Browse > Article

Optimization of Aqueous Methanol Extraction Condition of Total Polyphenol from Spent $Lycium$ $chinense$ Miller to Develop Feed Additives for Pig  

Shim, Kwan-Seob (Department of Animal Biotechnology, Chonbuk National University)
Na, Chong-Sam (Department of Animal Biotechnology, Chonbuk National University)
Oh, Sung-Jin (Department of Animal Science, Chonbuk National University)
Choi, Nag-Jin (Department of Animal Science, Chonbuk National University)
Publication Information
Korean Journal of Organic Agriculture / v.20, no.1, 2012 , pp. 91-99 More about this Journal
Abstract
This study was conducted to develop a functional feed additive for pig with spent $Lycium$ $chinense$ Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent $Lycium$ $chinense$ Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression ($p$ = 0.001), linear ($p$ = 0.002), square ($p$ = 0.017) and interaction ($p$ = 0.047) was significant and adjusted determination coefficient ($R^2$) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.
Keywords
spent Lycium chinense Miller; aqueous methanol; total polyphenol; response surface methodology; fractional factorial design;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Lonni, A. A. S. G., R. Longhini, G. C. Lopes, J. C. P. de Mello, and I. S. Scarminio. 2012. Statistical mixture design selective extraction of compounds with antioxidant activity and total polyphenol content from Trichilia catigua. Anal. Chim. Acta 719: 57-60.   DOI
2 Mussatto, S. I., L. F. Ballesteros, S. Martins, and J. A. Teixeira. 2011. Extraction of antioxidant phenolic compounds from spent coffee grounds. Sep. Purif. Technol. 83: 173-179.   DOI
3 Oliveira, R. A., C. D. Narciso, R. S. Bisinotto, M. C. Perdomo, M. A. Ballou, M. Dreher, and J. E. P. Santos. 2010. Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves. J. Dairy Sci. 93: 4280-4291.   DOI   ScienceOn
4 Rice-Evans, C. A., N. Miller, and G. Paganga. 1997. Antioxidant properties of phenolic compound. Trends in Plant Science 2: 152-159.   DOI   ScienceOn
5 Soni, P., M. Singh, A. L. Kamble, and U. C. Banerjee. 2007. Response surface optimization of the critical medium components for carbonyl reductase production by Candida viswanathii MTCC 5158. Bioresour. Technol. 98: 829-833.   DOI   ScienceOn
6 Zhang, R., K. A. Kang, M. Piao, K. Kim, A. Kim, S. Chae, J. Park, U. Youn, and J. Hyun. 2010. Cytoprotective effect of the fruits of Lycium chinense Miller against oxidative stress-induced hepatotoxicity. J. Ethnopharmacol. 130: 99-306.
7 Box, G. E. P. and D. W. Behnken. 1960. Some new three level designs for the study of quantitative variables. Technometrics 2: 455-475.   DOI   ScienceOn
8 Chang, W.-K., S.-B. Cho, D.-W. Kim, S.-S. Lee, and S.-K. Kim. 2010. Cell Growth and Antioxidant Activity on Onion Juice Fermentation by Using Lactobacillus plantarum as Animal Probiotics. J. Life Sci. 20: 1729-1737.   DOI
9 Chirinos, R., H. Rogez, D. Campos, R. Pedreschi, and Y. Larondelle. 2007. Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruiz & Pavon) tubers. Sep. Purif. Technol. 55: 217-225.   DOI   ScienceOn
10 Cho, S. B., W. K. Chang, Y. J. Kim, H. I. Moon, J. W. Joo, K. H. Seo, and S. K. Kim. 2010. Effect of plant oils and minerals for the inhibition of lipase activity of Staphylococcus aureus isolated from fermented pork meat. Korean J. Food Sci. Ani. Resour. 30: 764-772.   DOI
11 Cohran, W. and G. Cox. 2002. Experimental design. Fourth edition. New York, Wiley.
12 Juan, M.-Y. and C.-C. Chou. 2009. Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 14715. Food Microbiol. 27: 586-591.
13 Jung, S., J. H. Choe, B. Kim, H. Yun, Z. A. Kruk, and C. Jo. 2010. Effect of dietary mixture of gallic acid and linoleic acid on antioxidative potential and quality of breast meat from broilers. Meat Sci. 86: 520-526.   DOI   ScienceOn
14 Kim, M., M. C. Kim, J. S. Park, J. W. Kim, and J. O. Lee. 2001. The antioxidative effects of the water-soluble extracts of plants used as tea materials. Korean J. Food. Sci. Technol. 33: 12-18.
15 Kaushik, R., S. Saran, and J. Isar. 2006. Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus. J. Mol. Catal. B: Enzym. 40: 121-126.   DOI   ScienceOn