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http://dx.doi.org/10.5010/JPB.2015.42.1.19

Enhanced drought and oxidative stress tolerance in transgenic sweetpotato expressing a codA gene  

Park, Sung-Chul (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Myoung Duck (Institute of Genetic Engineering, Hankyong National University (HNU))
Kim, Sun Ha (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Yun-Hee (Department of Biology Education, College of Education, Gyeongsang National University)
Jeong, Jae Cheol (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Haeng-Soon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.42, no.1, 2015 , pp. 19-24 More about this Journal
Abstract
Glycine betaine (GB) is one of the compatible solutes that accumulate in the chloroplasts of certain halotolerant plants under salt or cold stress. The codA gene for choline oxidase, the enzyme that converts choline into GB, has been cloned from a soil bacterium Arthrobacter globiformis. We generated transgenic sweetpotato plants [Ipomoea batatas (L.) Lam] expressing codA gene in chloroplasts under the control of the SWPA2 promoter (referred to as SC plants) and evaluated SC plants under oxidative and drought stresses. SC plants showed enhanced tolerance to methyl viologen (MV)-mediated oxidative stress and drought stress due to induced expression of codA. At $5{\mu}M$ of MV treatment, all SC plants showed enhanced tolerance to MV-mediated oxidative stress through maintaining low ion leakage and increased GB levels compared to wild type plants. When plants were subjected to drought conditions, SC plants showed enhanced tolerance to drought stress through maintaining high relative water contents and increased codA expression compared to wild type plants. These results suggest that the SC plants generated in this study will be useful for enhanced biomass production on global marginal lands.
Keywords
Sweetpotato; Glycine betaine; codA gene; Oxidative stress; Drought stress;
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Times Cited By KSCI : 2  (Citation Analysis)
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