• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.595-599
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• 2013

The coordination of pyridyl-N to pentacyanoferrate for the detection of small organic antigens in solution is presented. The unique contribution of this paper is the direct conjugation of pyridyl-N in small organic antigens to pentacyanoferrate. Pentacyanoferrate is promising as an electrochemical label owing to its good electro-chemical properties, which can be utilized to generate an electrical signal in homogeneous electrochemical immunoassays. The facilely synthesized pyridyl-N to pentacyanoferrate was characterized by the electrochemical and spectroscopic methods. Hippuric acid (HA) has been detected competitively on the interaction of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA) to its antibody, with the detection limit of 0.50 ${\mu}g\;mL^{-1}$. While pentacyanoferrate-based immunoassay is in its simplicity and infancy, the proposed immunoassay offers attractive opportunities for developing pyridyl-N-based the electrochemical detection of small organic antigens in the health care area.

• 전기화학회지
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• 제17권3호
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• pp.172-178
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• 2014

이 논문에서는 체내에 존재하는 작은 유기물의 하나인 마뇨산을 빠르고 정량적으로 검출하기 위하여 전기화학적 면역분석법을 이용하였다. 마뇨산은 톨루엔이 인체에 노출되었을 때의 주된 신진 대사 물질로서 대표적인 생화학적 지표물질이다. 톨루엔 노출에 대한 신속하고 정확한 관찰은 산업현장에서 건강관리를 위하여 매우 중요하다. 그래서 마뇨산을 빠르고 정확하게 검출하기 위하여 전기화학적 면역분석법을 실시하였다. 본 연구에서 제시하는 새로운 면역분석방법은 전기화학적 활성물질의 하나인 도파민을 측정물질인 마뇨산과 직접 공유결합을 통하여 항원이 포함된 복합체를 합성하였다. 전기화학적 측면에서 도파민은 두 개의 하이드록시기를 가지고 있어 매우 뛰어난 산화/환원 신호를 보인다. 또한 도파민-마뇨산 복합물도 뛰어난 산화/환원 신호를 보이기 때문에 면역 분석에 적합하다. 도파민-마뇨산 복합물과 마뇨산의 항체와의 균질경쟁반응을 전기화학적인 방법을 통하여 관찰하였다. 본 면역분석을 통하여 실시한 결과는 마뇨산의 농도가 0.010~2.500 mg/mL 까지 정량적으로 분석됨으로써 실제 면역 센서에 적용할 수 있음을 확인하였다.

• 한국전기화학회:학술대회논문집
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• 한국전기화학회 2005년도 춘계총회 및 학술발표회
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• pp.30-30
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• 2005

• Bulletin of the Korean Chemical Society
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• 제33권5호
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• pp.1485-1490
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• 2012

An electrochemical immunoassay based on osmium-hippuric acid (HA) conjugate films onto the electrode is presented for the detection of urinary HA. This is the first report on the use of the oxidative electropolymerization of 5-amino-1,10-phenanthroline (5-$NH_2$-phen) for immobilizing an antigen, osmium-conjugated HA. As a redox mediator, [Os(5-amino-1,10-phenanthroline)$_2$(4-aminomethylpyridine-HA)Cl]$^{+/2+}$ (Os-phen-HA) was successfully synthesized and electropolymerized onto the screen-printed carbon electrodes (SPCEs). The interaction between osmium-HA conjugate films and antibody-HA ($anti$-HA) was performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The electrical signals were linearly proportional to urinary HA in the range of 0.1-5.0 mg/mL, which is sufficient for use as an immunosensor using a cutoff concentration of 2.0 mg/mL in urine samples. The proposed electrochemical immunoassay method can be extended to various applications for detecting a wide range of different small antigens in the health care area.

• Journal of Electrochemical Science and Technology
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• 제2권1호
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• pp.57-61
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• 2011

This paper describes an electrochemical immunoassay for simple, fast and quantitative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The electrochemical system of immunoassay was based on the directly osmium complex conjugated with hippuric acid. With the competition between free hippuric acid (HA) and the osmium-hippuric acid conjugate (Os-HA) to bind with antibody hippuric acid (Anti-HA) coated onto gold nanoparticles, the electrical signals were proportional to urinary hippuric acid (HA) in the range of 0.01-5 mg/mL which is enough range to be used for in-field or point-of-care (POC) diagnosis. The proposed electrochemical method can be extended to the applications to detect a wide range of different small molecules in the field of health care.

• 전기화학회지
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• 제10권3호
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• pp.229-232
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• 2007

An amperometric immunosensor for the determination of rabbit IgG is proposed. The immunoassay utilizes a screen-printed carbon electrode on which osmium redox polymer is electrodeposited. This immunoassay detects 0.1 ng/ml of rabbit IgG, which is ${\sim}10^2$ fold higher than the most sensitive enzyme amplified amperometric immunoassay. The assay utilizes a screen-printed carbon electrode which was pre-coated by a co-electrodeposited film of an electron conducting redox hydrogel and a rabbit IgG. The rabbit IgG in the electron conducting film conjugates captures, when present, the anti-rabbit IgG. The captured anti-rabbit-IgG is labeled with horseradish peroxidase (HRP) which catalyzes the two-electron reduction of $H_2O_2$ to water. Because the redox hydrogel electrically connects HRP reaction centers to the electrode, completion of the sandwich converts the film from non-electrocatalytic to electro-catalytic for the reduction of $H_2O_2$ to $H_2O$ when the electrode is poised at 200 mV vs. Ag/AgCl.

• Bulletin of the Korean Chemical Society
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• 제30권4호
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• pp.955-958
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• 2009

• 전기화학회지
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• 제14권1호
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• pp.44-49
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• 2011

This paper describes an electrochemical immunosensor for simple, fast and quantitative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The feature of this electrochemical system for immunoassay of hippuric acid is based on the direct conjugation of ferrocene to a hippuric acid. With the competition between the ferrocene-hippuric acid complex and hippuric acid for binding to the anti-hippuric acid monoclonal antibody coated onto gold nanoparticles, the electrical signals are turned out to be proportional to urinary hippuric acid in the range of 0.01-10 mg/mL, which is enough to be used for the point-of-care. The proposed electrochemical method could extend its applications to detect a wide range of different small molecules of antigens in the health care area.

• KSBB Journal
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• 제20권2호
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• pp.123-128
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• 2005

효율적인 바이오칩을 개발을 위해 칩 표면에 생체 물질들의 상호반응이 효과적으로 일어날 수 있는 센싱 표면의 조성과 항원-항체 반응과 같은 생체인식 반응을 정량적 신호로 전환하는 방법에 대해 연구하였다. 전기화학식 센서의 표면을 개선하기 위해 폴리아미도아민 덴드리머를 가교 물질로 도입하였다. 생체 분자들의 인식작용을 정량적인 신호로 전환하기 위해 전형적인 면역조직화학분석에서 사용된 반응들을 바이오센서에 적용한 방법론을 사용하였다 효소에 의해 촉매되는 신호화 방법은 면역반응들에 대하여 광학식 센서와 전기화학식 센서에서 공히 수행되었으며, 매우 정량적인 신호로 측정되었다. 측정된 신호들로부터 단백질 농도에 비례하는 검량곡선을 획득할 수 있었으며 다양한 면역 샘플에 대한 적용 가능성을 제시하였다.

• KSBB Journal
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• 제17권2호
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• pp.121-136
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• 2002

Immunosensors are of great interest because of their potential utility as specific, simple, label-free, direct detection means and provision of reduction in size, cost and time of analysis comparing with conventional immunoassay. In the last two decades, many reports have been published on the use of immunosensors for a wide range of applications to clinical diagnostics, pharmaceutical chemistry, environmental monitoring, biotechnology and food industries. There are also numerous transduction techniques developed such as electrochemical techniques, piezoelectric crystal, and surface plasmon resonance receiving much attention for the direct monitoring of immune reactions at solid surfaces. In this article, the principles, characteristics, structures, fonctions and clinical applications of immunosensors were reviewed