Archives of Pharmacal Research
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• 제28권9호
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• pp.1031-1036
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• 2005

[ $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-glucopyranoside$ ] (1) was isolated from an ethyl acetate extract of the root bark from Lycium chinense Miller, and its structure was determined using 1D and 2D NMR spectroscopy including DEPT, HMQC, and HMBC. $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-glucopyranoside$ exhibited potent antimicrobial activity against antibiotic-resistant bacterial strains, methicillin-resistant Staphylococcus aureus (MRSA) isolated from patients, and human pathogenic fungi without having any hemolytic effect on human erythrocytes. In particular, compound 1 induced the accumulation of intracellular trehalose on C. albicans as stress response to the drug, and disrupted the dimorphic transition that forms pseudo-hyphae caused by the pathogenesis. This indicates that $(+)-Lyoniresinol-3{\alpha}-O-\beta-D-glucopyranoside$ has excellent potential as a lead compound for the development of antibiotic agents.

• Archives of Pharmacal Research
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• 제25권4호
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• pp.433-437
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• 2002

A new phenolic amide, dihydro-N-caffeoyltyramine (1) was isolated from the root bark of Lycium chinense Miller, along with known compounds, trans-N-caffeoyltyramine (2), cis-N-caffeoyltyramine (3), and lyoniresinol $3{\alpha}-Ο-{\beta}-D-glucopyranoside$ (4). Their structures were determined by spectroscopic analysis. A NBT superoxide scavenging assay revealed that three phenolic amides showed potent antioxidative activity.

• Journal of Plant Biotechnology
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• 제31권4호
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• pp.295-300
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• 2004

구기자나무의 잎 절편을 A. rhizogenes strain, 15834와 공동 배양하여 유도된 모상근의 최적배양조건을 결정하기 위하여 MS배지와 sucrose의 농도를 다양하게 처리한 결과, 1/2 MS 배지에서 생체중 (12.3 g)과 건물중 (1.4 g)으로 가장 높게 나타났으며, 탄소원으로 사용된 sucrose에서는 3% 농도에서 생체중과 건물중이 가장 양호하게 나타났다. 이와 같이 결정된 MS농도와 sucrose의 농도를 이용하여 대량배양을 하고자 10 L의 air lift bioreactor에서 대량 배양한 결과 역시 1/2 MS와 3% sucrose의 농도를 사용한 처리구에서 가장 높은 생체중과 건물중의 증가를 보였다. 하지만, 1/2 MS에 5% sucrose를 처리한 bioreactor배양에서도 3% sucrose처리에서와 마찬가지로 생체중과 건물중이 높게 나타났다.

• 한국유기농업학회지
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• 제20권1호
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• pp.91-99
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• 2012

This study was conducted to develop a functional feed additive for pig with spent $Lycium$ $chinense$ Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent $Lycium$ $chinense$ Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression ($p$ = 0.001), linear ($p$ = 0.002), square ($p$ = 0.017) and interaction ($p$ = 0.047) was significant and adjusted determination coefficient ($R^2$) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.

• 한국자원식물학회지
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• 제1권1호
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• pp.72-79
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• 1988

This experiment was conducted to breed the Boxthorn varieties with resistance to Anthracnose, good quality and high yielding potential in 1984 to 1986(3 years).The character of "Yu seong 1" with resistance to Anthracnose and "Yu seong 2"with good quality and high yielding potential are fellows;1, The plant type of "Yu seong 1" was semi-elect type but became to electtype after 60C" rediation and yield was decreased because of the number of flowers.2. The yield of "Yu seong 2" was the highest of all varieties.3. The quality(Extract and Betain contents) of "Yu seong 2" was the best ofall varieties and visible character was good.The results of this experinent showed that "Yu seong 1" should be promisinglines in south area because of resistance Anthracnose and "Yu seong 2" should bepromising lines in north area.hracnose and "Yu seong 2" should bepromising lines in north area.orth area.