• Journal of Animal Environmental Science
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• v.17 no.2
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• pp.81-92
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• 2011

When we bury the infected poultry into the ground, we have many problems such as the difficulty of making sufficient area for burying, environmental contamination by the leachate, unpleasant ordor. Also, in case of burning the carcass of the infected poultry, there are some problems such as high cost, dust, unpleasant odor, etc. It could cause environmental contamination which many peoples and environmental organization complains about. In this study, we develop a treating system which treats the infected poultry carcass in a environmental method preventing the environment contamination. This system is composed of many processes. The euthanasia system uses rigid vinyl to trap and to do a euthanasia the infected poultry with lethal gas, carbon dioxide. And then, with the tractor attached grappler infected poultry carcass could be put into the carcass treating system. The euthanasia system uses rigid vinyl to trap the infected birds and to confine lethal gas, carbon dioxide. Infected poultry carcass are moved to carcass disposal system by collecting device which is attached at tractor. The carcass treatment system (capacity of disposal : 6.3 $m^3$) is installed on a truck and do one pass work, which is input, crush, stir, sterilize, and discharge treated carcass. 1,000 chickens was killed within 9.7min by $CO_2$ (300L/min) in the tent (10 $m^3$). The collecting device could carry 142 chickens at a time, and the movable carcass treatment system could sterilize 2 tons carcass per hour (at one time). This treatment systems was eco-friendly because it reduced the volume of carcass by 31.9% with no wastewater generation.

• Korean Journal of Poultry Science
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• v.26 no.2
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• pp.131-144
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• 1999

Coccidiosis, an intestinal infection caused by intracellular protozoan parasites belonging to several different species of Eimeria seriously impairs the growth and feed utilization of livestock and poultry. Due to complex life cycle of organism and intricate host immune responses to Elmeria, coccidia vaccine development has been difficult. Understanding of basic imunobiology of pertinent host-parasite interactions is necessary for the development of novel control strategy. Although chickens infected with Eimeria spp. produce parasite-specific antibodies in both the circulation and mucosal secretions, antibody mediated responses play a minor role in protection gainst coccidiosis. Rather, increasing evidence show that cell-mediated immunity plays a major role in resistance to coccidiosis. T-lymphocytes appear to respond to coccidiosis both through cytokine production and a direct cytotoxic attack on infected cells. The exact mechanisms by which T-cells eliminate the parasites, however, remain to be investigated. Since it is crucial to understand the intestinal immune system in order to develop an immunological control strategy against any intestinal immune system in order to develop an immunological control strategy against any intestinal diseases, this presentation will summarize our current understanding of the avian intestinal immune system and mucosal immune responses to Eimeria, to provide a conceptual overview of the complex molecular and cellular events involved in intestinal immune responses to enteric pathogens.

• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.267-272
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• 2023

This study was conducted to investigate the effect of integrated pest management (IPM) with 3point 5score microscopic monitoring method for laying farms infected with poultry red mites. A total of 8 laying farms infected with poultry red mites were divided into two treatments: 1) integrated pest management with 3point 5score microscopic monitoring method and 2) normal pest management with 3point 5score microscopic monitoring method. The results revealed that there is no significant difference between the groups with respect to the score of poultry red mites not only regardless of disinfection for the laying farms but also from week 4 to week 20 (P>0.05). However, the farm with IPM had a lower (P<0.05) score than those with the normal pest management method on week 24. Based on the result of the present study, controlling the presence of poultry red mites using IPM, along with consistent monitoring and supplementation using the 3point 5score microscopic monitoring method, is deemed effective in reducing the ultimately targeted infection rate of poultry red mites.

• Journal of Animal Environmental Science
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• v.16 no.2
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• pp.153-160
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• 2010

Recently, the treatment of dead poultry has become more important issue because, the infected poultry, which was buried under the ground, causes environmental contaminations such as steep water and reek occurrence, etc. Therefore, in this study, we investigated the type of treatment and the composting methods influencing to the characteristics on decomposition and fermentative disinfection of dead poultry with poultry manure and sawdust. The results of the port tests showed that amputated poultry treated by the cut-sterilization were not only more decomposed, with less smell compared to the non-treated poultry carcass. When we treated thermophilic microorganism such as bacillus in this amputated poultry, the temperature of treated poultry increased much fester, the fermentation temperature didn't rise and not maintained constantly for long time due to the small size of the fermentation port. On the other hand, we did fermentation test by the layered disposal method with more poultry. In this experiment, the temperature of fermented poultry rose to $54^{\circ}C$ in a day and maintained around $55^{\circ}C$ during four weeks period. With less odor outside the experiment room. further. Also, we inoculated AI virus, ND virus in the excrement for studying the effect of fermentative disinfection. The result of the test revealed that AI virus was destructed within 60 minutes and ND virus was destructed within 30 minutes at the temperature of $56^{\circ}C$. Therefore, the investigations revealed scope of composting method for steam sterilized infected poultry in the originated area mixed with poultry manure, sawdust by thermophilic microorganism could increase the effectiveness of fermentative disinfection and decrease the environmental contamination.

• Journal of the Korean Association of Geographic Information Studies
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• v.19 no.3
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• pp.114-126
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• 2016

Domestic risk factors that are thought to be correlated with highly pathogenic avian influenza (HPAI) outbreak are migratory birds and moving objects such as poultry farm vehicles. In particular, the commercial vehicles that routinely circulate the local and/or remote poultry farms produce are thought to be major HPAI risk factors in South Korea. In this study, the driving histories of the vehicles belonging to poultry farms and/or commercial companies registered in the Korea Animal Integrated System (KAHIS) were analyzed using statistical and social networking tools in a Geographic Information System (GIS) in order to understand the pattern of the HPAI (H5N8) outbreak that occurred in 2014 in South Korea. Based on the 2014 HPAI outbreak patterns, HPAI-infected poultry farms were categorized according to geological features. The HPAI-infected poultry farms were categorized as 'regional-accumulation', 'regional-distribution', 'metropolitan-accumulation', 'metropolitan-distribution' and 'national-distribution' in endemic or non-endemic regions. We were able to categorize most HPAI-infected poultry farms into the five proposed categories, but further studies are required to categorize all such farms. Based on this categorization system, we propose efficient but economical prevention boundaries in South Korea. We strongly believe that our research could hugely impact government decisions to estimate the prevention area.

• Korean Journal of Poultry Science
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• v.6 no.1
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• pp.38-46
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• 1979

This study was directed to the Peizootics of Newcastle disease in Korea. Since 1960 New-castle disease epizootics have been observed at an interval of one or two years. The frequency of Newcastle disease outbreaks was related to the amount of the vaccine used, being the more sufficient vaccine produced the fewer outbreaks. Regional distribution of Newcastle disease outbreaks was most frequent in the capital area, being the center of poultry raising and Poultry product marketing, and infrequent in areas distant from the capital. Spreading of Newcastle disease appears to be mainly due to the movement of infected chickens. More than 60 percent of the total outbreaks of Newcastle disease diagnosed at the Veterinary Research Institute in Anyang were observed in young chickens aged less than 60 days. Improper vaccination, interference by maternally derived antibody with the immune response following vaccination, ana immaturity of immune systems of young chickens might have contributed to the results. This view was further supported by the findings that 23 percent of the total flocks experienced Newcastle disease were not vaccinated at all and, in vaccinated flocks, virtually all of the flocks were vaccinated by the drinking water method, which has been known less effective compared to individual application or spraying methods.

• Parasites, Hosts and Diseases
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• v.59 no.4
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• pp.403-408
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• 2021

Avian coccidiosis has a major economic impact on the poultry industry, it is caused by 7 species of Eimeria, and has been primarily controlled using chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. We assessed anticoccidial effects of berberine-based diets in broiler chickens following oral infection with 5 Eimeria species (E. acervulina, E. maxima, E. tenella, E. mitis, and E. praecox). When 0.2% berberine, a concentration that does not affect weight gain, was added to the diet, the 4 groups infected with E. acervulina, E. tenella, E. mitis, or E. praecox showed significant reductions in fecal oocyst shedding (P<0.05) compared to their respective infected and untreated controls. In chickens treated 0.5% berberine instead of 0.2% and infected with E. maxima, fecal oocyst production was significantly reduced, but body weight deceased, indicating that berberine treatment was not useful for E. maxima infection. Taken together, these results illustrate the applicability of berberine for prophylactic use to control most Eimeria infections except E. maxima. Further studies on the mechanisms underlying the differences in anticoccidial susceptibility to berberine, particularly E. maxima, are remained.

• Animal Bioscience
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• v.35 no.7
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• pp.964-974
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• 2022

Objective: The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry and economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for studies on HPAIV resistance. Therefore, in this study, we investigated gene expression related to the mitogen-activated protein kinase (MAPK) signaling pathway by comparing non-infected, HPAI-infected resistant, and susceptible Ri chicken lines. Methods: Resistant (Mx/A; BF2/B21) and susceptible Ri chickens (Mx/G; BF2/B13) were selected by genotyping the Mx and BF2 genes. Then, the tracheal tissues of non-infected and HPAIV H5N1 infected chickens were collected for RNA sequencing. Results: A gene set overlapping test between the analyzed differentially expressed genes (DEGs) and functionally categorized genes was performed, including biological processes of the gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. A total of 1,794 DEGs were observed between control and H5N1-infected resistant Ri chickens, 432 DEGs between control and infected susceptible Ri chickens, and 1,202 DEGs between infected susceptible and infected resistant Ri chickens. The expression levels of MAPK signaling pathway-related genes (including MyD88, NF-κB, AP-1, c-fos, Jun, JunD, MAX, c-Myc), cytokines (IL-1β, IL-6, IL-8), type I interferons (IFN-α, IFN-β), and IFN-stimulated genes (Mx1, CCL19, OASL, and PRK) were higher in H5N1-infected than in non-infected resistant Ri chickens. MyD88, Jun, JunD, MAX, cytokines, chemokines, IFNs, and IFN-stimulated expressed genes were higher in resistant-infected than in susceptible-infected Ri chickens. Conclusion: Resistant Ri chickens showed higher antiviral activity compared to susceptible Ri chickens, and H5N1-infected resistant Ri chickens had immune responses and antiviral activity (cytokines, chemokines, interferons, and IFN-stimulated genes), which may have been induced through the MAPK signaling pathway in response to H5N1 infection.

• Journal of Veterinary Science
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• v.23 no.1
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• pp.2.1-2.18
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• 2022

Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.

• 한국환경농학회:학술대회논문집
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• 2009.07a
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• pp.220-227
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• 2009

For last about 10 years, the Republic of Korea experienced 3 times of outbreaks of highly pathogenic avian influenza (HPAI) from 10 December 2003 to 30 April 2004 (a total number of 19 outbreaks), 22 November 2006 to 6 March 2007 (a total number of 7 outbreaks), and 1 April 2008 to 12 May 2008 (a total number of 33 outbreaks). Among the totally 59 outbreaks, the infected premises included 35 chicken farms, 17 duck farms, 1 quail farm, and 6 farms rearing mixed species. Control measures were applied according to the HPAI standard operation procedure including depopulation of all infected and suspected flocks, movement restrictions, and disinfection of the infected farms within a 500-meter radius. Including movement restrictions, stringent control measures were additionally applied to two designated zones: the protection zone was an area within a 3-kilometer radius of the outbreak farm, and the surveillance zone was an area between a 3- to 10-kilometer radius of the outbreak farm. Farms with dangerous contacts and/or all of poultry within the protection zone was subjected to preemptive culling. Epidemiological investigations were also carried out including trace-back and trace-forward investigations to identify possible sources of spread and dangerous contact farms. Investigation teams conducted on-site examination of farm premises and facilities, interview with farm owner and staff, and review of records. Genetic and pathogenic characteristics of the virus isolates, and the results of the various surveillance activities were also analyzed. HPAI surveillance conducted in Korea includes passive surveillance of investigating notified cases, and active surveillance of testing high risk groups and areas. HPAI is a notifiable disease in Korea and all suspect cases must be reported to the veterinary authorities. Cases reported for other poultry diseases that require differential diagnosis are also tested for HPAI. Active surveillance includes annual testing of breeder duck farms, broiler duck farms and wild bird surveillance, which is concentrated during the autumn and winter. Surveillance activities conducted prior to the outbreaks have shown no evidence of HPAI infection in Korea.