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http://dx.doi.org/10.7783/KJMCS.2020.28.6.445

Enhancement and Conversion of Ginsenoside Contents in Cultured Wild Ginseng Adventitious Root  

Kim, Chul Joong (Research Institute of Biotechnology, HwajinBioCosmetic)
Choi, Jae Hoo (Research Institute of Biotechnology, HwajinBioCosmetic)
Oh, Yeong Seon (Research Institute of Biotechnology, HwajinBioCosmetic)
Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University)
Lim, Jung Dae (Department of Bio-Health Convergence, Kangwon National University)
Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University)
Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic)
Publication Information
Korean Journal of Medicinal Crop Science / v.28, no.6, 2020 , pp. 445-454 More about this Journal
Abstract
Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.
Keywords
Panax ginseng C. A. Meyer; Cultured Wild Ginseng Adventitious Root; Elicitor; Ginsenoside; Lactic Acid Bacteria; Methyl Jasmonate;
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Times Cited By KSCI : 18  (Citation Analysis)
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