• 대한의생명과학회지
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• 제24권4호
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• pp.430-434
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• 2018

Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.

• 대한수학회지
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• 제48권1호
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• pp.49-61
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• 2011

Let D be an integral domain with quotient field K, X be a nonempty set of indeterminates over D, * be a star operation on D, $N_*$={f $\in$ D[X]|c(f)$^*$= D}, $*_w$ be the star operation on D defined by $I^{*_w}$ = ID[X]${_N}_*$ $\cap$ K, and [*] be the star operation on D[X] canonically associated to * as in Theorem 2.1. Let $A^g$ (resp., $A^{[*]g}$, $A^{[*]g}$) be the global (resp.,*-global, [*]-global) transform of a ring A. We show that D is a $*_w$-Noetherian domain if and only if D[X] is a [*]-Noetherian domain. We prove that $D^{*g}$[X]${_N}_*$ = (D[X]${_N}_*$)$^g$ = (D[X])$^{[*]g}$; hence if D is a $*_w$-Noetherian domain, then each ring between D[X]${_N}_*$ and $D^{*g}$[X]${_N}_*$ is a Noetherian domain. Let $\tilde{D}$ = $\cap${$D_P$|P $\in$ $*_w$-Max(D) and htP $\geq$2}. We show that $D\;\subseteq\;\tilde{D}\;\subseteq\;D^{*g}$ and study some properties of $\tilde{D}$ and $D^{*g}$.

• 충청수학회지
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• 제24권3호
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• pp.601-607
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• 2011

Let D be an integral domain, X be an indeterminate over D, and D[X] be the polynomial ring over D. We show that D is an almost weakly factorial PvMD if and only if D + XDS[X] is an integrally closed almost GCD-domain for each (saturated) multiplicative subset S of D, if and only if $D+XD_1[X]$ is an integrally closed almost GCD-domain for any t-linked overring $D_1$ of D, if and only if $D_1+XD_2[X]$ is an integrally closed almost GCD-domain for all t-linked overrings $D_1{\subseteq}D_2$ of D.

• 대한수학회지
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• 제53권2호
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• pp.447-459
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• 2016

Let D be an integral domain, {$X_{\alpha}$} be a nonempty set of indeterminates over D, and $D{\mathbb{[}}\{X_{\alpha}\}{\mathbb{]}_1}$ be the first type power series ring over D. We show that if D is a t-SFT $Pr{\ddot{u}}fer$ v-multiplication domain, then $D{\mathbb{[}}\{X_{\alpha}\}{\mathbb{]}}_{1_{D-\{0\}}}$ is a Krull domain, and $D{\mathbb{[}}\{X_{\alpha}\}{\mathbb{]}}_1$ is a $Pr{\ddot{u}}fer$ v-multiplication domain if and only if D is a Krull domain.

• 대한수학회보
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• 제52권2호
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• pp.525-530
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• 2015

Let D be an integrally closed domain with quotient field K, X be an indeterminate over D, $f=a_0+a_1X+{\cdots}+a_nX^n{\in}D[X]$ be irreducible in K[X], and $Q_f=fK[X]{\cap}D[X]$. In this paper, we show that $Q_f$ is a maximal ideal of D[X] if and only if $(\frac{a_1}{a_0},{\cdots},\frac{a_n}{a_0}){\subseteq}P$ for all nonzero prime ideals P of D; in this case, $Q_f=\frac{1}{a_0}fD[X]$. As a corollary, we have that if D is a Krull domain, then D has infinitely many height-one prime ideals if and only if each maximal ideal of D[X] has height ${\geq}2$.

• Molecules and Cells
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• 제20권3호
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• pp.446-451
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• 2005

Diap1 is an essential Drosophila cell death regulator that binds to caspases and inhibits their activity. Reaper, Grim and Hid each antagonize Diap1 by binding to its BIR domain, activating the caspases and eventually causing cell death. Reaper and Hid induce cell death in a Ring-dependent manner by stimulating Diap1 auto-ubiquitination and degradation. It was not clear that how Grim causes the ubiquitination and degradation of Diap1 in Grim-dependent cell death. We found that Grim stimulates poly-ubiquitination of Diap1 in the presence of UbcD1 and that it binds to UbcD1 in a GST pull-down assay, so presumably promoting Diap1 degradation. The possibility that dBruce is another E2 interacting with Diap1 was examined. The UBC domain of dBruce slightly stimulated poly-ubiquitination of Diap1 in Drosophila extracts but not in the reconstitution assay. However Grim did not stimulate Diap1 poly-ubiquitination in the presence of the UBC domain of dBruce. Taken together, these results suggest that Grim stimulates the poly-ubiquitination and presumably degradation of Diap1 in a novel way by binding to UbcD1 but not to the UBC domain of dBruce as an E2.

• 한국자기공명학회논문지
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• 제17권1호
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• pp.59-66
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• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• 대한수학회보
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• 제48권6호
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• pp.1137-1146
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• 2011

Let {$X_i$, $i{\geq}1$} be a sequence of i.i.d. nondegenerate random variables which is in the domain of attraction of the normal law with mean zero and possibly infinite variance. Denote $S_n={\sum}_{i=1}^n\;X_i$, $M_n=max_{1{\leq}i{\leq}n}\;{\mid}S_i{\mid}$ and $V_n^2={\sum}_{i=1}^n\;X_i^2$. Then for d > -1, we showed that under some regularity conditions, $$\lim_{{\varepsilon}{\searrow}0}{\varepsilon}^2^{d+1}\sum_{n=1}^{\infty}\frac{(loglogn)^d}{nlogn}I\{M_n/V_n{\geq}\sqrt{2loglogn}({\varepsilon}+{\alpha}_n)\}=\frac{2}{\sqrt{\pi}(1+d)}{\Gamma}(d+3/2)\sum_{k=0}^{\infty}\frac{(-1)^k}{(2k+1)^{2d+2}}\;a.s.$$ holds in this paper, where If g denotes the indicator function.

• 한국통신학회논문지
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• 제28권5C호
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• pp.461-467
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• 2003

본 논문에서는 DCT 영역에서의 효과적인 분리 대칭 선형 필터링 방법을 제안한다. 먼저 2차원 분리 선형 필터링을 DCT 영역에서의 1차원 필터링이 연결된 형태로 분해한다. 필터 계수가 대칭적일 때, DCT 영역의 필터링행렬의 특별한 성질을 분석하였다. 이러한 성질을 이용하여 효과적인 DCT 영역에서의 2차원 필터링을 수행한다. 제안된 방법은 이전의 DCT 영역에서의 선형 필터링 방법에 비해서 일반적인 DCT 계수들이 가지고 있는 희소성을 고려할 때, 기존의 방법에 비하여 더 적은 수의 연산을 요구한다. 그리고 제안된 방식은 간단하고 규칙적인 연산 구조로 구성되어 있기 때문에, 효과적인 VLSI 구현에 적합하다.

• 한국수산과학회지
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• 제48권6호
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• pp.913-919
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• 2015

In vertebrates, the vitamin D receptor (VDR), a member of the nuclear receptor superfamily, binds the biologically active ligand $1{\alpha},25-(OH)_2$-vitamin $D_3$ (1,25 $D_3$). Nearly all vertebrates, including Agnatha, possess a VDR with high ligand selectivity for 1,25 $D_3$ and related metabolites. Although a putative ancestral VDR gene is present in the genome of the chordate invertebrate Ciona intestinalis, the functional characteristics of marine invertebrate VDR are still obscure. To elucidate the ascidian Halocynthia roretzi VDR (HrVDR), we cloned full-length HrVDR cDNA and investigated the transcriptional activity of HrVDR in HEK293 cells. HrVDR consists of 1,680 nucleotides (559 amino acids [aa]), including a short N-terminal region (A/B domain; 26 aa), DNA-binding domain (C domain; 72 aa), hinge region (D domain; 272 aa), and C-terminal ligand-binding domain (E domain; 161 aa). The amino acid sequence identity of HrVDR was greatest to that of C. intestinalis VDR (56%). In the luciferase reporter assays, the transcriptional activity of HrVDR was not significantly increased by 1,25 $D_3$, whereas the farnesoid X receptor agonist GW4064 increased the transactivation of HrVDR. These results suggest the presence of a novel ligand for and a distinct ligand-binding domain in ascidian VDR.