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http://dx.doi.org/10.5012/bkcs.2012.33.5.1485

Electrochemical Immunoassay for Detecting Hippuric Acid Based on the Interaction of Osmium-Antigen Conjugate Films with Antibody on Screen Printed Carbon Electrodes  

Choi, Young-Bong (Department of Chemistry, Dankook University)
Jeon, Won-Yong (Department of Chemistry, Dankook University)
Kim, Hyug-Han (Department of Chemistry, Dankook University)
Publication Information
Bulletin of the Korean Chemical Society / v.33, no.5, 2012 , pp. 1485-1490 More about this Journal
Abstract
An electrochemical immunoassay based on osmium-hippuric acid (HA) conjugate films onto the electrode is presented for the detection of urinary HA. This is the first report on the use of the oxidative electropolymerization of 5-amino-1,10-phenanthroline (5-$NH_2$-phen) for immobilizing an antigen, osmium-conjugated HA. As a redox mediator, [Os(5-amino-1,10-phenanthroline)$_2$(4-aminomethylpyridine-HA)Cl]$^{+/2+}$ (Os-phen-HA) was successfully synthesized and electropolymerized onto the screen-printed carbon electrodes (SPCEs). The interaction between osmium-HA conjugate films and antibody-HA ($anti$-HA) was performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The electrical signals were linearly proportional to urinary HA in the range of 0.1-5.0 mg/mL, which is sufficient for use as an immunosensor using a cutoff concentration of 2.0 mg/mL in urine samples. The proposed electrochemical immunoassay method can be extended to various applications for detecting a wide range of different small antigens in the health care area.
Keywords
Electrochemical immunoassay; Hippuric acid; Osmium complex; Electropolymerization;
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