Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluation of Antiaging Activity on Lycium chinense Miller. Extracts

구기자 추출물의 항노화 활성 평가

  • Lee, Yong-Hwa (Department of Cosmetic biotechnology, Institute of Basic Sciences, Hoseo University) ;
  • Kim, ․Na-Yeon (Department of Cosmetic science, Hoseo University) ;
  • Jo, Il-young (Department of Cosmetic science, Hoseo University) ;
  • Yi, Yong-Sub (Department of Cosmetic biotechnology, Institute of Basic Sciences, Hoseo University)
  • 이용화 (호서대학교 화장품생명공학부, 기초과학연구소) ;
  • 김나연 (호서대학교 화장품과학과) ;
  • 조일영 (호서대학교 화장품과학과) ;
  • 이용섭 (호서대학교 화장품생명공학부, 기초과학연구소)
  • Received : 2022.02.08
  • Accepted : 2022.02.24
  • Published : 2022.02.28
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Abstract

The purpose of this study was to investigate the functionality of Lycium chinense Miller. as a functional cosmetic material. The total polyphenol content in the extract was 17.3 mg/mL, and as a result of confirming cytotoxicity through MTT assay before the enzyme experiment, it was confirmed that it did not affect cell growth at all concentrations below 1000 ㎍/mL. As a result of conducting DPPH radical scavenging tests as an antioxidant efficacy test, it showed 84.97% scavenging ability at a concentration of 1000 ㎍/mL, and showed very excellent antioxidant efficacy in SOD-like activity test with a result of 80.54% at 1000 ㎍/mL. The measured elastase inhibitory activity, the inhibition rate of 50.93% was observed at 1000 ㎍/mL, indicating that the extract of Lycium chinense Miller. was effective in inhibiting elastase. All these findings suggested that 70% ethanol extract of Lycium chinense Miller. was confirmed as a functional cosmetic material by confirming its antioxidant and anti-wrinkle efficacy.

본 연구는 구기자의 화장품 소재로서의 가능성을 검토하였다. 에탄올을 용매로 하여 얻어진 추출물 중 총 폴리페놀 함량은 17.3 mg/mL이었으며, 추출물의 세포 독성을 확인하기 위해 MTT assay결과 1000 ㎍/mL 이하의 농도에서 세포 생장에 영향을 미치지 않았다. 항산화 효능 실험으로 DPPH radical 소거 시험을 실시한 결과 1000 ㎍/mL의 농도에서 84.97%의 소거능을 나타내었고, SOD 유사 활성 실험 결과 1000 ㎍/mL에서 80.54%의 결과로 우수한 항산화 효능을 보였다. Elastase 저해 활성을 측정한 결과 1000 ㎍/mL에서 50.93%의 저해율을 나타내어 구기자 추출물이 elastase 저해에 효과가 있음을 확인할 수 있었다. 본 연구로부터 구기자 70% ethanol 추출물은 항산화와 주름 개선 효능을 확인함으로써 기능성 화장품 소재로의 활용 가능성을 확인하였다.

Keywords

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