• Title/Summary/Keyword: G-Rb₁/G-Rg₁

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Ginsenoside Contents and Antioxidative Activities from Red Ginseng Treated with High Hydrostatic Pressure (초고압 처리한 홍삼의 ginsenoside 함량 및 항산화 활성)

  • Shin, Chang-Sik;Lee, Do-Hyun;Kim, Sung-Han;Shin, Min-Ho;Jeong, Chang-Ho;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.133-140
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    • 2010
  • This study was carried out to investigate the changes of ginsenoside contents and antioxidative activities from red ginseng after treated with high hydrostatic pressure (RGHHP). Crude saponin content in traditional red ginseng (TRG) and RGHHP were 21.93 and 27.29 mg/g, respectively. The contents of total phenolics, crude saponin and ginsenoside increased after treated with high hydrostatic pressure. TRG and RGHHP showed an increase 25.60% the highest content of Rb1 (14.10 mg/g and 17.71 mg/g). Also, Rg3 content compared with TRG and RGHHP increased 10.46%. The radical scavenging activity of hot water extract from red ginseng against the DPPH and ABTS radicals increased with the increasing amount of extract and RGHHP higher than TRG. The reducing power and ferric reducing antioxidant power (FRAP) assays of the red ginseng were increased in a dose dependent manner. The FRAP of TRG and RGHHP were 0.30 and 0.36 absorbance, respectively at a concentration of 10 mg/mL. The present results suggest that RGHHP would have the protective potential from oxidative stress induced by free radicals.

Enhancement and Conversion of Ginsenoside Contents in Cultured Wild Ginseng Adventitious Root (산삼 부정배양근의 진세노사이드 함량 증진과 성분 변환)

  • Kim, Chul Joong;Choi, Jae Hoo;Oh, Yeong Seon;Seong, Eun Soo;Lim, Jung Dae;Yu, Chang Yeon;Lee, Jae Geun
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.6
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    • pp.445-454
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    • 2020
  • Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.

Effects of Compositions of Saponin Fraction from Korean Red Ginseng in the Relaxation of Rabbit and Rat Corpus Cavernosum (토끼와 흰쥐 음경해면체 이완작용에 미치는 홍삼사포닌 분획별 효과)

  • Choi Young Deuk;Park Jin Ah;Choi Hyung Ki;Nam Ki Yeul
    • Journal of Ginseng Research
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    • v.23 no.1 s.53
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    • pp.13-20
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    • 1999
  • We previously reported that Korean red ginseng (KRG) has a relaxation effect on the smooth muscles of corpus cavernosum via nitric oxide (NO) pathway and calcium and potassium channels. However, it is suggested that the active ingredients of KRG might be different depending on the sources of preparation, and there might be differences in actions for different compositions. We first investigated the composition of KRG saponins according to the extractions of the various sources of KRG, then with these extractions the relaxation effects were evaluated in vitro and hemodynamical in vivo using New Zealand white rabbit and rat corpus cavernosum. The total compositions of ginsenoside $(G-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;G-Re,\;-Rf,\;-Rg_1)$ in fractionated KRG saponin designated as TS-1, TS-2, TS-3 were $41\%,\;40\%,\;and\;62\%,$ respectively, and the ratios of PD saponin and PT saponin (PD/PT) were 1,55, 1.72, 2.25, and 2.61, the values of which were statistically significant. In vitro studies using the rabbit corpus cavernosal muscle strips, the KRG saponin relaxed cavernosal strips in a dose-dependent manner, and same results were observed in in vivo studies, that KRG saponin increased the intracavernosal pressure in the rat. There was difference in the efficacy according to fractionation techniques. The differences in the total contents of ginsenosides did not affect relaxation, rather PT saponin content was statistically related to the degree of cavernosal relaxation, and this action presumed to be mediated by NO pathway and calcium and potassium channels. In conclusion, KRG exerts relaxation which is a key step in erection via combination of effects on NO system or calcium and potassium channels. The efficacy of this action is different to the sources of ginseng, which is affected by the different composition of ginsenosides $(G-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;G-Re,\;-Rf,\;-Rg_1).$ Thus the further studies on the active ingredients such as minor ginsenosides and non-saponin components of red ginseng with maximum potency should be sought.

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Validation on the Analytical Method of Ginsenosides in Red Ginseng

  • Cho B. G.;Nho K. B.;Shon H. J.;Choi K. J.;Lee S. K.;Kim S. C;Ko S. R.;Xie P. S.;Yan Y. Z.;Yang J. W.
    • Proceedings of the Ginseng society Conference
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    • 2002.10a
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    • pp.491-501
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    • 2002
  • A cross-examination between KT&G Central Research Institute and Guangzhou Institute for Drug Control was carried out in order to select optimum conditions for extraction, separation and determination of ginsenosides in red ginseng and to propose a better method for the quantitative analysis of ginsenosides. The optimum extraction conditions of ginsenosides from red ginseng were as follows: the extraction solvent, $70\%$ methanol; the extraction temperature, $100^{\circ}C;$ the extraction time, 1 hour for once; and the repetition of extraction, twice. The optimum separation conditions of ginsenosides on the SepPak $C_{18}$ cartridge were as follows: the loaded amount, 0.4 g of methanol extract; the washing solvents, distilled water of 25 ml at first and then $30\%$ methanol of 25 ml; the elution solvent, $90\%$ methanol of 5 ml. The optimum HPLC conditions for the determination of ginsenosides were as follows: column, Lichrosorb $NH_2(25{\times}0.4cm,$ 5${\mu}m$, Merck Co.); mobile phase, a mixture of acetonitrile/water/isopropanol (80/5/15) and acetonitrile/water/isopropanol (80/20/15) with gradient system; and the detector, ELSD. On the basis of the optimum conditions a method for the quantitative analysis of ginsenosides were proposed and another cross-examination was carried out for the validation of the selected analytical method conditions. The coefficient of variances (CVs) on the contents of ginsenoside-$Rg_{1}$, -Re and $-Rb_1$ were lower than $3\%$ and the recovery rates of ginsenosides were $89.4\~95.7\%,$ which suggests that the above extraction and separation conditions may be reproducible and reasonable. For the selected HPLC/ELSD conditions, the CVs on the detector responses of ginsenoside-Rg, -Re and $-Rb_1$) were also lower than $3\%$, the regression coefficients for the calibration curves of ginsenosides were higher than 0.99 and two adjacent ginsenoside peaks were well separated, which suggests that the above HPLC/ELSD conditions may be good enough for the determination of ginsenosides.

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Application of Near Spectroscopy for Nondestructive Evaluation of Ginsenoside Content

  • Lin, Guo-Lin;Cho, Rae-Kwang;Hong, Jin-Hwan
    • Near Infrared Analysis
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    • v.1 no.2
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    • pp.45-48
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    • 2000
  • The effective component Ginsenoside in Ginseng has been widely used to cure some hypochondriasis and be as supplementary medicines. There are many chemical analysis methods to measure the contents of Ginsenoside in Ginseng; however, all these methods have some shortcomings such as long time, environmental pollution and damaging the samples. In this paper, it is possible to use near infrared spectroscopy to measure the content of Ginsenoside in Ginseng without destruction. As the results, Rg1, Rb1, Re and T-Saponin of Ginsenoside can be measured with the accuracy of R(0.81) SEP (1.704 mg/g), R(0.74) SEP (1.211 mg/g), R (0.78) SEP (1.049 mg/g) and R(0.84), SEP(4.537 mg/g).

Effects of Ginseng Saponin on the Cytokine Gene Expression in Human Immune System (인삼 사포닌이 인간면역계 사이토카인 유전자의 발현에 미치는 영향)

  • 박종욱;한인숙
    • Journal of Ginseng Research
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    • v.20 no.1
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    • pp.15-22
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    • 1996
  • In order to investigate the Immunomodulatory effects of ginseng, we have studied the effects of ginseng saponin on the proliferation and cytosine gene expression of human pheripheral blood mononuclear cell (PBMC). In the PBMC proliferation assay, total saponin exhibited proliferation inhibition on the PBMC or phytohemagglutinin(PHA)-stimulated PBMC in a dose-dependent fashion. Immunomodulatory effects of ginseng were further investigated using the cytokine gene expression as the indicators. In the reverse transcription-polymerase chain reaction (RT-PCR) test, interleukin (IL)-1, IL-2, IL-3, IL-4, IL-6, IL-13, granulocyte macrophage-colony stimulating factor, tumor necrosis factor (TNF), migration inhibitory factor and transforming growth factor genes were expressed in the PHA-stimulated PBMC 48 hrs after cell culture. Among expressed cytokines, total saponin could increase the expression of IL-1 and TNF of PBMC without stimulation of PHA. All of ginsenosides, $Rb_1$, $Rb_2$, $Rg_1$, Rc, Re, incresed TNF gene expression. Especially, Rb2 (20 g/ml) showed most prominent effect on TNF gene expression and it also slightly increased IL-1 gene expression of PBMC.

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Antioxidant Properties of Cultured Wild Ginseng Roots Extracts (산삼배양근 추출물의 항산화 특성)

  • Kim, Jae-Won;Lee, Shin-Ho;No, Hong-Kyoon;Hong, Joo-Heon;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.17 no.6
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    • pp.861-866
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    • 2010
  • We obtained hot-water extracts (HWE) and 70% (v/v) ethanol extracts (EE) from cultured wild ginseng roots (CWGR) and determined the saponin and total polyphenol contents, and antioxidant activities. The yields of freeze-dried powder from the HWE and EE were 27.86% and 18.33% (both w/w), respectively. The total polyphenol content of the EE (22.63 mg/g) was higher than that of the HWE (17.90 mg/g). Ginsenoside-Rb1 and -Rg1 contents of hot-air-dried CWGR were 17.90 mg/g and 22.63 mg/g, respectively. The electron-donating ability of HWE and EE were 2.82-60.58% and 3.88?70.88%, respectively, and the reducing powers ($OD_{700}$) were 0.02-0.17 and 0.07-1.90, respectively, at concentrations of 1-20 mg/mL. Thus, the HWE reducing power was markedly lower than that of the EE, but the SOD-like activity of the EE was significantly higher than that of the HWE. The nitrite-scavenging activities of HWE and EE were 9.25-19.18% and 11.94-53.49%, respectively, at concentrations of 1-20 mg/mL. Additionally, the TBARS (Thiobarbituric acid reactive substances, % value) of the EE (1-20 mg/mL) was 9.18-66.59%, thus 1.9-2.8-fold greater than that of the HWE (4.74-24.88%). In conclusion, we provide experimental evidence that extracts of CWGR may be natural antioxidants.

Analysis of major ginsenosides in various ginseng samples

  • Lee, Dong Gu;Lee, Ju Sung;Kim, Kyung-Tack;Kim, Hyun Young;Lee, Sanghyun
    • Journal of Applied Biological Chemistry
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    • v.62 no.1
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    • pp.87-91
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    • 2019
  • The contents of major ginsenosides (ginsenosides Rb1, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, and ginsenoside Rg1) in ginseng cultivated in different areas in Korea, ginseng that underwent different cultivation processes and ages, and ginseng cultivated in different countries were determined using high-performance liquid chromatography equipped with UV/VIS detector. Ginsenoside Rc was the most abundant ginsenoside in all different ginseng samples. The highest total concentration of major ginsenosides was found in the ginseng cultivated in Jinan (0.931 mg/g) and 4-year grown red ginseng (1.785 mg/g). Major ginsenosides were the most abundant in Korean ginseng (1.264 mg/g), compared to those in Chinese and American ginseng. The results of this study showed the different contents of major ginsenosides in the ginseng samples tested and emphasized which sample could contain high yield of ginsenosides.

Changes in Contents of Ginsenoside Due to Boiling Process of Panax ginseng C.A. Mayer

  • Sung, In Je;Ghimeray, Amal Kumar;Chang, Kwang Jin;Park, Cheol Ho
    • Korean Journal of Plant Resources
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    • v.26 no.6
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    • pp.726-730
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    • 2013
  • The purpose of the study was to determine a method to use fresh white Korean ginseng in the form of higher intake of medicinal components. Decoction was made at $70^{\circ}C$ and $90^{\circ}C$ in different intervals of time. HPLC (DAD) system was employed to monitor the ginsenosides content in the decoctions and the components were identified by comparing the retention time with that of reference compounds. However, decoction made at $70^{\circ}C$ in 72 hrs possessed higher amount of total ginsenosides ($209.6{\mu}g/mL$) content where considerable amount of bioactive ginsenosides like Rg3, Rb2, Rb1 and Rg1 were accumulated. Overall, it can be concluded that the fresh white Korean ginseng decoction made in 72 hrs at $70^{\circ}C$ would be useful for the health and other medicinal approach of ginseng.

Effects of pH and High Temperature Treatment on the Changes of Major Ginsenosides Composition in Korean Red Ginseng Water Extract (pH 및 고온 열처리가 홍삼물추출물의 주종 사포닌 성분변화에 미치는 영향)

  • Choi, Keum-Hee;Kwak, Yi-Seong;Rhee, Man-Hee;Hwang, Mi-Sun;Kim, Seok-Chang;Park, Chae-Kyu;Han, Gyeong-Ho;Song, Kyung-Bin
    • Journal of Ginseng Research
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    • v.32 no.2
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    • pp.127-134
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    • 2008
  • This study was carried out to investigate the changes of ginsenosides composition in Korean red ginseng water extract (RGWE) after heated with high temperatures above $100^{\circ}C$. RGWEs were adjusted with pH 3.0, pH 7.0 and pH 10.0, respectively, and then heated at 100,110 and $120^{\circ}C$ for 30 minutes by using autoclave. Total ginsenosides of RGWE treated with heating showed decreasing tendency when compared with control. By TLC analysis, decreasing effect of ginsenosides in RGWE were significantly observed in the acidic condition of pH 3.0, particulary. By HPLC analysis, total ginsenoside of control showed 1.89%, while those of RGWE treated with 100, 110 and $120^{\circ}C$ showed 1.22, 1.05 and 0.97%, respectively. The ratio of protopanaxadiol (PD) to protopanaxatriol (Pr) saponins in control was 1.89, while that of PD/PT in treated RGWEs were level of 1.33 to 1.47. By the result of decreased ratio of PD/PT in RGWE, it was considered that PD type saponin such as ginsenoside$-Rb_{1}$, $-Rb_{1}$, -Rc and -Rd was more unstable than PT type saponin such as ginsenoside-Re and Rg against high temperature heating above $100^{\circ}C$.