Browse > Article

Effect of Bovine Colostral Whey Fraction containing Insulin-like Growth Factor on Cell Proliferation  

황경아 (성균관대학교 식품생명공학과)
양희진 (성균관대학교 식품생명공학과)
하월규 (파스퇴르유업㈜)
이수원 (성균관대학교 식품생명공학과)
Publication Information
Food Science of Animal Resources / v.24, no.2, 2004 , pp. 171-175 More about this Journal
Abstract
Insulin-like growth factor-I (IGF-I) rich fraction, which was obtained molecules ranged between 30 kDa and 1 kDa, was fractionated by ultrafiltration from bovine colostral whey with 30 kDa and 1 kDa membrane. IGF-I included in fractionated IGF-I rich fraction was confirmed by SDS-PAGE and western blotting and then the quantity of IGF-I was measured by ELISA. IGF-I concentration in IGF-I rich fraction was 10ng/mg protein. Effect of IGF-I rich fraction on in vitro proliferation of several cells was tested. IEC-6 cell proliferation rate was increased 60％. 53％, 30％, and 20％ at l0ng, 1ng, 0.1ng and IGF-I of IGF-I, respectively, compared to control group which was not supplemented by IGF-I rich fraction. IGF-I rich fraction stimulated in vitro proliferation of IEC-6 cell in a dose dependent manner by increasing cell number. Detroit 551 cell proliferation was enhanced 56％ and 26％ at 10ng and 1ng level of IGF-I, respectively, compared to control group. EL-4 cell and L6 cell proliferation was increased 53％ and 46％ at 10ng of IGF-I, respectively, compared to control group.
Keywords
IGF-I; colostral whey; ultrafiltration; cell proliferation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Einspanier, R. and Schams, D. (1991) Changes in con-centrations of insulin-like growth factor-1, insulin and growth hormone in bovine mammary gland secretion ante and post pactum. J. Dairy Res. 58(2), 171-178   DOI   ScienceOn
2 Marin, M. L., Murtha, J., Dong, W., and Pestka, J. J. (1996) Effects of mycotoxins on cytokine production and prolife-ration EL-4 thymoma cells. J. Toxicol. Environ. Health 48(4), 379-396   DOI   PUBMED
3 Simmen, F. A., Cera, K. R., and Mahan, D. C. (1990) Stimulation by colostrum or mature milk of gastrointestinal tissue development in newborn pigs. J. Anim. Sci. 68(11), 3596-3603   PUBMED
4 Qureshi, F. G., Tchorzewski, M. T., Dimcan, C. J., and Harmon, J. W. (1997) EGF and IGF-I synergistically stimulate proliferation of human esophageal epithelial cells. J. Surg. Res. 69(2), 354-358   DOI   ScienceOn
5 Sejrsen, K, Pedersen, L. O., Vestergaard, M., and Pump, S. (2001) Biological activity of bovine milk contribution of IGF-I and IGF binding proteins. Livestock Production Science. 70, 79-85   DOI
6 Hossenlopp, P., Seurin, D., Segovia-Quinson, B., Hardouin, S., and Binoux, M. (1986) Analysis of serum insulin-like growth factor binding protein using western blotting: use of the method for titration of the binding proteins and competitive binding site. Anal. Biochem. 154(1), 138-143   DOI   ScienceOn
7 Pakkanen, R. and Aalto, J. (1997) Growth factors and antimicrobial factors of bovine colostrum. Int. Dairy Journal. 7, 285-297   DOI   ScienceOn
8 Hossner, K. L. and Yemm, R. S. (2000) Improved recovery of insulin-like growth factors(IGFs) from bovine colostrum using alkaline diafiltration. Biotechnol. Appl. Biochem. 32 (3), 161-166   DOI   ScienceOn
9 Battelli, M.G., Abbondanza, A., Musiani, S., Buonamici, P. Trocchi, P. L., Tazzari, L., and Gramantieri, F. S. (1999) Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA). Clinica. Chimica. Acta. 281, 147   DOI   ScienceOn
10 Uramkpa, F. O., Ismond, M. A. H., and Akobundu, E. N. T. (2002) Colostrum and its benefits. Nutr. Res. 22, 755-767   DOI   ScienceOn
11 Monzavi, R. and Cohen, P. (2002) IGFs and IGFBPs : role in health and disease. Best Pract. Rese. Clin. Endocrinol. Metab. 16(3), 433-437   DOI   ScienceOn
12 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685   DOI   PUBMED   ScienceOn
13 Belford, D. A., Rogers, M. L., Regester, G. O., Francis, G. L., Smithers, G. W., Liepe, I. J., Priebe, I. K., and Ballard, F. J. (1995) Milk-derived growth factors as serum supplements for the growth of fibroblast and epithelial cells. In Vitro Cell. Dev. BioI. Ani. 31, 752-760   DOI   ScienceOn