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http://dx.doi.org/10.9799/ksfan.2020.33.6.692

Bioactive Compounds, Anti-Oxidant Activities and Anti-Inflammatory Activities of Solvent Extracts from Centella asiatica Cultured in Chungju  

Shin, Hyun-Young (Dept. of Integrated Biomedical and Life Science, Korea University)
Kim, Hoon (Skin Biotechnology Center, Kyung Hee University)
Jeong, Eun-Jin (Major in Food and Nutrition, Korea National University of Transportation)
Kim, Jeung-Eun (Major in Food and Nutrition, Korea National University of Transportation)
Lee, Kyeong-Haeng (Major in Food and Nutrition, Korea National University of Transportation)
Bae, Yun-Jeong (Major in Food and Nutrition, Korea National University of Transportation)
Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
Publication Information
The Korean Journal of Food And Nutrition / v.33, no.6, 2020 , pp. 692-701 More about this Journal
Abstract
Centella asiatica (CA) has been widely used as herbal plants. It is a valuable resources. The aim of the present study was to evaluate physiological activities of solvent extracts from CA cultivated in Chungju, Korea (Good tiger care). After preparing water (cold-water, CA-WE; hot-water, CA-HWE) and EtOH extracts (50% EtOH, CA-50E; 70% EtOH, CA-70E), their total polyphenol, flavonoid, and triterpenoid contents, and anti-oxidant activities, and anti-inflammatory activities were examined and compared. CA-70E showed the most potent anti-oxidant activity based on ABTS radical scavenging activity and reducing ability assays, while CA-50E and CA-70E showed the highest DPPH radical scavenging activity. Among major triterpenoid glycosides present in CA, madecassoside and asiaticoside contents were found to be the highest in CA-70E, and madecassic acid and asiatic acid were the highest in CA-50E. In LPS-stimulated RAW 264.7 cells, CA-70E showed the highest TNF-α inhibitory activity, although CA-50E and CA-70E similarly inhibited nitric oxide production. Ethanol extracts significantly inhibited IL-6 and IL-8 production more than water extracts using TNF-α/IFN-γ-stimulated HaCaT cells, indicating its better inhibitory against skin inflammation. Therefore, Chungju-CA EtOH extract, especially 70% EtOH extract, has high physiologically active ingredients and potent anti-oxidant and anti-inflammatory activities, suggesting its industrial application as a functional material.
Keywords
Centella asiatica; solvent extract; anti-oxidant; anti-inflammation; pentacyclic terpenoid;
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Times Cited By KSCI : 8  (Citation Analysis)
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