• Journal of Applied Biological Chemistry
• /
• 제63권3호
• /
• pp.181-188
• /
• 2020

In this study, Tongue fern (Pyrrosia lingua) plants that have been used traditionally as medicines. Their traditional medicinal uses, regions where indigenous people use the plants, parts of the plants used as medicines. This study was designed to assess the antioxidant and inhibition activities of extracts from P. lingua. In the P. lingua extracts was measured ethanol activity, 80.0% ethanol was high activity. The antioxidant activity was measured in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), assays. DPPH and ABTS radical in this experiment, solid and phenolic of extract were tested, but only an average concentration of 100 ㎍/mL was used. However, the phenolic extract is shown phenolic activity reached a peak. Also, phenolic extracts ware reached peak water and ethanol extracts. As a result, using the phenolic extracts did other antioxidant assays such as DPPH, ABTS, protection factor, and thiobarbituric acid reactive substances at 50-200 ㎍/mL concentrations. The activity of elastase and collagenase, inhibiting their activities may retard skin aging. α-Glucosidase and α-amylase, inhibitors need to be explored for the benefit of postprandial hyperglycemia in diabetic patients. Activities of tyrosinase, hyaluronidase and xanthine oxidase inhibitors of these enzymes are increasingly important ingredients in cosmetics and medications to protect the skin against hyperpigmentation and skin aging. Inhibition effects were investigated using the P. lingua extracts at 50-200 ㎍/mL concentrations. The expression levels of enzyme inhibitions activities were decrease in dependent-concentrations manner when P. lingua extracts were treated.

• 대한환경공학회지
• /
• 제35권10호
• /
• pp.687-693
• /
• 2013

다양한 난개발과 해안도로, 방파제 등의 인공 구조물 설치로 인한 해안 침식과 해안선 파괴 등으로 해안사구가 크게 훼손되고 있다. 이에 본 연구에서는 해안사구에서 서식하고 있는 토착식물의 근권으로부터 식물 성장 촉진 능력이 있으면서 동시에 염분에 강한 내성을 가지는 근권세균의 library를 구축하였고, 이들 균주를 대상으로 식물 성장 촉진 능력을 평가하였다. 또한, 내염성 식물을 대상으로 사구 토양에서의 성장률에 근권세균이 미치는 영향을 평가한 후, 훼손된 사구의 복원에 가장 유용한 미생물을 선별하고 분리된 근권세균이 식물 성장에 미치는 영향을 평가하였다. 실험은 선정된 균주와 갯기름나물과 줄무늬갈대를 해안사구에 식재한 후 성장에 미치는 영향을 평가하였다. 그 결과, MH1RS1는 (IAA) production, siderophores, 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) 그리고 염분내성을 가지고 있다. 갯기름나물의 경우는 줄기의 길이와 뿌리의 무게는 크게 향상되었다. 특히, 줄기의 생체와 뿌리 무게는 control과 비교했을 때 25% 성장이 향상되었다. 줄무늬 갈대는 Bacillus aerius MH1RS1 에 의해 초장은 18%와 뿌리의 생체 중은 20%로 크게 향상 되었다.

• 생태와환경
• /
• 제56권4호
• /
• pp.363-374
• /
• 2023

우리나라를 대표하는 생물종 풍부도를 도출하는 데에는 상대적으로 짧은 생물종 현장 조사 역사와 여러 기관에서 분야별로 수집한 조사 자료가 산재되어 있다는 한계가 있다. 본 연구에서는 환경부 산하기관들이 보유하고 있는 관속식물 조사 데이터를 취합하여 100년 이상의 시계열 데이터세트를 구축하였다. 자료 통합은 종명, 위치, 시간(연도) 등 최소한의 기준을 적용해 데이터 검증 및 보정 과정을 거쳤다. 통합한 식물종 자료에 따르면 국내 식물종에 대한 체계적인 수집은 2000년 이후에 주로 이루어졌으며, 이러한 조사를 통해 발견된 식물종의 수는 최근 수렴하는 경향을 보이고 있었다. 이는 우리나라에서 국가 차원의 생물종 다양성을 도출할 수 있는 조건이 충분히 갖추어지고 있음을 시사한다. Chao 2 방법을 이용하여 예측한 1951년부터 70년간의 토착식물의 종 풍부도는 3,182.6으로 추정되었다. 이 추정치를 얻기 위해서는 최소 7년의 누적 기간이 필요하다. 본 연구의 식물종 풍부도는 향후 우리나라의 종 풍부도 변화를 연구하는데 기준이 될 수 있다. 또한 사용한 종 풍부도 추정 방법과 통합자료는 지자체 수준 등의 지역 생물다양성을 도출하는 데에도 적용할 수 있을 것으로 생각한다.

• 한국조경학회지
• /
• 제27권3호
• /
• pp.1-17
• /
• 1999

The purpose of this study is to evaluate the creation techniques of artificial wetland, one of biotopes developed to promote biodiversity in urban areas, and to look for improvement steps. Specifically, artificial wetland creation techniques were categorized into living environment and living creature classification. Being living conditions for creations, habitat environment was reviewed with a focus on water and soil environments. Living creatures were classified into plants, insects, fish, and birds. The evaluation of creation techniques was done in post-construction evaluation while considering the creation of habitats for living creatures. Intervention by users, changes in living environment and living species, and relevance of creation techniques were reviewed. Key results of this study are as follows. (1) Water environment for the living environment of creatures provides a suitable environment conditions for the living of creatures through a process easing the use of piped water. Various water depths and embankment appear to have a positive impact on the living of aquatic life. In particular, embankment covered in soil naturally played an important role as a place for the activities of aquatic insects and young fish as well as the growth of aquatic plants. (2) Various aquatic and ground plants to promote insect-diversity, shallow water, and old-tree logs had contributed greatly in increasing the types and number of insects. Aquatic insects. Aquatic insects were seen much particularly in areas where aquatic plants are rich but water is shallow than any other areas. (3) A space piled with stone to provide habitats for fish was not much used. However, it was observed that fish used embankment built with natural stones and embankment using logs in areas where water is deep. In addition, it was confirmed that 1,500 fish that had been released propagated using various depths and places for birth. (4) It was analyzed that techniques (creation of island, log setting, and creation of man-made bird nests) to provide habitats and to attract birds are not serving their roles. In such a case, it is believed that species had not increased due to the smallness as well as isolated features of the area. Based on theoretical review, they are judged to be areas that are likely to be used when a greater variety of birds is introduced. It is judged that attracting and keeping more birds at the site, such spaces need to be linked systematically in the future in terms of building eco-network while ensuring an adequate living areas. (5) In the study areas, users intervened greatly. As a result, a blockage was created preventing the normal growth of plants and non-indigenous plants were introduced. In order to limit the intervention by users, setting enough buffer zones, and environment education programs were urgently required. D/H=1>Hyangkyo> houses on the river>temples>lecture halls. D/H ratio of the backside areas is as follows. D/H=1>Hyangkyo>houses on the river>lecture halls. 4. Inner garden were planted deciduous than evergreen trees with Lagerstroemia indica. Enclosed dominant trees were planted by Pinus densiflora, Querces seuata. construct GEM strain, and examined for the expression and functional stability in microcosms.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제6권4호
• /
• pp.3-11
• /
• 2001

TNT를 제거하기 위한 식물종을 선정하기 위해 8종의 대표적인 야초류를 이용하여 TNT 식물독성 임계점을 측정하였다. 독성 임계점은 TNT 농도변화에 대한 발아율, 유식물의 뿌리와 지상부의 생장율, 생체량 및 수분 흡수 능력 등을 비교하여 결정하였다. TNT가 식물에게 미치는 독성효과는 오염물의 농도와 식물종에 따라 다르게 나타났다. 60-80mgTNT/L 농도는 어저귀, 식용피, 자귀풀 종자의 발아율에 영향을 미치지 못하였다. 발아 후 14일동안 어저귀, 식용피, 자귀풀의 뿌리 생장에 있어 식물 독성 임계점(50%) 농도는 5-40 mgTNT/L이며 지상부 생장은 50-73mgTNT/L, 생체량은 68-99mgTNT/L이다. 뿌리와 지상부의 길이, 생체량은 TNT농도가 증가함에 따라 감소하였으며 수분 흡수량도 감소하였다. 야초류의 서로 다른 독성 임계점을 비교해 보면 뿌리 길이 > 지상부 길이 > 생체량 > 발아율 순으로 TNT에 대해 민감하게 반응하였다. 실험 결과, 8종의 식물 중에서 어저귀와 자귀풀은 TNT에 대해 내성을 지닌 종으로 판명되었다.

• 대한예방한의학회지
• /
• 제20권2호
• /
• pp.77-86
• /
• 2016

Objectives : This study aims to investigate the current situation of domestic patents in Korea on natural products and to provide directions for developing and applying herbal medicinal products by in-depth analyses. Methods : Combinations of words, such as "A61K" or "A61P", "herb" or "Korean medicinal herb" or "Korean medicine" or "traditional" or "natural" or "$botanic^*$" or "China", and "medicine" or "treatment" or "prevention" or "improvement", were used to search patents in the World Intellectual Property Service (WIPS) database. Three methods were applied to analyze natural products patents. First, the number of patent registrations was sorted by year. Second, the assignees were analyzed by count and country of origin. Finally, cluster map analysis was conducted to explore frequently emerging words in natural products and the relationship amongst those words to treat corresponding diseases/technologies. Results : Results showed that, first, the total number of patents had been increasing. Among the patents, 76.4% were registered by domestic institutes/companies, and 23.6% by foreign institutes/companies. Second, USA, Japan, and China possess a considerable number of Korean patents and, therefore, domestic institutes/companies can seek joint technological development opportunities with their counterparts from those countries in the future. Finally, a total of four clusters were identified by cluster map analysis. Each of the clusters includes natural products related to diseases involving skin, aging, and blood sugar, as well as adult diseases. Conclusions : In this study, natural products patents registered in the Korean Intellectual Property Office were analyzed. The analyses results showed the kinds of natural products that had been employed for the treatment of certain diseases. However, natural products included in existing patents were minimal given that 4,174 species of indigenous plants are found in Korea. Thus, Korean institutes/companies should utilize unexplored plants to develop more value-added drugs.

• 생약학회지
• /
• 제34권2호통권133호
• /
• pp.123-127
• /
• 2003

Atrartylodis Rhizoma(蒼朮)'s origin plants are Atratylodes lancea and A. Chinensis in Chinese, Japanese and Korean pharmacopoeia. A. Japonica is only indigenous in Korea, it is actually used as Atractylodis Rhizoma in Korean market. A. lancea is used in Hunan province, China and A. Chinensis is used in Hubei province, China. It is impossible to distinguish with species differency as macro- and micro-morphology. We tried to distinguish with species differency by HPLC and GC-Mass spectra. Atractylone(mw. 216) which is a marker compound in Atractylodis Rhizoma Alba(白朮) was detected in A. japonica. Atractylodin (mw.182) was detected in A. lancea and two eudesmadien derivatives (mw. 204) were detected in A. chinensis. HPLC chromatogram showed the same patterns. As a result, we propose that A. japonica will be added as Atractylodis Rhizoma (蒼朮)'s origin plant in Korean Pharmacopoeia. Atractylodis Rhizoma Alba(白朮)'s origin plants are A. macrocephala in China, and A. Japonica and A. ovata in Korea and Japan. In GC-Mass analysis, all samples showed same patterns and the main compound was atractylone.

• 농촌계획
• /
• 제21권4호
• /
• pp.157-165
• /
• 2015

The purpose of this study is on identifying charactreistics and management direction of complementary pond of Dalmaji village in Gangjin. Accordingly, this village was indicated as Experience and Resort village by Korean government. This study describes the construction background and characteristics and identifies species diversity and characteristics through field survey. For this, literature and field survey was performed. In field survey, vegetational survey was performed in Aug. of 2015. D(Dominant degree) and S(Sociability degree) was measured by Brown-Blanquet's method. The answer was searched from Myungsanron(明山論), a textbook of Fengsui(風水) in Joseon dynasty. Of these causes, it was considered that the representative one is a protection of fire and infectious disease because the Wolgak mountain is a fire mountain(火山) as a stone mountain in Fengsui. The results demonstrate decline of aquatic ecosystem because of neglect management. The total number of species was 24. Of the species, Zelkova serrata is a natural vegetation of this complementary forest. This is nominated as Lagerstroemia indica and Camellia japonica community. Species composition demonstrate the characteristics of Korean southern region as mixed forest of evergreen trees and deciduous ones which are indigenous plants. It was considered Juniperus chinensis KaizukaVariega in central round island was planted in Japanese colonial period and it have to be removed. It was considered that name of moon pond and moon island etc. projects the mind of village ancestors which are going to reach an ideal condition of moon. And management of quantity and depth of pond water was critical for restoration of disappearing lotus from related literature. Periodic management was needed for ecological structure and function improvement because of ecological disturbance by the invasive plants like Humulus japonicus.

• Natural Product Sciences
• /
• 제12권1호
• /
• pp.1-7
• /
• 2006

We have screened the cytoprotective effect on ${\gamma}-ray$ radiation induced oxidative stress from forty one Korean plant extracts. Carpinus laxiflora (caulis), Quercus salicina (caulis), and Castanopsis cuspidata (caulis) were found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). As a result, extracts of three plants reduced cell death of Chinese hamster lung fibroblast (V79-4) cells induced by $H_2O_2$ treatment. In addition, these extracts protected cell death of V79-4 cells damaged by ${\gamma}-ray$ radiation. In addition, these extracts scavenged ROS generated by radiation. Taken together, the results suggest that Carpinus laxiflora, Quercus salicina, and Castanopsis cuspidata protect V79-4 cells against oxidative damage by radiation through scavenging ROS.

• Journal of Radiation Protection and Research
• /
• 제31권3호
• /
• pp.115-122
• /
• 2006

We have screened the cytoprotective effect on $\gamma$-ray radiation induced oxidative stress from eighteen Korean plant extracts. Querus salicina, Clerodendron trichotomum, Lamium amplexicaule, Lozoste lancifolia and Malus baccata were found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). As a result, extracts of these plants reduced cell death of Chinese hamster lung fibroblast (V79-4) cells induced by $H_2O_2$ treatment. In addition, these extracts protected cell death of V79-4 cells damaged by $\gamma$-ray radiation. In addition, these extracts scavenged ROS generated by radiation. Taken together, the results suggest that Quercus salicina, Clerodendron trichotomum, Lamium amplexicaule, Lozoste lancifolia and Malus baccata protect V79-4 cells against oxidative damage by radiation through scavenging ROS.