• Journal of the Korean GEO-environmental Society
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• v.10 no.5
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• pp.49-57
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• 2009

In this study, one of the complicated geotechnical problem, compression index was predicted by a artificial neural network method of Levenberg-Marquardt (LM) algorithm. Predicted values were compared and evaluated by the results of the Back Propagation (BP) method, which is used extensively in geotechnical engineering. Also two different results were compared with experimental values estimated by verified experimental methods in order to evaluate the accuracy of each method. The results from experimental method generally showed higher error than the results of both artificial neural network method. The predicted compression index by LM algorithm showed better comprehensive results than BP algorithm in terms of convergence, but accuracy was similar each other.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.12B
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• pp.1124-1134
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• 2003

The recent intensification of competition in the LM market has become a major issue. In this research effort, a study is made to determine the expected social benefits that can be realized from the increase of competition in the LM market through CPS. Results from this study show that consumer benefits up to 144.8 billion won can be created through the boost in competition from CPS. However, a reduction of 207.9-438.7 billion won in revenues is expected for service providers. Depending on the existing service providers, revenues may fall by at least 264.4 billion won and up to 486.2 billion won. For newcomers to the market 30.2-89.1 billion won will be created.

• Journal of Animal Science and Technology
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• v.62 no.6
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• pp.864-874
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• 2020

Lactic acid bacteria are well-known probiotics, conferring several health benefits. In this study, we isolated lactobacilli from human breast milk and identified Lactobacillus reuteri LM1071 (RR-LM1071) using 16S rDNA sequencing. We tested the hemolytic activity, biogenic amine production, and antibiotic susceptibility of this strain to assess its safety. RR-LM1071 was found to be negative for hemolytic activity and biogenic amine production, as well as was measured in susceptible level for antibiotics in the minimal inhibitory concentration (MIC) test. The adhesive properties of RR-LM1071 were higher than those of LGG in HT-29 cells, and showed a greater hydrophobicity than LGG in hexadecane solvent. Under inflammatory conditions, RR-LM1071 suppressed the mRNA expression of IL-6, TNF-α, and IL-4 produced in IL-1β-induced HT-29 cells. Our results suggest that RR-LM1071 is a safe and valuable probiotic that can be used for the treatment of inflammatory bowel disease.

• Food Science of Animal Resources
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• v.44 no.4
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• pp.951-965
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• 2024

Lactiplantibacillus plantarum is a valuable potential probiotic species with various proven health-beneficial effects. L. plantarum LM1001 strain was selected among ten strains of L. plantarum based on proteolytic activity on whey proteins. L. plantarum LM1001 produced higher concentrations of total free amino acids and branched-chain amino acids (Ile, Leu, and Val) than other L. plantarum strains. Treatment of C2C12 myotubes with whey protein culture supernatant (1%, 2% and 3%, v/v) using L. plantarum LM1001 significantly increased the expression of myogenic regulatory factors, such as Myf-5, MyoD, and myogenin, reflecting the promotion of myotubes formation (p<0.05). L. plantarum LM1001 displayed β-galactosidase activity but did not produce harmful β-glucuronidase. Thus, the intake of whey protein together with L. plantarum LM1001 has the potential to aid protein digestion and utilization.

• Journal of Food Hygiene and Safety
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• v.22 no.4
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• pp.332-337
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• 2007

This study was done to determine the adherence rate of Listeria monocytogenes(LM) from pork meat to pork meat, conveyer belt and stainless steel, and cutting board at $10^{\circ}C$ for given times (2, 4, 24 hours). The adherence rates of ATCC and wild type LM from pork meat to red meat or fat meat after 2 hour contact time were approximately 65-79%, but 100% adherence rates of both LM after 6 hours were observed. The adherence rate of ATCC LM from pork meat to conveyer belt and stainless steel after 24 hours was 0.15%, while the adherence rate of wild type LM was rapidly increased from 4.34 after 2 hours to 28.6% after 24 hours. Also, The adherence rate of ATCC LM from pork meat to stainless steel after 24 hours ranged from 2.63% after 2 hours to 0.39% after 24 hours, while the adherence rate of wild type LM ranged from 0% after 2 hours to 24.4% after 24 hours. In the meantime, no adherence rate of both LM from pork meat to cutting board was observed. This results suggest that the adherence rate of LM from pork meat to pork meat or food contact surfaces was significantly affected by contact time and wild type LM showed much higher adherence rate to pork meat or food contact surfaces than ATCC LM.

• Journal of the Korean Statistical Society
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• v.28 no.4
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• pp.489-501
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• 1999

In this paper, we consider the panel data regression model in which the disturbances have nested error component. We derive a Lagrange Multiplier(LM) test which is jointly testing for the presence of random individual effects and nested effects under the normality assumption of the disturbances. This test extends the earlier work of Breusch and Pagan(1980) and Baltagi and Li(1991). Further, it is shown that this LM test has the same asymptotic distribution without normality assumption of the disturbances.

• Radiation Oncology Journal
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• v.17 no.4
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• pp.299-306
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• 1999

Purpose : The human genetic disorder ataxia-telangiectasia (AT) is a multisystem disease characterized by extreme radiosensitivity. The recent identification of the gene mutated in AT, ATM, and the demonstration that it encodes a homologous domain of phosphatidylinositol 3-kinase (PI3-K), the catalytic subunit of an enzyme involved in transmitting signals from the cell surface to the nucleus, provide support for a role of this gene in signal transduction. Although ionizing radiation was known to induce c-fos transcription, nothing is known about how ATM or PKCI mediated signal transduction pathway modulates the c-fos gene transcription and gene expression. Here we have studied the effect of PKCI on radiation sensitivity and c-fos transcription in normal and AT cells. Materials and Methods: Normal (LM217) and AT (AT5BIVA) cells were transfected with PKCI expression plasmid and the overexpression and integration of PKCI was evaluated by northern blotting and polymerase chain reaction, respectively. 5 Gy of radiation was exposed to LM and AT cells transfected with PKCI expression plasmid and cells were harvested 48 hours after radiation and investigated apoptosis with TUNEL method. The c-fos transcription activity was studied by performing CAT assay of reporter gene after transfection of c-fos CAT plasmid into AT and LM cells. Results: Our results demonstrate for the first time a role of PKCI on the radiation sensitivity and c-fos expression in LM and AT cells. PKCI increased radiation induced apoptosis in LM cells but reduced apoptosis in AT cells. The basal c-fos transcription activity is 70 times lower in AT cells than that in LM cells. The c-fos transcription activity was repressed by overexpression of PKCI in LM cells but not in AT cells. After induction of c-fos by Ras protein, overexpression of PKCI repressed c-fos transcription in LM cells but not in AT cells Conclusion: Overexpression of PKCI increased radiation sensitivity and repressed c-fos transcription in LM cells but not in AT cells. The results may be a. reason of increased radiation sensitivity of AT cells. PKCI may be involved in an ionizing radiation induced signal transduction pathway responsible for radiation sensitivity and c-fos transcription. The data also provided evidence for novel transcriptional difference between LM and AT cells.

• Journal of Life Science
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• v.28 no.1
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• pp.17-25
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• 2018

In this study, we examined the efficacy of the immune regulation of ${\beta}$-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on atopic dermatitis models. The oral administration of ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 on mice significantly decreased the amount of scratching, leakage to evans blue, and concentrations of serum immunoglobulin E (IgE) and histamine compared with the atopic dermatitis - induced group. When atopic dermatitis was induced, the transcription factors (GATA-3, retinoic acid-related orphan receptor ${\gamma}$ T [$ROR{\gamma}T$]) and cytokines (interleukin-4 [IL-4], IL-17) of Th2 and Th17 cells were overexpressed at the transcriptional level, and they significantly decreased with oral administration of ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004. In addition, ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 were shown to modulate the immune balance by increasing the expression of Th1 and Treg transcription (T-bet, forkhead box p3 [Foxp3]) and cytokines (interferon-${\gamma}$ [IFN-${\gamma}$], transforming growth factor-${\beta}$ [TGF-${\beta}$]). Galectin-9 and filaggrin were significantly lower in the atopic dermatitis - induced group and significantly higher in the ${\beta}$-1,3/1,6-glucan-treated group. In contrast, thymic stromal lymphopoietin (TSLP) was highest in the atopic dermatitis-induced group, while mice that were orally administered ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 showed similar TSLP levels to the control group. These results indicate that ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 have immunomodulatory effects and atopic dermatitis improvement effects in an animal model of atopic dermatitis. Therefore, it is expected that ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 can be used as natural materials in the treatment of atopic dermatitis.

• Korean Journal of Poultry Science
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• v.27 no.3
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• pp.267-277
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• 2000

These studies were conducted to investigate the effect of dietary supplemental lincomycin at different food waste supplements on performance and blood components of broiler chicks. Diets were based corn, soybean meal and contained 3,100㎉/kg ME, 21% CP for the rest two weeks. Three levels of dietary lincomycin(LM ; 0, 2.2, 4.4 mg/kg) and food waste(FW : 0, 7.5, 15%) were added for both experiments. Weight gain, feed intake and feed conversion ratio(FCR) were measured for five weeks. Blood components, liver weight and abdominal fat ratio to body weight were examined at the end of experiment. Metabolizable energy of food waste used in formulating diet prior to feeding trials were measured 2,504(Expt 1) and 2,734㎉/kg(Expt 2), respectively. In Expt 1, weight gain of birds fed 4.4mg/kg LM was significantly higher than that of no LM treatment(P〈0.05). It also tended to increase in 7.5% food waste treatment compared to that of control but was not significantly different. FCR of chicks fed 2.2mg/kg LM supplement was significantly improved compared to that of other treatments. However, chicks fed food waste supplements had higher FCR than control as a dietary FW level increased(P〈0.05). There were no significance in blood components of all treatments. Liver to body weight ratio of birds fed 7.5% FW supplements decreased significantly(P〈0.05), but was no consistency in LM treatments. In Expt 2, weight gain of chicks fed 15% FW was not significantly different from the control and 7.5% FW. It tended to increase as dietary supplemental LM increased. It also increased in no LM treatment with 15% FW compared to that of control. However, the birds fed 2.2mg/kg LM with 15% FW exhibited higher weight gain than dther treatments. As FW supplement increased, the feed intake increased significantly(P〈0.05). There were no significance in blood components except serum calcium. Weights of liver were not affected by dietary FW or LM. The results of these experiments indicated that chicks fed 4.4mg/kg LM supplements maximized growth rate of all treatments and improved FCR in 2.2mg/kg LM.

• Journal of fish pathology
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• v.35 no.2
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• pp.195-203
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• 2022

This study was performed to delineate pharmacokinetic residue characteristics of lincomycin (LM) in olive flounder at low water temperature (13℃), and to examine whether LM is effective against main Gram-negative pathogens. It was observed that the times for residue declined below the maximal residue limit (0.1 mg/kg) in the muscle was, respectively, 32, 33 and 55 days following 10, 20 and 40 mg/kg intramuscular LM injections. The in vitro MIC value of LM against Edwardsiella piscida was higher than 256 ㎍/mL and against Vibrio harveyi 32 ㎍/mL when examined with several clinical isolates. These results suggest that the withdrawal time of LM should be more than 30 days in consideration of the 10 mg/kg employed as the regular clinical dose. It was also found that LM administered to treat streptococosis in olive flounder is not likely to be effective against some pathogenic Gram-negative bacteria.