• Reproductive and Developmental Biology
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• 제32권1호
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• pp.21-25
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• 2008

Bone marrow mesenchymal stem cells (BMMSCs) have the capacity for self-renewal and differentiation into a variety of cell types. They represent an attractive source of cells for gene and cell therapy. The purpose of this study is to direct the specific expression of the DsRed reporter gene in $Sca-1^+$ BMMSCs differentiated into a cardiomyogenic lineage. We constructed the prMLC-2v-DsRed vector expressing DsRed under the control of the 309 tp fragment of the rat MLC-2v 5'-flanking region. The specific expression of the DsRed reporter gene under the transcriptional control of the 309 bp fragment of the rat MLC-2v promoter was tested in 5-azacytidine healed-$Sca-1^+$ BMMSCs over 2 weeks after the prMLC-2v-DsRed transfection. The prMLC-2v-DsRed was specifically expressed in the $Sca-1^+$ BMMSCs with cardiomyogenic lineage differentiation and it demonstrates that the 309 bp sequences of the rat MLC-2v 5'-flanking region is sufficient to confer cardiac specific expression on a DsRed reporter gene. The cardiac-specific promoter-driven reporter vector provides an important tool for the study of stem cell differentiation and cell replacement therapy in ischemic cardiomyopathy.

• Biomolecules & Therapeutics
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• 제26권4호
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• pp.380-388
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• 2018

Neural stem cells (NSCs) have the ability to self-renew and differentiate into multiple nervous system cell types. During embryonic development, the concentrations of soluble biological molecules have a critical role in controlling cell proliferation, migration, differentiation and apoptosis. In an effort to find optimal culture conditions for the generation of desired cell types in vitro, we used a microfluidic chip-generated growth factor gradient system. In the current study, NSCs in the microfluidic device remained healthy during the entire period of cell culture, and proliferated and differentiated in response to the concentration gradient of growth factors (epithermal growth factor and basic fibroblast growth factor). We also showed that overexpression of ASCL1 in NSCs increased neuronal differentiation depending on the concentration gradient of growth factors generated in the microfluidic gradient chip. The microfluidic system allowed us to study concentration-dependent effects of growth factors within a single device, while a traditional system requires multiple independent cultures using fixed growth factor concentrations. Our study suggests that the microfluidic gradient-generating chip is a powerful tool for determining the optimal culture conditions.

• 대한의용생체공학회:의공학회지
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• 제31권2호
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• pp.87-93
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• 2010

Researches for stem cells have been focused on scientists in biomedical sciences as well as clinical application for its great therapeutic potentials. Stem cells have two distinct characteristics: self-renewal and differentiation. In this short review, the links between stem cell research and biomedical engineering is discussed based on the basic characteristics of stem cells. This concept can be extended to the fundamental questions of biological sciences for cells such as proliferation, apoptosis, differentiation, and migration. For understanding proliferation and apoptosis of stem cells, techniques from biomedical engineering such as surface patterning, MEMS, nanotechnologies have been used. The advanced technologies such as microfluidic technologies, three dimensional scaffold fabrication, and mechanical/electrical stimulation have also been used in cell differentiation and migration. Basic and unsolved questions in the stem cell research field have limitations by studying conventional technologies. Therefore, the strategic fusion between stem cell biology and novel biomedical engineering field will break the barriers for understanding fundamental questions of stem cells, which can open the window for the clinical applications of stem cell based therapeutics as well as regeneration of damaged tissues.

• 한국실내디자인학회논문집
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• 제27권2호
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• pp.175-185
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• 2018

The purpose of this study is to analyze the concept of 'Gradual Differentiation' in parametric design in terms of pure model logic and thus describe the distinctive feature from the previous design method. To meet the purpose, it explores external cases like gradual factor identified in natural phenomenon and artworks and define the inherent model principles into "Self-similarity', "Correlation', and 'Temporality' by examining these features in terms of algorithm. Meanwhile, it identified the principle of gradual model representation in parametric design within a single system called 'Attractor System' by applying these three concepts into specific methods of parametric design, and by interpreting the logical structure through the association among 'Attractor', 'Field', and 'Differentiation'. The creative utilization of parameter shows that gradual model process in parametric design does not mean a passive "conversion process" merely replacing natural parameter with algorithm; rather, it refers to an active "generating process" creating new meanings and value. By continuing this process of conceptual understanding and insight, creative perspective and practical ability to interpret parameter can be improved.

• 생약학회지
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• 제46권1호
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• pp.52-58
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• 2015

Neural stem cells (NSCs), with self-renewal and neuronal differentiation capacity, are a feasible resource in cell-based therapies for various neurodegenerative diseases and neural tissue injuries. In this study, we investigated the effects of Schisandrae Fructus (SF) on proliferation and differentiation of human embryonic NSCs. Treatment with 70% ethanol extract of SF increased the viability of NSCs derived from human embryonic stem cells, which was accompanied by increased mRNA expression of cyclin D1. Whereas 70% ethanol extract of SF also decreased the mRNA expression of nestin, it increased class III ${\beta}$-tublin (Tuj-1) and MAP2 in both growth and differentiation media. Lastly, we found increased mRNA expression of BDNF in SF-treated NSCs. In conclusion, our study demonstrates for the first time that SF induced proliferation and neuronal differentiation of NSCs and increased mRNA expression of BDNF, suggesting its potential as a regulator of NSC fate in NSC-based therapy for neuronal injuries from various diseases.

• Asian-Australasian Journal of Animal Sciences
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• 제28권12호
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• pp.1721-1728
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• 2015

Embryonic stem cells (ESCs) have been used as a powerful tool for research including gene manipulated animal models and the study of developmental gene regulation. Among the critical regulatory factors that maintain the pluripotency and self-renewal of undifferentiated ESCs, NANOG plays a very important role. Nevertheless, because pluripotency maintaining factors and specific markers for livestock ESCs have not yet been probed, few studies of the NANOG gene from domestic animals including bovine have been reported. Therefore, we chose mouse ESCs in order to understand and compare NANOG expression between bovine, human, and mouse during ESCs differentiation. We cloned a 600 bp (-420/+181) bovine NANOG 5'-flanking region, and tagged it with humanized recombinant green fluorescent protein (hrGFP) as a tracing reporter. Very high GFP expression for bovine NANOG promoter was observed in the mouse ESC line. GFP expression was monitored upon ESC differentiation and was gradually reduced along with differentiation toward neurons and adipocyte cells. Activity of bovine NANOG (-420/+181) promoter was compared with already known mouse and human NANOG promoters in mouse ESC and they were likely to show a similar pattern of regulation. In conclusion, bovine NANOG 5-flanking region functions in mouse ES cells and has characteristics similar to those of mouse and human. These results suggest that bovine gene function studied in mouse ES cells should be evaluated and extrapolated for application to characterization of bovine ES cells.

• 가정과삶의질연구
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• 제27권3호
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• pp.93-114
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• 2009

The purposes of the study were to find the characteristics of spousal conflict, the characteristics of the origin of family in couple, the intervention techniques of the family therapist, and the processes of change in couple through family therapy. The total family counseling was 9 sessions, which included individual and couple therapy. The study used constant comparative analysis by using open coding method to find the factors. The study suggested the whole model for the study, which could be shown the processes of family therapy in this case study, by using graphical network display. The results of study showed the personal characteristics of husband and wife, the characteristics of the origin of family in couple, the intervention techniques of the family therapist, and the processes of change in husband through family therapy by using matrix. The results revealed that there were many differences in characteristics to cause spousal conflicts and characteriscts in family of origin to influence the crisis of divorce. The therapeutic intervention to treat the couples in the crisis of divorce were facilitation of communication between husband and wife, husband's differentiation of self from family of origin, and understanding about the perspectives of family members. As results of therapeutic intervention, there were showed the spousal change of recognition to each other, husband's differentiation of self, wife's growth of self, and improvement of communication.

• 한국항공운항학회지
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• 제23권3호
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• pp.94-104
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• 2015

Recently the departments of aviation-related in the college are increased as the aviation industry is rapidly expanded. To enhance their competitiveness and have differentiation, it is emphasized for each department to manage and teach their students well. Therefore this study aims to examine self-efficacy and academic adjustment of aviation-related college students and investigate the relationship between self-efficacy and academic adjustment. To achieve the objectives, it conducted a survey of aviation-related college students in C university from Apr. 27th. 2015 to May. 22th. 2015. A total of 225 questionnaires were analyzed using SPSS 18.0 statistical package program and frequency analysis, factor analysis, reliability analysis, multiple regression analysis, correlation analysis and one-way ANOVA were conducted. The result released that general self-efficacy was recognized higher at the department of aviation operation than aviation maintenance and aviation service and social self-efficacy was recognized higher at the department of aviation service than aviation maintenance and aviation operation. Also general self-efficacy had an influence on the motivation, application and performance and social self-efficacy affected motivation, performance and academic environment. The implication of these findings would help to manage aviation-related college students, develop their departments and provide the directions for future research.

• Molecules and Cells
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• 제39권8호
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• pp.619-624
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• 2016

Glioblastoma stem cells (GBM-SCs) are believed to be a subpopulation within all glioblastoma (GBM) cells that are in large part responsible for tumor growth and the high grade of therapeutic resistance that is so characteristic of GBM. MicroRNAs (miR) have been implicated in regulating the expression of oncogenes and tumor suppressor genes in cancer stem cells, including GBM-SCs, and they are a potential target for cancer therapy. In the current study, miR-203 expression was reduced in $CD133^+$ GBM-SCs derived from six human GBM biopsies. MicroRNA-203 transfected GBM-SCs had reduced capacity for self-renewal in the cell sphere assay and increased expression of glial and neuronal differentiation markers. In addition, a reduced proliferation rate and an increased rate of apoptosis were observed. Therefore, miR-203 has the potential to reduce features of stemness, specifically in GBM-SCs, and is a logical target for GBM gene therapy.

• Molecules and Cells
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• 제45권11호
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• pp.820-832
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• 2022

As a potential candidate to generate an everlasting cell source to treat various diseases, embryonic stem cells are regarded as a promising therapeutic tool in the regenerative medicine field. Cohesin, a multi-functional complex that controls various cellular activities, plays roles not only in organizing chromosome dynamics but also in controlling transcriptional activities related to self-renewal and differentiation of stem cells. Here, we report a novel role of the α-kleisin subunits of cohesin (RAD21 and REC8) in the maintenance of the balance between these two stem-cell processes. By knocking down REC8, RAD21, or the non-kleisin cohesin subunit SMC3 in mouse embryonic stem cells, we show that reduction in cohesin level impairs their self-renewal. Interestingly, the transcriptomic analysis revealed that knocking down each cohesin subunit enables the differentiation of embryonic stem cells into specific lineages. Specifically, embryonic stem cells in which cohesin subunit RAD21 were knocked down differentiated into cells expressing neural alongside germline lineage markers. Thus, we conclude that cohesin appears to control the fate determination of embryonic stem cells.