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http://dx.doi.org/10.18325/jkmr.2015.25.1.017

Experimental Study of Lonicerae Caulis and Bee-venom Acupuncture on the Rheumatic Pathologic Model Induced by Adjuvant in Rats  

Bae, Myung-Hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-kwang University)
Yeam, Seung-Ryong (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-kwang University)
Kim, Jin-Hee (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-kwang University)
Kwon, Young-Dal (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Won-kwang University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.25, no.1, 2015 , pp. 17-26 More about this Journal
Abstract
Objectives This study was carried out to investigate the experimental effects of Lonicerae Caulis and Bee-venom Acupuncture in Yanglingquan (陽陵泉, GB34) that have clinical efficacy in the Rheumatoid Arthritis. Methods Materials of present study are Lonicerae Caulis Extracts (LCE), Bee-Venom Acupuncture (BVP), Sprague-dawley rats (250 g or so, ♂), and various kinds of needing experimental studies. We measured several experimental items of the rats with the arthritis induced by Freund's complete adjuvant (0.2 ml/kg), such as body weight, rate of paw edema, analgesic effect by hot plate method, WBC, TNF-${\alpha}$ cytokine and IL-10 cytokine. Rats were divided into four groups; Normal group that was treated with normal saline 1.0 ml (o.p) and $15{\mu}l/kg$ (GB34) to normal rats, Control group that was treated with normal saline 1.0 ml (o.p) and $15{\mu}l/kg$ (GB34) to pathologic model rats induced by Freund's complete adjuvant 0.2 ml/kg, Experimental group A that was treated with LCE 1.0 ml (o.p) and normal saline $15{\mu}l/kg$ (GB34) to pathologic model rats induced by Freund's complete adjuvant 0.2 ml/kg and Experimental group B that was treated with LCE 1.0 ml (o.p) and BVP $15{\mu}l/kg$ (GB34) to pathologic model rats induced by Freund's complete adjuvant 0.2 ml/kg, and the experiment took over after 28 days. The results were analysed using SPSS for windows 12.0. Results Experimental group A showed the increase in body weight, paw licking times and IL-10 cytokine compared to Control group. Also it was decreased in rate of paw edema, WBC, and TNF-${\alpha}$ cytokine compared with Control group. Experimental group B showed the increase in body weight, paw licking times, and IL-10 cytokine compared to Control group, and showed the decrease in rate of paw edema, WBC and TNF-${\alpha}$ cytokine compared to Control group. Especially TNF-${\alpha}$ cytokine and rate of paw edema were accepted statistical significance compared with Control group. Conclusions It is suggested that Lonicerae Caulis (o.p) and Bee-venom Acupuncture (GB34) can be used in the treatment of rheumatoid arthritis.
Keywords
Lonicerae Caulis; Bee-venom Acupuncture; Yanglingquan; GB34; Rheumatoid Arthritis; Freund's complete adjuvant;
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