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Contractile Force Measurements of Cardiac Myocytes Using a Micro-manipulation System  

Park Suk-Ho (Microsystem Research Center, Korea Institute of Science and Technology)
Ryu Seok-Kyu (Microsystem Research Center, Korea Institute of Science and Technology)
Ryu Seok-Chang (Microsystem Research Center, Korea Institute of Science and Technology)
Kim Deok-Ho (Microsystem Research Center, Korea Institute of Science and Technology)
Kim Byung-Kyu (School of Aerospace & Mechanical Engineering, Hankuk Aviation University)
Publication Information
Journal of Mechanical Science and Technology / v.20, no.5, 2006 , pp. 668-674 More about this Journal
Abstract
In order to develop a cell based robot, we present a micro-mechanical force measurement system for the biological muscle actuators, which utilize glucose as a power source. The proposed measurement system is composed of a micro-manipulator, a force transducer with a glass probe, a signal processor, an inverted microscope and video recording system. Using this measurement system, the contractile force and frequency of the cardiac myocytes were measured in real time and the magnitudes of the contractile force of each cardiac myocyte under different conditions were compared. From the quantitative experimental results, we could estimate that the force of cardiac myocytes is about $20\sim40{\mu}N$, and show that there are differences between the control cells and the micro-patterned cells.
Keywords
Cardiac Myocytes; Cell Force Measurement; Micro-Manipulation; Piezo Resistive; Sensor;
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