• BMB Reports
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• v.51 no.7
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• pp.338-343
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• 2018

Transcription termination factor-1 (TTF-I) is an RNA polymerase 1-mediated transcription terminator and consisting of a C-terminal DNA-binding domain, central domain, and N-terminal regulatory domain. This protein binds to a so-called 'Sal box' composed of an 11-base pair motif. The interaction of TTF-I with the 'Sal box' is important for many cellular events, including efficient termination of RNA polymerase-1 activity involved in pre-rRNA synthesis and formation of a chromatin loop. To further understand the role of TTF-I in human immunodeficiency virus (HIV)-I virus production, we generated various TTF-I mutant forms. Through a series of studies of the over-expression of TTF-I and its derivatives along with co-transfection with either proviral DNA or HIV-I long terminal repeat (LTR)-driven reporter vectors, we determined that wild-type TTF-I downregulates HIV-I LTR activity and virus production, while the TTF-I Myb-like domain alone upregulated virus production, suggesting that wild-type TTF-I inhibits virus production and trans-activation of the LTR sequence; the Myb-like domain of TTF-I increased virus production and trans-activated LTR activity.

• Biomolecules & Therapeutics
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• v.22 no.5
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• pp.390-399
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• 2014

Chalcones (1,3-diaryl-2-propen-1-ones), a flavonoid subfamily, are widely known for their anti-inflammatory properties. Propenone moiety in chalcones is known to play an important role in generating biological responses by chalcones. In the present study, we synthesized chalcone derivatives structurally modified in propenone moiety and examined inhibitory effect on nitric oxide (NO) production and its potential mechanisms. Among the chalcone derivatives used for this study, TI-I-174 (3-(2-Hydroxyphenyl)-1-(thiophen-3-yl)prop-2-en-1-one) most potently inhibited lipopolysaccharide (LPS)-stimulated nitrite production in RAW 264.7 macrophages. TI-I-174 treatment also markedly inhibited inducible nitric oxide synthase (iNOS) expression. However, TI-I-174 did not significantly affect production of IL-6, cyclooxygenase-2 (COX-2) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), implying that TI-I-174 inhibits production of inflammatory mediators in a selective manner. Treatment of macrophages with TI-I-174 significantly inhibited transcriptional activity of activator protein-1 (AP-1) as determined by luciferase reporter gene assay, whereas nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activity was not affected by TI-I-1744. In addition, TI-I-174 significantly inhibited activation of c-Jun-N-Terminal kinase (JNK) without affecting ERK1/2 and p38MAPK, indicating that down-regulation of iNOS gene expression by TI-I-174 is mainly attributed by blockade of JNK/AP-1 activation. We also demonstrated that TI-I-174 treatment led to an increase in heme oxygenase-1 (HO-1) expression both at mRNA and protein level. Transfection of siRNA targeting HO-1 reversed TI-I-174-mediated inhibition of nitrite production. Taken together, these results indicate that TI-I-174 suppresses NO production in LPS-stimulated RAW 264.7 macrophages via induction of HO-1 and blockade of AP-1 activation.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.14 no.3
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• pp.223-234
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• 2016

This study aimed to the adsorption-removal of high- radioactive iodide (I) contained in the initially generated high-radioactive seawater waste (HSW), with the use of AgX (Ag-impregnated X zeolite). Adsorption of I by AgX (hereafter denoted as AgX-I adsorption) was increased by increasing the Ag-impregnated concentration in AgX, and its concentration was suitable at about 30 wt%. Because of AgCl precipitation by chloride ions contained in seawater waste, the leaching yields of Ag from AgX (Ag-impregnated concentration : about 30~35 wt%) was less than those in distilled water (< 1 mg/L). AgX-I adsorption was above 99% in the initial iodide concentration ($C_i$) of 0.01~10 mg/L at m/V (ratio of weight of adsorbent to solution volume)=2.5 g/L. This shows that efficient removal of I is possible. AgX-I adsorption was found to be more effective in distilled water than in seawater waste, and the influence of solution temperature was insignificant. Ag-I adsorption was better described by a Freundlich isotherm rather than a Langmuir isotherm. AgX-I adsorption kinetics can be expressed by a pseudo-second order rate equation. The adsorption rate constants ($k_2$) decreased by increasing $C_i$, and conversely increased by increasing the ratio of m/V and the solution temperature. This time, the activation energy of AgX-I adsorption was about 6.3 kJ/mol. This suggests that AgX-I adsorption is dominated by physical adsorption with weaker bonds. The evaluation of thermodynamic parameters (a negative Gibbs free energy and a positive Enthalpy) indicates that AgX-I adsorption is a spontaneous reaction (forward reaction), and an endothermic reaction indicating that higher temperatures are favored.

• Journal of the Korean Society of Industry Convergence
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• v.10 no.3
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• pp.165-169
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• 2007

Prediction of fatigue duration is attainable from the analysis of the growth rate of the fatigue crack, and the property of the fatigue crack growth is determined by the calculation of the stress intensity factor. And the evaluation of the stress intensity factor, K comes from the stress analysis of the vicinity of crack tip of the continuum. This study describes a simple method to decide the stress intensity factor for the small crack at the sharp edge notches. The proposed method is based on the similarities between elastic stress fields of the notch tip described by two parameters, the stress concentration factor K, the radius of arc of the notch. And it is applicable to the analysis of the semi-elliptical penetration cracks and the edge notches.

• Proceedings of the KIEE Conference
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• 2003.07d
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• pp.2271-2273
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• 2003

Control Rod Control System(CRCS) is to control nuclear reaction of reactor by moving Control Rod Drive Mechanism(CRDM) with speed and direction signal from Reactor Regulating System(RRS). CRCS is made up of two parts : control cabinet and power cabinet. And this paper presents mainly power cabinet design for system reliability. To increase reliability of power cabinet, controller, power supply and communication line arc doubly designed and supervision and diagnosis function are applied.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.171-175
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• 2004

Insulin-like growth factor-I (IGF-I) rich fraction, which was obtained molecules ranged between 30 kDa and 1 kDa, was fractionated by ultrafiltration from bovine colostral whey with 30 kDa and 1 kDa membrane. IGF-I included in fractionated IGF-I rich fraction was confirmed by SDS-PAGE and western blotting and then the quantity of IGF-I was measured by ELISA. IGF-I concentration in IGF-I rich fraction was 10ng/mg protein. Effect of IGF-I rich fraction on in vitro proliferation of several cells was tested. IEC-6 cell proliferation rate was increased 60％. 53％, 30％, and 20％ at l0ng, 1ng, 0.1ng and IGF-I of IGF-I, respectively, compared to control group which was not supplemented by IGF-I rich fraction. IGF-I rich fraction stimulated in vitro proliferation of IEC-6 cell in a dose dependent manner by increasing cell number. Detroit 551 cell proliferation was enhanced 56％ and 26％ at 10ng and 1ng level of IGF-I, respectively, compared to control group. EL-4 cell and L6 cell proliferation was increased 53％ and 46％ at 10ng of IGF-I, respectively, compared to control group.

• Journal of applied mathematics & informatics
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• v.22 no.1_2
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• pp.21-38
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• 2006

Programmed grammars, one of the most important and well investigated classes of grammars with context-free rules and a mechanism controlling the application of the rules, can be described by graphs. We investigate whether or not the restriction to special classes of graphs restricts the generative power of programmed grammars with erasing rules and without appearance checking, too. We obtain that Eulerian, Hamiltonian, planar and bipartite graphs and regular graphs of degree at least three are pr-universal in that sense that any language which can be generated by programmed grammars (with erasing rules and without appearance checking) can be obtained by programmed grammars where the underlying graph belongs to the given special class of graphs, whereas complete graphs, regular graphs of degree 2 and backbone graphs lead to proper subfamilies of the family of programmed languages.

• Journal of the Korean Mathematical Society
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• v.54 no.5
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• pp.1505-1519
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• 2017

Assume that M is an R-module where R is a commutative ring. A proper submodule N of M is called a weakly 2-absorbing primary submodule of M if $0{\neq}abm{\in}N$ for any $a,b{\in}R$ and $m{\in}M$, then $ab{\in}(N:M)$ or $am{\in}M-rad(N)$ or $bm{\in}M-rad(N)$. In this paper, we extended the concept of weakly 2-absorbing primary ideals of commutative rings to weakly 2-absorbing primary submodules of modules. Among many results, we show that if N is a weakly 2-absorbing primary submodule of M and it satisfies certain condition $0{\neq}I_1I_2K{\subseteq}N$ for some ideals $I_1$, $I_2$ of R and submodule K of M, then $I_1I_2{\subseteq}(N:M)$ or $I_1K{\subseteq}M-rad(N)$ or $I_2K{\subseteq}M-rad(N)$.

• Bulletin of the Korean Mathematical Society
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• v.50 no.2
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• pp.649-657
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• 2013

Let R be a commutative Noetherian ring, I an ideal of R and T be a non-zero I-cofinite R-module with dim(T) ${\leq}$ 1. In this paper, for any finitely generated R-module N with support in V(I), we show that the R-modules $Ext^i_R$(T,N) are finitely generated for all integers $i{\geq}0$. This immediately implies that if I has dimension one (i.e., dim R/I = 1), then $Ext^i_R$($H^j_I$(M), N) is finitely generated for all integers $i$, $j{\geq}0$, and all finitely generated R-modules M and N, with Supp(N) ${\subseteq}$ V(I).

• The Pure and Applied Mathematics
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• v.3 no.2
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• pp.155-162
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• 1996

We will show that let X and Y be M -finite Banach spaces with canonical M-decompositions $X{\cong}{{\prod}^{{\gamma}_{\infty}}_{i=1}}{X^{n_i}}_{i}\;and\;Y{\cong}{{\prod}^{{\bar{\gamma}}_{\infty}}_{j=1}}{\tilde{Y}^{m_j}}_{j}$, respectively and M and N nonzero locally compact Hausdorff spaces. Then I : $C_{0}$(M,X) ${\longrightarrow}\;C_{0}$(N,Y) is an isometrical isomorphism if and only if r = $\bar{r}$ and there are permutation and homeomorphisms and continuous maps such that I = ${I^{-1}}_{N.Y}\;{\circ}I_{w}^{-1}{\circ}({{\prod}^{\gamma}}_{i=1}I_{t_i,u_i}){\circ}I_{M,X}$.