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http://dx.doi.org/10.5010/JPB.2004.31.4.295

Induction of Hariy Root and Bioreactor Culture of Lycium chinense  

Bae, Ki-Hwa (마이크로프랜츠 부설연구소)
Kim, Yun-Soo (중앙대학교 인삼산업연구센터)
Jeong, Jae-Hun (국립산림과학원 생물공학과)
Kim, Young-Seon (남도대학 약용자원원예개발과)
Choi, Yong-Eui (강원대학교 산림과학대학 산림자원부)
Yoon, Eui-Soo (공주대학교 생물학과)
Publication Information
Journal of Plant Biotechnology / v.31, no.4, 2004 , pp. 295-300 More about this Journal
Abstract
This article was conducted to induce the transgenic hairy roots and determine the effect of culture conditions on optimum growth of hairy roots by Agrobacterium rhizogenes strain, 15834 in Lycium chinense Miller. Hairy roots of L. chinense Miller. were induced from leaf segments by co-cultivation with A. rhizogenes. When the hairy roots were cultured in various MS medium strength and sucrose concentrations, the highest growth of hairy roots was observed in half-strength MS media supplemented with 3% sucrose, respectively. In air lift bioreactor cultures, the liquid medium contained with 1/2 MS and 3% sucrose was also the best for optimum growth of hairy roots.
Keywords
Bioreactor; co-cultivation; Lycium chinense Miller; MS medium strength; roIC gene; sucrose;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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