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http://dx.doi.org/10.5352/JLS.2018.28.1.17

Immunomodulatory Effects of β-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on Atopic Dermatitis Models  

Kim, In Sung (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Kim, Sung Hak (Department of Animal Science, Chonnam National University)
Kim, Jeong A (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Yu, Da Yoon (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Kim, Gwang Il (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Park, Dong-Chan (Glucan Corporation)
Lim, Jong Min (Glucan Corporation)
Lee, Sang Suk (Department of Animal Science and Technology, Sunchon National University)
Choi, In Soon (Departmnet of Life Science, Silla University)
Cho, Kwang Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.28, no.1, 2018 , pp. 17-25 More about this Journal
Abstract
In this study, we examined the efficacy of the immune regulation of ${\beta}$-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on atopic dermatitis models. The oral administration of ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 on mice significantly decreased the amount of scratching, leakage to evans blue, and concentrations of serum immunoglobulin E (IgE) and histamine compared with the atopic dermatitis - induced group. When atopic dermatitis was induced, the transcription factors (GATA-3, retinoic acid-related orphan receptor ${\gamma}$ T [$ROR{\gamma}T$]) and cytokines (interleukin-4 [IL-4], IL-17) of Th2 and Th17 cells were overexpressed at the transcriptional level, and they significantly decreased with oral administration of ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004. In addition, ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 were shown to modulate the immune balance by increasing the expression of Th1 and Treg transcription (T-bet, forkhead box p3 [Foxp3]) and cytokines (interferon-${\gamma}$ [IFN-${\gamma}$], transforming growth factor-${\beta}$ [TGF-${\beta}$]). Galectin-9 and filaggrin were significantly lower in the atopic dermatitis - induced group and significantly higher in the ${\beta}$-1,3/1,6-glucan-treated group. In contrast, thymic stromal lymphopoietin (TSLP) was highest in the atopic dermatitis-induced group, while mice that were orally administered ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 showed similar TSLP levels to the control group. These results indicate that ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 have immunomodulatory effects and atopic dermatitis improvement effects in an animal model of atopic dermatitis. Therefore, it is expected that ${\beta}$-1,3/1,6-glucan and L. plantarum LM1004 can be used as natural materials in the treatment of atopic dermatitis.
Keywords
Atopic dermatitis; ${\beta}$-Glucan; immunomodulatory; Lactobacillus plantrum LM1004;
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