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Estimation of Genetic Parameters for The Growth Traits of Performance and Progeny Test in Hanwoo(Bos taurus Coreanae) (한우 당대검정우와 후대검정우의 성장형질에 관한 유전모수 추정)

  • Ki, K.S.;Choi, T.J.;Kim, S.D.;Choi, H.S.;Baik, D.H.
    • Journal of Animal Science and Technology
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    • v.49 no.6
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    • pp.699-710
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    • 2007
  • This study was conducted to estimate the genetic parameters and their relationships with weight traits of the steers and bulls in the Hanwoo population. The data used were weights and weight gain of performance and progeny test from 6,024 heads of Hanwoo. Data of performance test consisted of total 3,737 heads raised from August, 1989 to September, 2005. The number of the records of progeny test was total 2,287 heads from August, 1996 to June, 2004. The heritabilities and correlations for the body weights at the ages of 6 months, 12 months and 24 months and average daily gain were estimated by DFREML. Overall means and standard deviations of body weights at 6 and 12 months of age and average daily gain(ADG) from the data of performance test were 181.72±30.22kg, 351.48±40.24kg, 998.07±153.84g, respectively. Overall means and standard deviations of body weights at 6, 12, and 24 month of age and ADG from the data of progeny test were 169.18±32.82kg, 229.37±44.57kg, 570.45±64.36kg and 739.41± 172.14g, respectively. The heritability estimates of the body weight at 6, 12 month and ADG from the performance test were 0.54±0.06, 0.60±0.06 and 0.23±0.04, respectively. The heritability estimates of the body weight at 6, 12, 24 month and ADG from the progeny test were 0.80±0.08, 0.50±0.07, 0.46±0.07 and 0.07±0.03, respectively.

Production of In-Vitro Fertilized Embryos and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle (한우 및 젖소에서 체외 수정란 생산과 신선 및 동결 수정란 이식 결과)

  • Kim Y. J.;Kim H. C.;Seo S. H.;Jeong K. N.;Kim Y. S.;Lee H. R.;Shin D. S.;Jo S. W.;Kim S. H.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.79-87
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    • 2005
  • The ovaries from Hanwoo and Holstein were collected from labattoir and transferred to laboratory. Oocytes were aspirated and incubated in $CO_2$ incubator for 24 hours for maturation. Oocytes were coincubated with the sperms for 5 hours. Cleaved oocytes were selected 48 hours after coincubation and half of the medium was changed newly every 48 hour until blastocyst formation. Cleavage rate and blastocyst rate were investigated according to different breeds and different status of cumulus cells surrounding the oocytes. Blastocysts were either transferred to the recipients or frozen until use. The result of embryo transfer with fresh or frozen embryos was investigated. The rate of male offspring following embryo transfer was also investigated. The rate of cleavage was $66.4\%$ for Hanwoo and $62.4\%$ for Holstein oocytes. The rate of cleavage according to status of oocyte was shown highest in the oocytes completely surrounded with cumulus cells and lowest in denuded oocytes for both Hanwoo and Holstein oocytes. The rate of blastocyst from cleaved oocytes was $40.6\%$ for Hanwoo and $36.9\%$ for Holstein. The rate of pregnancy/delivery following embryo transfer with fresh IVF embryos was $57.2\%$ for Hanwoo and $53.3\%$ for Holstein. The rate of pregnancy/delivery following embryo transfer with frozen IVF embryos was $40.9\%$ for Hanwoo and $36.4\%$ for Holstein. The rate of male calf produced by embryo transfer was $63.6\%$ for Hanwoo and $50.0\%$ for Holstein.

Clinical Study of Acute and Chronic Pain by the Application of Magnetic Resonance Analyser $I_{TM}$ (자기공명분석기를 이용한 통증관리)

  • Park, Wook;Jin, Hee-Cheol;Cho, Myun-Hyun;Yoon, Suk-Jun;Lee, Jin-Seung;Lee, Jeong-Seok;Choi, Surk-Hwan;Kim, Sung-Yell
    • The Korean Journal of Pain
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    • v.6 no.2
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    • pp.192-198
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    • 1993
  • In 1984, a magnetic resonance spectrometer(magnetic resonance analyser, MRA $I_{TM}$) was developed by Sigrid Lipsett and Ronald J. Weinstock in the USA, Biomedical applications of the spectrometer have been examined by Dr. Hoang Van Duc(pathologist, USC), and Nakamura, et al(Japan). From their theoretical views, the biophysical functions of this machine are to analyse and synthesize a healthy tissue and organ resonance pattern, and to detect and correct an abnormal tissue and organ resonance pattern. All of the above functions are based on Quantum physics. The healthy tissue and organ resonance patterns are predetermined as standard magnetic resonance patterns by digitizing values based on peak resonance emissions(response levels or high pitched echo-sounds amplified via human body). In clinical practice, a counter or neutralizing resonance pattern calculated by the spectrometer can correct a phase-shifted resonance pattern(response levels or low pitched echo-sounds) of a diseased tissue and organ. By administering the counter resonance pattern into the site of pain and trigger point, it is possible to readjust the phase-shifted resonance pattern and then to alleviate pain through regulation of the neurotransmitter function of the nervous system. For assessing clinical effectiveness of pain relief with MRA $I_{TM}$ this study was designed to estimate pain intensity by the patient's subjective verbal rating scale(VRS such as graded to no pain, mild, moderate and severe) before application of it, to evaluate an amount of pain relief as applied the spectrometer by the patients subjective pain relief scale(visual analogue scale, VAS, 0~100%), and then to observe a continuation of pain relief following its application for managing acute and chronic pain in the 102 patients during an 8 months period beginning March, 1993. An application time of the spectrometer ranged from 15 to 30 minutes daily in each patient at or near the site of pain and trigger point when the patient wanted to be treated. The subjects consisted of 54 males and 48 females, with the age distribution between 23~40 years in 29 cases, 41~60 years in 48 cases and 61~76 years in 25 cases respectively(Table 1). The kinds of diagnosis and the main site of pain, the duration of pain before the application, and the frequency of it's application were recorded on the Table 2, 3 and 4. A distinction between acute and chronic pain was defined according to both of the pain intervals lasting within and over 3 months. The results of application of the spectrometer were noted as follows; In 51 cases of acute pain before the application, the pain intensities were rated mild in 10 cases, moderate in 15 cases and severe in 26 cases. The amounts of pain relief were noted as between 30~50% in 9 cases, 51~70% in 13 cases and 71~95% in 29 cases. The continuation of pain relief appeared between 6~24 hours in two cases, 2~5 days in 10 cases, 6~14 days in 4 cases, 15 days in one case, and completely relived of pain in 34 cases(Table 5~7). In 51 cases of chronic pain before the application, the pain intensities were rated mild in 12 cases, moderate in l8 cases and severe in 21 cases. The amounts of pain relief were noted as between 0~50% in 10 cases, 51~70% in 27 cases and 71~90% in 14 cases. The continuation of pain relief appeared to have no effect in two cases. The level of effective duration was between 6~12 hours in two cases, 2~5 days in 11 cases, 6~14 days in 14 cases, 15~60 days in 9 cases and in 13 cases the patient was completely relieved of pain(Table 5~7). There were no complications in the patients except a mild reddening and tingling sensation of skin while applying the spectrometer. Total amounts of pain relief in all of the subjects were accounted as poor and fair in 19(18.6%) cases, good in 40(39.2%) cases and excellent in 43(42.2%) cases. The clinical effectiveness of MRA $I_{TM}$ showed variable distributions from no improvements to complete relief of pain by the patient's assessment. In conclusion, we suggest that MRA $I_{TM}$ may be successful in immediate and continued pain relief but still requires several treatments for continued relief and may be gradually effective in pain relief while being applied repeatedly.

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Assessment of Microbiological Quality for Raw Materials and Cooked Foods in Elementary School Food Establishment (초등학교에 공급되는 급식용 식재료 및 조리식품의 미생물학적 품질평가)

  • Shin, Weon-Sun;Hong, Wan-Soo;Lee, Kyung-Eun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.3
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    • pp.379-389
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    • 2008
  • This study was conducted to assess the microbiological quality of raw and cooked foods served in the elementary school food service. Raw and cooked food samples were collected from 11 selected elementary schools in both June to July and September to October of 2005. Petrifilm plates were used to determine (in duplicate) total aerobic colony counts (PAC), Enterobacteriaceae (PE), coliform counts (PCC), and E. coli counts (PEC). Heavy contamination of Enterobacteriaceae (from 0.08 to 7.40 log CFU/g) and total coliform (0.50 to 6.52 log CFU/g) were observed in raw materials and cooked foods. Escherichia coli (E. coli) were detected in the sample of currant tomato (3.70 log CFU/g), sesame leaf (3.59 log CFU/g), dropwort (0.20 log CFU/g), crown daisy (3.15 log CFU/g), parsley (3.00 log CFU/g), peeled green onion (1.74 log CFU/g), frozen pork (0.65 log CFU/g), frozen beef (0.20 or 1.50 log CFU/g), chicken (1.78 log CFU/g), and young radish leaf seasoned with soybean paste (1.24 log CFU/g). Multiplex PCR system was used to determine the food-borne pathogens: Salmonella spp., Bacillus cereus (B. cereus), E. coli O157:H7, Staphylococcus aureus, Listeria monocytogenes (L. monocytogenes), Vibrio parahaemolyticus, Campylobacter jejuni (C. jejuni), Shigella spp., B. cereus was detected in 19 samples of raw materials and 8 samples of cooked foods. With regard to quantitative analysis, B. cereus counts exceeded 5.46, 3.48 and 1.79 log CFU/g in sesame leaf, peeled green onion and seasoned mungbean jelly, respectively. E. coli O157:H7 was detected on 2 samples of frozen beefs, and its biochemical characteristics of one beef sample was confirmed with API 20E kit (93.7%). L. monocytogenes was detected in fried rice paper dumpling, but the presumptive colonies were not detected onto the conventional plate. C. jejuni was detected in peeled & washed onion.

Weight Control and Cardiovascular Risk in Middle-Aged Women (중년여성의 체중관리 실태와 성인병 위험도)

  • Kim, Jeong-Ah;Chaung, Seung-Kyo
    • The Korean Journal of Rehabilitation Nursing
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    • v.7 no.1
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    • pp.33-47
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    • 2004
  • The purpose of this study was to investigate weight control practices and cardiovascular risk in middle-aged women. 304 middle-aged women were selected as subjects from thirties to fifthies living at J city in Chung-Buk Do. Data were collected using a questionnaire, anthropometric measurements, BP & total cholesterol level in serum from April 1, to June 30, 2003. The results of this study were as follows: The middle-aged woman's age is average $43.95{\pm}7.09yr$ and mean BMI(body mass index) was $23.54{\pm}3.09\;kg/m^2$. Underweight, normal weight, overweight, obese women were 3.0%, 39.5%, 27.9% and 29.6%respectively. Subjects perceived own as 'Slim' 5.9%, 'ordinary' 45.7%, 'Fat' 48.4%. Their weight perception coincide their own actual body weight but as many as 41.8% of overweight and 9.0% of obese perceived themselves as being 'ordinary'. Middle age women with past weight control experience were 55.6%, and only 35.5% was doing weight control at present. Of these subjects, 71.4% reported wanting to lose weight and the primary reason of weight control was to improve their appearance(39.53%). The most frequently reported weight control behavior was 'exercise' followed 'dieting', but 39.6% reported using 'diet food', 12.4% 'behavior modification', 12.4% 'fasting', and 'diet-drug'(3.6%) or 'smoking'(3.6%). Effective weight control methods were thought regular exercise(97.1%) & dieting (79.3%). And behavior modification(71.4%) or diet camp(60%) were effective, too. An average waist circumference was $79.80{\pm}9.47cm$, waist/hip ratio was $86.63{\pm}6.78$, waist/height ratio was $50.43{\pm}6.10$. In the index of abdominal obesity, 79.7 % of middle-aged women was waist/height ratio over 0.46, 65.3% was waist/hip ratio over 0.85, 28.4% was waist circumference over 85cm. There were significant differences in the degree of abdominal obesity according to age and BMI. In the index of cardiovascualr risk, 10.9% of middle aged women was systolic hypertension over 140mmHg, 18.7% was diastolic hypertension over 90mmHg and 10.6% was hypercholesterolemia over 200mg/dl. There was significant difference in systolic hypertension ratio according to age. There was significant difference in diastolic hypertension ratio according to age and obesity. There were significant differences in hypercholesterolemia accorting to obesity. The abdominal obesity indices and the levels of T-cholesterol in the serum, systolic and diastolic BP increased significantly according to age. T-cholesterol in serum was predicted 2.6% by waist/height ratio. And systolic BP was predicted 15.2% by waist/height, add BMI to 16.8%. Subject's diastolic BP was predicted 12.1% by BMI. Therefore waist/height ratio and BMI were significant factors for the predictors of cardiovascular risk. There was significant correlation between index of obesity and cardiovascular risk. T-cholesterol in serum had correlation with waist/eight ratio(r=0.174) and waist circumference(r=0.48). Systolic BP had correlation with waist/height ratio(r=0.387), and BMI(=0.371). diastolic BP correlation had correlation with BMI(r=0.343) and waist/height ratio(r=0.327). In conclusion, The prevalence of obesity was 29.6% in 304 cases, and increased as age after menopause increased. Middle-aged women's weight perception and actual BMI coincide but some of them did not. Trial to reduce weight was attempted. But most of them did not actually. Undesirable weight control method such as using drugs, fasting, smoking was used by some women. It is important to educate about health weight control methods and raise their awareness of exact body figures. High frequency of abdominal obesity in middle-aged women had correlation with hypertension and hypercholesterolemia. Abdominal index such as waist/height ratio, waist circumference, waist/hip ratio was used. Women's hypertension, hypercholesterolemia significantly related to body mass index and abdominal obesity.

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Antimicrobial and Antioxidative Activities of the Extracts from Walnut (Juglans regia L.) Green Husk (호두과피 추출물의 항산화 및 항균활성)

  • Han, Kook-Il;Kim, Mi ran;Jo, Bu Kyung;Kim, Min Ji;Kang, Min Joo;Park, Ki-hyoun;Koo, Ye eun;Kim, Byeongseong;Jung, Eui-Gil;Han, Man-Deuk
    • Journal of Life Science
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    • v.25 no.4
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    • pp.433-440
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    • 2015
  • Several studies suggest that regular consumption of walnuts may have beneficial effects against oxidative stress-mediated disease such as cancer. The present study reports the total phenolic and flavonoid contents, together with the antioxidant and antibacterial activities of several solvent extracts (methanol, n-hexane, ethyl acetate, n-butanol, and water) obtained from walnut (Juglans regia L.) green husk. MIC (minimal inhibitory concentration) values of the walnut extracts for 8 human pathogenic bacteria strain were determined using agar dilution method. Antioxidant activity of extracts were assessed using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) assays, EC50 of DPPH and ABTS scavenging activities, and determination of total phenolic and flavonoid content and its correlation with DPPH and ABTS scavenging capacities. Among the six extracts, ethyl acetate extract (EtOAc Ex) showed the highest antimicrobial activity at 3.2 mg/ml of MICs against Staphylococcus aureus SG511. Total flavonoids and polyphenol contents of EtOAc Ex were 42.48 mg of quercetin equivalents (QE)/g and 223.25 mg of gallic acid equivalents (GAE)/g respectively. The highest antioxidative potential was shown by the sample extracted with EtOAc Ex (EC50=13.43 μg/ml for DPPH and EC50=41.83 μg/ml for ABTS radical scavenging activity assay). These results showed that J. regia green husk extracts can be used as an easily accessible source of natural antibacterial agents and natural antioxidants.

Determination of Energy Requirements for Maintenance in Hanwoo Steers (거세한우의 유지에너지 요구량 결정)

  • Kim, K.H.;Oh, Y.G.;Kim, W.;Lee, S.C.;Shin, K.J.;Jeon, B.T.
    • Journal of Animal Science and Technology
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    • v.46 no.2
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    • pp.193-200
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    • 2004
  • This experiment was carried out to determine energy requirements for maintenance of Hanwoo steers. Nine Hanwoo steers weighing 376.6$\pm$12.5kg were used in this experiment and fed rice straw(44%) and concentrate (56%) at three different energy levels; 0.8 times maintenance(0.8M), 1.2 times(1.2M) and 1.6 times(1.6M), respectively. Dry matter intake was 48.5, 65.9 and 86.5g/$BW^{0.75}$ for 0.8M, 1.2M and 1.6M, respectively. Increase in energy intake with the increased DM intake did not affect digestibilities of dry matter, organic matter, crude protein, crude fiber, crude fat and nitrogen-free extract. Gross energy intake averaged 190.8, 255.8 and 340.9kcal/BW0.75 for 0.8M, 1.2M and 1.6M, respectively. Energy loss was 41% feces and 0.6${\sim}$1.5% urine of gross energy intake. Further, energy loss from methane produced during rumen fermentation was 5${\sim}$9%, while body heat loss averaged 40${\sim}$60%. Intercept of the regression equation between ME intake and retained energy indicated that energy requirement for maintenance was 124.3kcal $ME/BW^{0.75}$.

Retrieval of the Variation of Optical Characteristics of Asian Dust Plume according to their Vertical Distributions using Multi-wavelength Raman LIDAR System (다파장 라만 라이다 관측을 통한 황사의 이동 고도 분포에 따른 광학적 특성 변화 규명)

  • Shin, Sung-Kyun;Park, Young-San;Choi, Byoung-Choel;Lee, Kwonho;Shin, Dongho;Kim, Young J.;Noh, Youngmin
    • Korean Journal of Remote Sensing
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    • v.30 no.5
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    • pp.597-605
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    • 2014
  • The continuous observations for atmospheric aerosols were conducted during 3 years (2009 to 2011) by using Gwangju Institute of Science and Technology (GIST) multi-wavelength Raman lidar at Gwangju, Korea ($35.10^{\circ}N$, $126.53^{\circ}E$). The aerosol depolarization ratios calculated from lidar data were used to identify the Asian dust layer. The optical properties of Asian dust layer were different according to its vertical distribution. In order to investigate the difference between the optical properties of each individual dust layers, the transport pathway and the transport altitude of Asian dust were analyzed by Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT) model. We consider that the variation of optical properties were influenced not only their transport pathway but also their transport height when it passed over anthropogenic pollution source regions in China. The lower particle depolarization ratio values of $0.12{\pm}0.01$, higher lidar ratio of $67{\pm}9sr$ and $68{\pm}9sr$ at 355 nm and 532 nm, respectively, and higher ${\AA}ngstr\ddot{o}m$ exponent of $1.05{\pm}0.57$ which are considered as the optical properties of pollution were found. In contrast with this, the higher particle depolarization ratio values of $0.21{\pm}0.09$, lower lidar ratio of $48{\pm}5sr$ and $46{\pm}4sr$ at 355 nm and 532 nm, respectively, and lower ${\AA}ngstr\ddot{o}m$ exponent of $0.57{\pm}0.24$ which are considered as the optical properties of dust were found. We found that the degree of mixing of anthropogenic pollutant aerosols in mixed Asian dust govern the variation of optical properties of Asian dust and it depends on their altitude when it passed over the polluted regions over China.

Growth Environment and Vegetation Structure of Cephalotaxus koreana Nakai in South Korea Natural Habitats (국내 개비자나무 자생지 생육환경 및 식생구조)

  • Kim, Young Ki;Kim, Joon Seon;Lee, Kap Yeon;Kim, Moon Sup
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.384-395
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    • 2018
  • This study was carried out to investigate the environment factors including community structure and soil characteristics in the wild habitats of Cephalotaxus koreana, and offers the basic information for habitats conservation and restoration. Most of the wild habitats were located at altitudes between 148~835 m with inclinations ranged as $12{\sim}32^{\circ}$. The average soil pH was 4.7~5.9, soil organic matter was 5.72~15.99%, cation exchange capacity was $14.1{\sim}19.9cmolc/kg^{-1}$ and exchangeable $K^+$, $Ca^{2+}$, $Mg^{2+}$ was 0.25~0.48 cmolc/kg, 0.79~6.68 cmolc/kg, 0.31~1.73 cmolc/kg, respectively. The dominant species of tree layer were found to be dominated by Quercus dentata in Jekbo-san (C1), Acer pictum in Bogae-san (C2), Acer pseudosieboldianum in Geumwon-san (C3), Q. serrata in Jiri-san (C4), Zelkova serrata in Baegun-san (C5), and Q. acutissima in Duryun-san (C6). The Species diversity (H') was 0.854~1.234, evenness (J') was 0.654~0.993, and dominance (D) was found to be 0.067~0.346. Correlation coefficients analysis based on environmental factors, community structure and value of species diversity shows that growth of Cephalotaxus koreana is correlated with species diversity and evenness. This result show that Cephalotaxus koreana habitats located in mature stands.

Study on the Influence of Ca and Mg Saturation Ratios of Soil on the Uptake of Ca, Mg, and K by Rice Plant (답토양(畓土壤)의 Ca, Mg 포화비(飽和比)가 수도(水稻)의 Ca, Mg, K의 흡수(吸收)에 미치는 영향(影響))

  • Jeong, Y.G.;Hong, J.W.;Ha, H.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.115-121
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    • 1987
  • A pot experiment was conducted to investigate the influence of Ca and Mg saturation ratios of soil on the uptake of Ca, Mg and K by rice plant. A silty loam soil was treated with $CaCl_2$ and $MgCl_2$ to obtain different degrees of Ca and Mg saturation. The studied ranges of Ca and Mg saturation ratios were 81:19, 70:30, 52:48, 55:45, and 31:69 in terms of the ratio of exchangeable Ca and Mg. Two levels of K application (90kg/ha, and 180kg/10a as $K_2O$) were also included in this study. The significant observation were summarized as follows. 1. When the Ca saturation of soil was dominant over Mg, the soil solution contained more Ca than Mg and vice versa. These led to the higher uptake of Ca by rice plant in Ca dominant soils and higher uptake of Mg in Mg dominant soils. 2. When the Ca and Mg saturation ratio was about equal, more Mg was released to soil solution leading to higher concentration of Mg in rice plant compare to that of Ca. 3. A trend was observed that the concentration of K in soil solution was lower in Mg dominant soils than in Ca dominant soils. This also resulted in the depressed uptake of K by rice plant under Mg dominant system when compare to Ca dominant system. 4. The increase application of K led to the increase in relative concentration of K to (Ca+Mg+K), and to the depression of divalent uptake by rice plant. However, it was observed that the degree of depression in uptake divalent by K application was more sensitive in case of Mg than that of Ca. 5. When viewed from grain yield of rice, it is pointed out that the optimum range of Ca to Mg ratio in soil may fall in the vicinity of 7:3. 6. Although K uptake by rice plant was influenced by the term of $AK^+/{\sqrt{A(Ca^{{+}{+}}+Mg^{{+}{+}})$ in soil solution, $AK^+$ itself was affected by the ratios of Ca:Mg in soil, as it were $AK^+$ value was decreased in Mg dominant soil than in Ca.

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