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http://dx.doi.org/10.4142/jvs.21139

Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in the lungs of piglets  

Zhang, Jing (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Wang, Jing (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Zhang, Xiong (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Zhao, Chunping (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Zhou, Sixuan (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Du, Chunlin (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Tan, Ya (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Zhang, Yu (College of Animal Science, Guizhou University)
Shi, Kaizhi (Key Laboratory of Livestock and Poultry Major Epidemic Disease Monitoring and Prevention, Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences)
Publication Information
Journal of Veterinary Science / v.23, no.1, 2022 , pp. 2.1-2.18 More about this Journal
Abstract
Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.
Keywords
Porcine reproductive and respiratory syndrome virus; Haemophilus parasuis; co-infection; immune response; RNA sequencing;
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