• The Korean Journal of Food And Nutrition
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• v.33 no.6
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• pp.692-701
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• 2020

Centella asiatica (CA) has been widely used as herbal plants. It is a valuable resources. The aim of the present study was to evaluate physiological activities of solvent extracts from CA cultivated in Chungju, Korea (Good tiger care). After preparing water (cold-water, CA-WE; hot-water, CA-HWE) and EtOH extracts (50% EtOH, CA-50E; 70% EtOH, CA-70E), their total polyphenol, flavonoid, and triterpenoid contents, and anti-oxidant activities, and anti-inflammatory activities were examined and compared. CA-70E showed the most potent anti-oxidant activity based on ABTS radical scavenging activity and reducing ability assays, while CA-50E and CA-70E showed the highest DPPH radical scavenging activity. Among major triterpenoid glycosides present in CA, madecassoside and asiaticoside contents were found to be the highest in CA-70E, and madecassic acid and asiatic acid were the highest in CA-50E. In LPS-stimulated RAW 264.7 cells, CA-70E showed the highest TNF-α inhibitory activity, although CA-50E and CA-70E similarly inhibited nitric oxide production. Ethanol extracts significantly inhibited IL-6 and IL-8 production more than water extracts using TNF-α/IFN-γ-stimulated HaCaT cells, indicating its better inhibitory against skin inflammation. Therefore, Chungju-CA EtOH extract, especially 70% EtOH extract, has high physiologically active ingredients and potent anti-oxidant and anti-inflammatory activities, suggesting its industrial application as a functional material.

• Journal of Digital Convergence
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• v.19 no.7
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• pp.1-9
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• 2021

The core competencies such as technology have been rapidly developing with advent of the fourth industrial revolution. One of the representative technologies in the era of the 4th Industrial Revolution is artificial intelligence(AI). In particular, AI makes human life richer and more comfortable and has many positive aspects. The negative aspect is that AI is likely to replace the organizational members' job and has the ability to replace skills associated with it. These threats may increase the level of perception that organizational members may loss their job. Moreover, it may lead to a sense of anxiety. In addition, organizational members who perceive job uncertainty highly, the job substitution anxiety may be become highly. According to this, their job satisfaction can be lower. Overall, this research emphasized the negative aspects of AI in contrast to the positive aspects of the fourth industrial era. It is also emphasized that the organization should need to recognize such problems and seek solutions to reduce workers' anxiety. Finally, practical implications and research directions of future study were presented through the research results.

• Journal of Convergence for Information Technology
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• v.11 no.1
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• pp.143-155
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• 2021

The purpose of this study is to adopt qualitative research methods for alpine snowboard athlete to analyze their injury factors in depth from a psychological perspective. Therefore, in-depth interviews were conducted on four outstanding Alpine Snowboard athlete, and the results were as follows. Among the factors of the injury of Alpine snowboard athlete, the psychological point of view was derived from five factors: 'Trait Anxiety', 'Personality Characteristic', 'Expectancies of Significant Others, 'Discrepancy between ability and expectation' and 'Trauma Injuries'. The five psychological injury factors have expressed the raw data of the subject in a case-oriented manner, and empirical and field-oriented interview data will enable in-depth analysis of psychological factors related to injury to future Alpine snowboard athlete and coaches. It is expected to be used as a basis for injury prevention and countermeasures. All data can be used as a basis for positive effects on the quality of life of athletes and athletes in winter sports.

• The Journal of the Convergence on Culture Technology
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• v.8 no.1
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• pp.469-481
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• 2022

This study is for increasing evacuation safety by analyzing RSET(the required safe escape time) through the arrangement of personnel by floor and by room while evacuating in a Girls' High School Dormitory. For this study, PAPS(Physical Activity Promotion System) results that have not been studied so far were analyzed and reflected in evacuation simulations on the premise that individual student's physical strength can affect evacuation. Based on the PAPS results, four scenarios were applied. In addition, evacuation simulation using the pathfinder program was conducted in two situations: the evacuation route was assigned or not. Scenario 4 was the fastest at 168.5 seconds of RSET in assigning evacuation routes among scenarios. As a result of this study, the arrangement of students focusing on improving their academic ability and student life guidance excluding student physical strength has problem. In order to solve this problem, it is effective to place C group students(low grade on PAPS) on low floors and A group students(high grade on PAPS) on high floors and to assign evacuation routes in each room. In the future, the following ways need to be more studied. A study on how to increase evacuation safety through practical evacuation training, the way of assessing evacuation safety reflecting the lifestyle and physical strength of girls, the evacuation route assignment according to the fire occurrence point, and the method to secure evacuation routes in the event of a fire near stairs or entrances should be conducted.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.721-726
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• 2021

Plant cells have a totipotencial capacity, the ability of each cell to produce a new complete individual through development. By applying this, several technologies are being developed for widespread application of somatic embryogenesis by processing hormones in vitro as a method of propagation of plants. In order to use this technology, in Kalanchoe pinnata, a plant capable of asexual reproduction with more regular cell division, kinetin belonging to cytokinin and picloram among hormones belonging to auxin were added in combination and treated for 8 weeks, and then the typical performance was evaluated. As a result of our experiment, the rooting effect in leaf slices showed a 70% incidence rate at a picloram concentration of 0.1 mg/L. It has been proven that a concentration difference of 1:5-1:10 in the ratio of kinetin and picloram is effective. It is the experimental result that the effect of auxin is essential for the development of Kalanchoe roots. As for the effect of shooting, the incidence rate was 60% at the picloram concentration of 0.5 mg/L. The kinetin concentration from 0.5 and 1.0 mg/L and has a significant effect on development. It has been proven that the ratio of kinetin to picloram is effective with a concentration difference of 1:1-1:2. These results show that the combination of cytokinin and auxin is crucially important for shooting. It is thought that it can be the basis of a technology for inducing mass proliferation in vitro by inducing direct organogenesis with a combination of hormones.

• The Journal of the Korea Contents Association
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• v.22 no.1
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• pp.188-196
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• 2022

Although the use of kiosks is increasing recently, the digital divide for the elderly who uses them is not decreasing. We live in an environment where the use of kiosks is becoming a necessity rather than an option. In such an environment, the digital alienation of the elderly is becoming a problem directly related to the quality of life. Even if a kiosk is developed considering the elderly, the verification of its effectiveness is ambiguous, or in most cases, it depends on the designer's experiential ability rather than the consideration of usability. In this study, the usability of the kiosk was analyzed for the development of kiosk contents for the elderly. The metrics were defined as availability, usefulness, efficiency, attractiveness, and visibility. And the measurement method of the measurement index was developed, and the usability of the kiosk for the elderly was confirmed by performing usability evaluation. This is a method to verify whether the kiosk in the development process can support the elderly or whether the improved kiosk actually increases the usability of the elderly. As a result, it is expected to contribute to improving the accessibility of the kiosk.

• Mycobiology
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• v.50 no.1
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• pp.66-78
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• 2022

The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

• Journal of the Korean Society of Floral Art and Design
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• no.42
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• pp.63-96
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• 2020

The flower arrangement that human beings interact with the natural plants have characteristics according to their historical background and ethnicity and different aspects of culture related of the flower arrangements have been created by human inspiration and formative ability. The Korean flower arrangement is a product of civilization which originated in the natural environment of the four seasons. As background with the natural unity ideology, the Korean Flower Arrangement makes the life and philosophy of the naturalistic nature that flows in the mind of our nation to the relaxing beauty suited on cultures of ages by the Virtue, the Will, and the Vitality. The Literary Flower Arrangement is, based on the higher human personality and poetic sentiments as ideologies of our traditional oriental philosophy, the formative art made for aesthetic implications of philosophy by consisting plants' characters and tastes without expression of technical skills. In this study, focused on pine tree, in order to clarify that the flower arrangement of our spiritual culture is the Literary Flower Arrangement, make Identifying aesthetic characteristics through, the backgrounds and sources of being expressed by the Literary Flower Arrangement, poetic feelings, symbolism, metaphor, the formative form of flower arrangement, decoding of the flower arrangement genes, and the same time deduce the record of literary work and classify it each era, and establish the Literary Flower Arrangement theory which is the essence of Korean flower arrangement.

• The Journal of the Korea Contents Association
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• v.21 no.12
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• pp.47-56
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• 2021

The purpose of this study is to use network analysis to identify trends in university personality-related studies and provide implications for future research directions. For the purpose of this study, 194 papers related to personality of university students published in Korean scholarly journals. First, research began to be published in 2004, slightly increased in 2012, continued an upward curve from 2015, peaked in 2017, and is confirmed to be a downward trend. Second, the main keywords with the centrality analysis were 'society' and 'cultivation'. Third, keywords on the cognitive side and individual dimension of personality in the first period (2004 - 2010), social dimension and emotional side of personality in the second period (2011-2015), and social level and cognitive, emotional, and behavioral aspects of personality in the third period (2016-2020). Fourth, Topic 2 consisted of keywords of ability, life, interpersonal, satisfaction, and adaptation, and Topic 1 consisted of competence, morality, citizens, society, and practice. Fifth, Topic 4 alone in the first period, in the order of Topic 1 and Topic 2 in the second period, and in the order of Topic 2 and Topic 1 in the third period.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1105-1116
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• 2022

Recently, we reported the robust in vitro three-dimensional (3D) expansion of intestinal organoids derived from adult bovine (> 24 months) samples. The present study aimed to establish an in vitro 3D system for the cultivation of intestinal organoids derived from growing cattle (12 months old) for practical use as a potential alternative to in vivo systems for various purposes. However, very few studies on the functional characterization and 3D expansion of adult stem cells from livestock species compared to those from other species are available. In this study, intestinal crypts, including intestinal stem cells, from the small intestines (ileum and jejunum) of growing cattle were isolated and long-term 3D cultures were successfully established using a scaffold-based method. Furthermore, we generated an apical-out intestinal organoid derived from growing cattle. Interestingly, intestinal organoids derived from the ileum, but not the jejunum, could be expanded without losing the ability to recapitulate crypts, and these organoids specifically expressed several specific markers of intestinal stem cells and the intestinal epithelium. Furthermore, these organoids exhibited key functionality with regard to high permeability for compounds up to 4 kDa in size (e.g., fluorescein isothiocyanate [FITC]-dextran), indicating that apical-out intestinal organoids are better than other models. Collectively, these results indicate the establishment of growing cattle-derived intestinal organoids and subsequent generation of apical-out intestinal organoids. These organoids may be valuable tools and potential alternatives to in vivo systems for examining host-pathogen interactions involving epithelial cells, such as enteric virus infection and nutrient absorption, and may be used for various purposes.