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http://dx.doi.org/10.5229/JKES.2014.17.3.172

Electrochemical Immunoassay based on the Dopamine-antigen Conjugate for Detecting Hippuric Acid  

Choi, Young-Bong (Department of chemistry, College of Advanced Science, Dankook University)
Jeon, Won-Yong (Department of Nanobiomedical Science & WCU Research Center, Dankook University)
Kim, Hyug-Han (Department of chemistry, College of Advanced Science, Dankook University)
Publication Information
Journal of the Korean Electrochemical Society / v.17, no.3, 2014 , pp. 172-178 More about this Journal
Abstract
In this work, we describe an electrochemical immunosensor for simple, fast and quantitative detection of a urinary hippuric acid (HA). Urinary HA, of molecular weight 180 DA, is one of the major metabolites and biological indicators in toluene-exposed humans. Simple and ubiquitous monitoring of exposure to toluene is very important in occupational health care. We propose the electrochemical immunoassay based on the dopamine-antigen conjugate for detecting hippuric acid. Our electrochemical immunoassay system employs a conjugate of dopamine (DA) as an electrochemical active molecule and hippuric acid (HA) as an antigen. As an electrochemical aspect, dopamine (DA) containing two hydroxyl group can show excellent redox signal. Also, dopamine-tethered hippuric acid (DA-HA) shows the reversible redox signal in the immunoassay. The competition between HA and DA-HA generated electric signals proportional to HA concentration. The electrochemical immunoassay was performed with DA-HA on the screen printed carbon electrodes (SPCEs), and then applies the mixture antigen (HA) and HA-antibody. The electrical signals were proportional to HA in the range of 0.010~2.500 mg/mL which is enough range to be used for the point-of-care.
Keywords
Hippuric acid; Dopamine; Electrochemical immunoassay;
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