• Journal of Plant Biotechnology
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• 제42권1호
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• pp.19-24
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• 2015

식물은 여러 환경스트레스에 적응하기 위해 스트레스 내성 유전자의 발현 혹은 proline, trehalose, glycine betaine (GB) 등과 같이 삼투압을 조절하는 compatible solute를 생성하면서 진화해 왔다. GB는 고염, 저온 등 환경스트레스 조건에서 식물의 엽록체에서 축적되는 물질 중 하나이다. 토양 박테리아 Arthrobacter globiformis에서 분리한 choline oxidase (codA) 유전자는 choline을 GB로 전환하는 기능을 한다. 본 연구에서는 산화스트레스 유도성 SWPA2 프로모터의 발현조절 하에 codA 유전자를 엽록체에 과발현시킨 형질전환 고구마 식물체(SC식물체)를 제작하여 다양한 환경스트레스 조건에서의 특성을 분석하였다. SC 식물체는 methyl viologen (MV)에 의한 산화스트레스와 건조 처리 조건에서 내성 증가를 보였다. $5{\mu}M$ MV 처리시 형질전환 식물체는 GB의 함량이 증가하였고 낮은 수준의 이온 전도도를 보였다. 건조 스트레스 조건에서 형질전환 식물체는 codA 유전자의 발현이 증가하였으며, 대조구 보다 높은 상대수분함량을 유지하였다. 따라서 본 연구결과의 SC식물체는 고염, 건조토양 등 조건 불리지역에 재배하면 바이오매스를 증가시킬 수 있을 것으로 예상된다.

• Korean Journal of Acupuncture
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• 제32권3호
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• pp.108-115
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• 2015

Objectives : Acupuncture is frequently used as an alternative therapy for Parkinson's disease(PD) in Korea. Using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson's disease mouse model, the present study investigated a possible role of acupuncture stimulation at GB34 in suppressing dopaminergic neuronal death and regulating the phosphorylation of protein kinase B(Akt) in substantia nigra(SN) and striatum(ST). Methods : Eight-week-old male C57BL/6 mice were administered intraperitoneally with 30 mg/kg of MPTP at 24-h intervals for 5 days. Acupuncture stimulation at GB34 or SI3 was performed once a day for 12 days consecutively from the first MPTP injection. After the last acupuncture stimulation, pole test was performed to assess the effect of the acupuncture stimulations. Dopaminergic neuronal survival in the SN and the ST, dopamine transporter( DAT) and caspase-3 expression in the ST were evaluated by immunohistochemistry. The phosphorylations of Akt in the SN and the ST were measured by Western blotting. Results : MPTP administration caused behavioral impairment and dopaminergic neuronal death in the SN and the ST. It also decreased DAT expression and increased caspase-3 expression in the ST. Acupuncture stimulation at GB34 alleviated these MPTP-induced impairments. Moreover, MPTP suppressed Akt phosphorylation in the SN and the ST, whereas acupuncture stimulation at GB34 alleviated the phosphorylation in the SN. Conclusions : These results indicate that acupuncture stimulation at GB34 can inhibit MPTP-induced dopaminergic neuronal death and alleviate the Akt phosphorylation in the SN, suggesting a possible role for acupuncture in the treatment of PD.

• 한방재활의학과학회지
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• 제25권1호
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• pp.17-26
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• 2015

Objectives This study was carried out to investigate the experimental effects of Lonicerae Caulis and Bee-venom Acupuncture in Yanglingquan (陽陵泉, GB34) that have clinical efficacy in the Rheumatoid Arthritis. Methods Materials of present study are Lonicerae Caulis Extracts (LCE), Bee-Venom Acupuncture (BVP), Sprague-dawley rats (250 g or so, ♂), and various kinds of needing experimental studies. We measured several experimental items of the rats with the arthritis induced by Freund's complete adjuvant (0.2 ml/kg), such as body weight, rate of paw edema, analgesic effect by hot plate method, WBC, TNF-${\alpha}$ cytokine and IL-10 cytokine. Rats were divided into four groups; Normal group that was treated with normal saline 1.0 ml (o.p) and $15{\mu}l/kg$ (GB34) to normal rats, Control group that was treated with normal saline 1.0 ml (o.p) and $15{\mu}l/kg$ (GB34) to pathologic model rats induced by Freund's complete adjuvant 0.2 ml/kg, Experimental group A that was treated with LCE 1.0 ml (o.p) and normal saline $15{\mu}l/kg$ (GB34) to pathologic model rats induced by Freund's complete adjuvant 0.2 ml/kg and Experimental group B that was treated with LCE 1.0 ml (o.p) and BVP $15{\mu}l/kg$ (GB34) to pathologic model rats induced by Freund's complete adjuvant 0.2 ml/kg, and the experiment took over after 28 days. The results were analysed using SPSS for windows 12.0. Results Experimental group A showed the increase in body weight, paw licking times and IL-10 cytokine compared to Control group. Also it was decreased in rate of paw edema, WBC, and TNF-${\alpha}$ cytokine compared with Control group. Experimental group B showed the increase in body weight, paw licking times, and IL-10 cytokine compared to Control group, and showed the decrease in rate of paw edema, WBC and TNF-${\alpha}$ cytokine compared to Control group. Especially TNF-${\alpha}$ cytokine and rate of paw edema were accepted statistical significance compared with Control group. Conclusions It is suggested that Lonicerae Caulis (o.p) and Bee-venom Acupuncture (GB34) can be used in the treatment of rheumatoid arthritis.

• 한국유기농업학회지
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• 제10권3호
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• pp.79-86
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• 2002

GB$_{10}$, Chitofarm 및 왕겨숯과 왕겨숯 液 등 天然物質을 가을 무 栽培圃場에 種子處理, 土壤處理 및 葉面撒布를 實施하여 收量과 成分分析으로 遂行한 實驗 成績은 다음과 같다. 1. 무 生育特性 중 株重, 根重, 根長 및 根莖에 있어서 Chitofarm 등 天然物質處理區가 無處理區에 대하여 大部分 有意差가 있었으나 天然物質處理區 사이에는 有意性이 없었다. 2. 무 收量에 있어서는 GB$_{10}$ 1%葉面撒布區가 1.7kg으로 check區 보다 75% 增收되었다. 3. 무 作物體 成分分析結果 T-N, $P_2O_5,\;K_2O$,CaO, MgO, Fe, Mn 및 Zn 등의 含量에서 天然物質 無處理區보다 多量이었으므로 品質向上에 效果的이었다.

• ETRI Journal
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• 제27권3호
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• pp.312-318
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• 2005

Mid-span spectral inversion (MSSI) has to utilize high optical pump power, for its operation principle is based on a nonlinear optical wavelength conversion. In this paper, a low pump-power operation of MSSI-based chromatic dispersion compensation (CDC) has been achieved successfully, for the first time to our knowledge, by introducing a noise pre-reduction scheme in cascaded wavelength conversions with periodically poled $LiNbO_3$ waveguides at a relatively low operation temperature. As preliminary studies, phase-matching properties and operation-temperature dependence of the wavelength converter (WC) were characterized. The WC pumped at 1549 nm exhibited a wide conversion bandwidth of 59 nm covering the entire C-band and a conversion efficiency of -23.6 dB at 11 dBm pump power. CDC experiments were implemented with 2.5 and 10 Gb/s transmission systems over 100 km single-mode fiber. Although it is well-known that the signal distortion due to chromatic dispersion is not critical at a 2.5 Gb/s transmission, the clear recovery of eye patterns was identified. At 10 Gb/s transmission experiments, eye patterns were retrieved distinctly from seriously distorted ones, and notable improvements in bit-error rates were acquired at a low pump power of 14 dBm.

• Journal of Acupuncture Research
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• 제21권3호
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• pp.179-191
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• 2004

It is well known that acupuncture is effective for treatment of gastric disease in human. For years, we have been utilizing a electro-acupuncture(EA) in attempt to manage gastric symptoms. However, the data on clinical effectiveness is conflicting and the action of acupoints is unclear. The purpose of this study was to investigate the effects of manual and electro acupuncture(EA) at acupoints of ST 36 and GB 34 on the serum gastrin level by radioimmunoassay in rats. All of acupuncture treatment was applied to the acupoints for 30 min during 3 days. Manual acupuncture with both acupoints resulted in increased gastrin serum level when compare to control group treated with only anesthesia. Either high (100 Hz) and low (2 Hz) frequency EA at ST 36 decreased gastrin serum level compared with control group, but EA at GB 34 conversely increased. In EA with device was designed stimulating only acupoint(New EA), either low frequency on ST 36 and high frequency on GB 34 unusually increased gastrin serum level compared to control group. The results of this experiment suggest that many factors, such as the selection of acupoints, the manipulation and the specification of acupuncture device, are capable of affecting acupuncture effects.

• Journal of Acupuncture Research
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• 제27권4호
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• pp.137-146
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• 2010

Objectives & Methods : The purpose of this study is to observe the effects of Cnidii Rhizoma Herbal solution at $GB_{34}$(Yangleungchean) on hyperlipidemia in rats. The author performed several experimental items to analyze the levels of various components and enzymes in serum, urine and liver, as well as the histological change of liver and aorta. Results : 1. In the Cndii Rhizoma Herbal-acupuncture group, The levels of total cholesterol were significantly decreased, and the ratio of HDL to Total cholesterol, Phospholipid/total cholesterol were significantly increased as compared with those of the control group. 2. In the Cndii Rhizoma Herbal-acupuncture group, HMG-CoA reductase activity was significantly decreased than those of the control group NP(needle prick) group and saline group. 3. In the Cndii Rhizoma Herbal-acupuncture group, phathohistological change of liver was suppressed as compared with the control group. Conclusions : From the above results, it is suggested that Cndii Rhizoma Herbal-acupuncture at $GB_{34}$ has a therapeutic effect on hyperlipidemia.

• IEIE Transactions on Smart Processing and Computing
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• 제5권3호
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• pp.207-214
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• 2016

Optical communication systems are rapidly spread following increases in data traffic. In this work, a 32-Gb/s inductorless output buffer circuit with adjustable pre-emphasis is proposed. The proposed circuit consists of an output buffer circuit and an emphasis circuit. The emphasis circuit emphasizes the high frequency components and adds the characteristics of the output buffer circuit. We proposed a design method using a small-signal equivalent-circuit model and designed the compensation characteristics with a 65-nm CMOS process in detail using HSPICE simulation. We also realized adjustable emphasis characteristics by controlling the voltage. To confirm the advantages of the proposed circuit and the design method, we fabricated an output buffer IC with adjustable pre-emphasis. We measured the jitter and eye height with a 32-Gb/s input using the IC. Measurement results of double-emphasis showed that the jitter was 14% lower, and the eye height was 59% larger than single-emphasis, indicating that our proposed configuration can be applied to the design of an output buffer circuit for higher operation speed.

• 대한전자공학회논문지
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• 제17권4호
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• pp.45-52
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• 1980

집적회로화된 연산증폭기의 GB종의 유한성으로 인한 위상오차가 능동여피기의 사용가능주파수를 5 KHz 내외로 한정하고 있는 문제점을 해소하기 위하여 새로운 형태의 적분기를 제안하였다. 이 적분기의 위상오차와 선택도가 정의 적호를 가지고 있어서 Thomas의 biquad회로망내의 Miller 적분기와 대치하면 원래의 부의 위상오차를 보상하여 전체회로망특성을 개선한다. Thoma의 biquad 도로를 본보상법에 의해서 개조한 대역통과여파기의 주파수특성을 측정한바 중심주파수가 20KHz 그리고 Q가 100에 이르기까지 만족할만한 응답을 보여 특성의 개선을 확인할 수 있었다.

• 한국광학회지
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• 제14권4호
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• pp.454-459
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• 2003

단일 모드 광원이 집적된 Mach-Zehnder간섭계형 파장 변환기를 제작하여 세계 최초로 10 Gb/s에서 파장 변환 특성을 확인하였다. 제작된 파장 변환기는 수동 도파로 영역에서의 전파 손실을 줄이기 위해 undoped InP층이 수동 도파로 위에 형성된 새로운 BRS 구조를 사용하였다. 단일 모드 광원으로 손실 결합형 분포 궤환형 반도체 레이저(loss-coupled distributed feedback laser; LC-DFB LD)를 사용하여, 파장 변환기에 있는 반도체 광증폭기의 주입전류가 200 mA까지 측모드 억제율이 30 dB 이상의 값을 나타내었다. 제작된 LC-DFB LD 집적 파장 변환기는 10 Gb/s의 동적 파장 변환 특성 측정 결과, 7 dB 정도의 소광비를 갖는 eye 패턴을 얻을 수 있었으며, power penalty는 $10^{-9}$ bit error rate에서 2.8 dB의 값을 나타내었다.