• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.195-198
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• 1994

A bubble counter was designed and fabricated for the measurement of gas production rate on the basis of number of bubbles produced from yeast fermentor. The sensor was consisted of bubble forming device and electronic signal processing circuitry. The bubble forming device was built with bubble collector and liquid cell to form uniform size of bubble. Bubbles were counted by pulses formed by photo-interrupter circuitry having 8-bit binary latch counter. The gas production rate curves on the basis of bubble counted showed a good agreement to that of growth curves obtained by the optical measurement method. The sensor was succesfully applied to monitoring of the nutrient utilization test with glucose and galactose media.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.12
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• pp.1626-1632
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• 2018

In this paper, we describe the fabrication and characterization of a hydrogen peroxide ($H_2O_2$) sensor based on palladium and copper (PdCu) electroplated laser induced graphene (LIG) electrodes. $CO_2$ laser was used to form LIG electrodes on a PI film. This fabrication method allows simple control of the LIG electrode size and shape. The PdCu was electrochemically deposited on the LIG electrodes to improve the electrocatalytic reaction with $H_2O_2$. The electrochemical performance of this sensor was evaluated in terms of selectivity, sensitivity, and linearity. The physical characterization of this sensor was conducted using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), which confirmed that PdCu was formed on the laser induced graphene electrode. In order to increase the sensor sensitivity, the Pd:Cu ratio of the electroplated PdCu was varied to five different values and the condition of highest amperometric current at an identical of $H_2O_2$ concentration was chosen among them. The resulting amperometric current was highest when the ratio of Pd:Cu was 7:3 and this Pd;Cu ratio was employed in the sensor fabrication. The fabricated PdCu/LIG electrode based $H_2O_2$ sensor exhibited a sensitivity of $139.4{\mu}A/mM{\cdot}cm^2$, a broad linear range between 0 mM and 16 mM of $H_2O_2$ concentrations at applied potential of -0.15 V, and high reproducibility (RSD = 2.6%). The selectivity of the fabricated sensors was also evaluated by applying ascorbic acid, glucose, and lactose separately onto the sensor in order to see if the sensor ourput is affected by one of them and the sensor output was not affected. In conclusion, the proposed PdCu/LIG electrode based $H_2O_2$ sensor seems to be suitable $H_2O_2$ sensor in various applications.

• Analytical Science and Technology
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• v.7 no.3
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• pp.285-291
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• 1994

A microbial BOD sensor for the continuous estimation of BOD was been developed by immobilizing Trichosporon cutaneum, which was immobilized between a dialysis and a gas-permeable membrane, on an oxygen electrode. The optimum pH and temperature for BOD measurement using this sensor were pH 7.0 and $32{\sim}33^{\circ}C$, respectively. The best result was obtained at 2~3ml/min flow rate in 0.1M phosphate buffer solution. A linear relationship was observed between ${\Delta}DO$ and the concentration of standard GGA solution below 60mg/l(90ppm $BOD_5$). The reproducibility was found to be within 3% for the standard solution containing glucose 30mg/l and glutamic acid 30mg/l. The output DO value of this sensor was almost constant for 30 dalys. The response time and the recovery time were about 5 and 10 min, respectively. This sensor was employed for the BOD measurement of waste pollutants and was compared with $BOD_5$ method.

• Journal of Sensor Science and Technology
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• v.23 no.6
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• pp.388-391
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• 2014

The most promising application of the biofuel cells is implantable devices, so the biofuel cells should have an appropriate shape for the vascular vessel. We demonstrated the biofuel cell roll for using in tubes. MWNTs were aggregated by vacuum filtration on a nitrocellulose membrane filter, which was biocompatible and flexible. The MWNT aggregated nitrocellulose membrane used the electrodes of the biofuel cells because it was conductive as well as nanostuructured. Then, the membrane was rolled into the roll shape. The maximum power density of the biofuel cell roll was $7.9{\mu}W/cm^2$ at 153mV and 50 mM glucose. Also, the power density is expected to increase in its practical application if there is flow in the tube, which makes the transportation of fuel easy. The biofuel cell roll contacts with the wall of the tube, so flow in the tube does not disturb. Also, the biofuel cell roll has multi-layers offering more electroactive area.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.29 no.4
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• pp.247-255
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• 2018

In this work, glucose solution and sodium chloride solution were distinguished noninvasively using a microwave complementary split-ring resonator (CSRR). Based on the electrical properties of the two solutions measured using a open-ended coaxial probe, a CSRR was designed and fabricated for operation at a specific frequency that facilitates differentiating the two solutions. Furthermore, a polydimethylsiloxane mold was fabricated to concentrate the solution at a region where the electric field of the resonator was strongest, and a laminating film was used to prevent contact between the solution and resonator. Experiments were performed by dropping $50{\mu}L$ of the solution in steps of 100 mg/dL up to a maximum human blood glucose level of 400 mg/dL. Our experiments confirmed that the transmission coefficients ($S_{21}$) of glucose solution and sodium chloride solution exhibit variations of -0.06 dB and 0.14 dB, respectively, per 100 mg/dL concentration change at the resonance frequency. Thus, the opposite trends in the variation of $S_{21}$ with change in the concentration of the two solutions can be used to distinguish between them.

• Polymer(Korea)
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• v.33 no.2
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• pp.111-117
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• 2009

This study aims to verify for humans the suitability of the enzyme-fixed hydrogel used for the patch sensor of the blood sugar testing system without blood sampling, which utilizes reverse iontophoresis. Using acrylate monomers, hydrogel was synthesized to which a certain unit of enzyme is fixed. In order to analyze the material property of the synthesized hydrogel, a structural analysis was performed using FT-IR spectroscopy, while the DSC was used to verify the thermal stability. In addition, with the UV-Vis spectrophotometer, it was verified that the degree of active enzyme is at least 50% greater than the standard product. The SEM was used to verify secure fixation of the enzyme onto the surface. As a result, it was observed that the enzyme is successfully fixed to the surface. Since the hydrogel makes direct contact with a patient's skin, it is essential to evaluate the toxicity when making direct contact with the skin. For that purpose, various sets of tests were undertaken according to the ISO 10993-cytotoxicity, intracutaneous reactivity, skin irritation test and maximization sensitization. Consequently, it was successfully verified that the enzyme-fixed hydrogel have bioavailability.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.2
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• pp.345-352
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• 2017

In this paper, a unified potentiostat which can measure the current of both $O_2$-based and $H_2O_2$-based blood glucose sensors with low supply voltage of 1.0V has been designed and verified by simulations and measurements. Potentiostat is composed of low-voltage operational transconductance amplifier, cascode current mirrors and mode-selection circuits. It can measure currents of blood glucose chemical reactions occurred by $O_2$ or $H_2O_2$. The body of PMOS input differentional stage of the operational transconductance amplifier is forward-biased to reduce the threshold voltage for low supply voltage operation. Also, cascode current mirror is used to reduce current measurement error generated by channel length modulation effects. The proposed low-voltage potentiostat is designed and simulated using Cadence SPECTRE and fabricated in Magnachip 0.18um CMOS technology with chip size of $110{\mu}m{\times}60{\mu}m$. The measurement results show that consumption current is maximum $46{\mu}A$ at supply voltage of 1.0V. Using the persian potassium($K_3Fe(CN)_6$) equivalent to glucose, the operation of the fabricated potentiostat was confirmed.

• Macromolecular Research
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• v.14 no.2
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• pp.251-254
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• 2006

• Journal of Mechanical Science and Technology
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• v.20 no.5
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• pp.668-674
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• 2006

In order to develop a cell based robot, we present a micro-mechanical force measurement system for the biological muscle actuators, which utilize glucose as a power source. The proposed measurement system is composed of a micro-manipulator, a force transducer with a glass probe, a signal processor, an inverted microscope and video recording system. Using this measurement system, the contractile force and frequency of the cardiac myocytes were measured in real time and the magnitudes of the contractile force of each cardiac myocyte under different conditions were compared. From the quantitative experimental results, we could estimate that the force of cardiac myocytes is about $20\sim40{\mu}N$, and show that there are differences between the control cells and the micro-patterned cells.

• KSBB Journal
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• v.13 no.3
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• pp.251-258
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• 1998

A structured kinetic model is proposed to describe cell growth and secondary metabolite, flavone glycosides, synthesis in batch suspension culture of Scutellaria baicalensis G. The model has been developed by representing the physiological state of cell described as the activity and viability which can be estimated based on the culture fluorescence. In the model, three type of cells are considered; active-viable, nonactive-viable and dead cells. Viable cell weight could be determined based on the relative fluorescence intensity. The flavone glycosides could be produced by both active-viable and non-active viable cells with a different production rate. And the model includes the cell expansion due to glucose concentration and death phase which accounts for the release of intracellular secondary metabolite into medium. Dependent variables include substrate concentration(glucose), cell mass(dry cell weight and fresh cell weight), product concentration(flavone glycosides), activity and viability. Satisfactory agreement between the model and experimental data is obtained from shake flask culture of Scutellaria baicalensis G. The proposed model can predict the cell growth and flavone glycosides synthesis as well as intermediate materials.