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http://dx.doi.org/10.5483/BMBRep.2018.51.7.032

Investigation of functional roles of transcription termination factor-1 (TTF-I) in HIV-1 replication  

Park, Seong-Hyun (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Yu, Kyung-Lee (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Jung, Yu-Mi (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Lee, Seong-Deok (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Kim, Min-Jeong (Avixgen Inc.)
You, Ji-Chang (National Research Laboratory for Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
Publication Information
BMB Reports / v.51, no.7, 2018 , pp. 338-343 More about this Journal
Abstract
Transcription termination factor-1 (TTF-I) is an RNA polymerase 1-mediated transcription terminator and consisting of a C-terminal DNA-binding domain, central domain, and N-terminal regulatory domain. This protein binds to a so-called 'Sal box' composed of an 11-base pair motif. The interaction of TTF-I with the 'Sal box' is important for many cellular events, including efficient termination of RNA polymerase-1 activity involved in pre-rRNA synthesis and formation of a chromatin loop. To further understand the role of TTF-I in human immunodeficiency virus (HIV)-I virus production, we generated various TTF-I mutant forms. Through a series of studies of the over-expression of TTF-I and its derivatives along with co-transfection with either proviral DNA or HIV-I long terminal repeat (LTR)-driven reporter vectors, we determined that wild-type TTF-I downregulates HIV-I LTR activity and virus production, while the TTF-I Myb-like domain alone upregulated virus production, suggesting that wild-type TTF-I inhibits virus production and trans-activation of the LTR sequence; the Myb-like domain of TTF-I increased virus production and trans-activated LTR activity.
Keywords
c-Myb; Gene Regulation; HIV-1; HIV-1 Long Terminal Repeat; TTF-I; Virus Latency; Virus Replication;
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Times Cited By KSCI : 1  (Citation Analysis)
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