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Production of In-Vitro Fertilized Embryos and Result of Transfer with Fresh or Frozen Embryos for Hanwoo and Holstein Cattle  

Kim Y. J. (College of Veterinary Medicine, Chonbuk National University)
Kim H. C. (College of Veterinary Medicine, Chonbuk National University)
Seo S. H. (Jeonju-Zoo)
Jeong K. N. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do)
Kim Y. S. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do)
Lee H. R. (College of Veterinary Medicine, Chonbuk National University)
Shin D. S. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do)
Jo S. W. (Livestock Breeding Station, Livestock Development & Research Institute of Chonrabuk-do)
Kim S. H. (College of Veterinary Medicine, Chonbuk National University)
Publication Information
Journal of Embryo Transfer / v.20, no.2, 2005 , pp. 79-87 More about this Journal
Abstract
The ovaries from Hanwoo and Holstein were collected from labattoir and transferred to laboratory. Oocytes were aspirated and incubated in $CO_2$ incubator for 24 hours for maturation. Oocytes were coincubated with the sperms for 5 hours. Cleaved oocytes were selected 48 hours after coincubation and half of the medium was changed newly every 48 hour until blastocyst formation. Cleavage rate and blastocyst rate were investigated according to different breeds and different status of cumulus cells surrounding the oocytes. Blastocysts were either transferred to the recipients or frozen until use. The result of embryo transfer with fresh or frozen embryos was investigated. The rate of male offspring following embryo transfer was also investigated. The rate of cleavage was $66.4\%$ for Hanwoo and $62.4\%$ for Holstein oocytes. The rate of cleavage according to status of oocyte was shown highest in the oocytes completely surrounded with cumulus cells and lowest in denuded oocytes for both Hanwoo and Holstein oocytes. The rate of blastocyst from cleaved oocytes was $40.6\%$ for Hanwoo and $36.9\%$ for Holstein. The rate of pregnancy/delivery following embryo transfer with fresh IVF embryos was $57.2\%$ for Hanwoo and $53.3\%$ for Holstein. The rate of pregnancy/delivery following embryo transfer with frozen IVF embryos was $40.9\%$ for Hanwoo and $36.4\%$ for Holstein. The rate of male calf produced by embryo transfer was $63.6\%$ for Hanwoo and $50.0\%$ for Holstein.
Keywords
in-vitro fertilization; embryo transfer; fresh embryo; frozen embryo;
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Times Cited By KSCI : 3  (Citation Analysis)
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