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Effects of Schisandrae Fructus 70% Ethanol Extract on Proliferation and Differentiation of Human Embryonic Neural Stem Cells  

Baral, Samrat (From the BK21 Plus team)
Pariyar, Ramesh (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Yoon, Chi-Su (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Yun, Jong-Min (Department of Internal Medicine, College of Korean Medicine, Wonkwang University)
Jang, Seok O (Department of Internal Medicine, College of Korean Medicine, Wonkwang University)
Kim, Sung Yeon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Oh, Hyuncheol (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (From the BK21 Plus team)
Seo, Jungwon (From the BK21 Plus team)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.1, 2015 , pp. 52-58 More about this Journal
Abstract
Neural stem cells (NSCs), with self-renewal and neuronal differentiation capacity, are a feasible resource in cell-based therapies for various neurodegenerative diseases and neural tissue injuries. In this study, we investigated the effects of Schisandrae Fructus (SF) on proliferation and differentiation of human embryonic NSCs. Treatment with 70% ethanol extract of SF increased the viability of NSCs derived from human embryonic stem cells, which was accompanied by increased mRNA expression of cyclin D1. Whereas 70% ethanol extract of SF also decreased the mRNA expression of nestin, it increased class III ${\beta}$-tublin (Tuj-1) and MAP2 in both growth and differentiation media. Lastly, we found increased mRNA expression of BDNF in SF-treated NSCs. In conclusion, our study demonstrates for the first time that SF induced proliferation and neuronal differentiation of NSCs and increased mRNA expression of BDNF, suggesting its potential as a regulator of NSC fate in NSC-based therapy for neuronal injuries from various diseases.
Keywords
Schisandrae Fructus; Human Embryonic Neural Stem Cells; Proliferation; Neuronal Differentiation; BDNF;
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