Browse > Article
http://dx.doi.org/10.5657/KFAS.2015.0913

Characterization and Transcriptional Activity of a Vitamin D Receptor Ortholog in the Ascidian Halocynthia roretzi  

Lee, Jung Hwan (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University)
Sohn, Young Chang (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.48, no.6, 2015 , pp. 913-919 More about this Journal
Abstract
In vertebrates, the vitamin D receptor (VDR), a member of the nuclear receptor superfamily, binds the biologically active ligand $1{\alpha},25-(OH)_2$-vitamin $D_3$ (1,25 $D_3$). Nearly all vertebrates, including Agnatha, possess a VDR with high ligand selectivity for 1,25 $D_3$ and related metabolites. Although a putative ancestral VDR gene is present in the genome of the chordate invertebrate Ciona intestinalis, the functional characteristics of marine invertebrate VDR are still obscure. To elucidate the ascidian Halocynthia roretzi VDR (HrVDR), we cloned full-length HrVDR cDNA and investigated the transcriptional activity of HrVDR in HEK293 cells. HrVDR consists of 1,680 nucleotides (559 amino acids [aa]), including a short N-terminal region (A/B domain; 26 aa), DNA-binding domain (C domain; 72 aa), hinge region (D domain; 272 aa), and C-terminal ligand-binding domain (E domain; 161 aa). The amino acid sequence identity of HrVDR was greatest to that of C. intestinalis VDR (56%). In the luciferase reporter assays, the transcriptional activity of HrVDR was not significantly increased by 1,25 $D_3$, whereas the farnesoid X receptor agonist GW4064 increased the transactivation of HrVDR. These results suggest the presence of a novel ligand for and a distinct ligand-binding domain in ascidian VDR.
Keywords
cDNA; Tunicate; Vitamin D receptor; Vitamin D responsive reporter gene; Halocynthia roretzi;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bouillon R and Suda T. 2014. Vitamin D: calcium and bone homeostasis during evolution. Bonekey Rep 3, 480. http://dx.doi.org/10.1038/bonekey.2013.214.
2 Holick MF. 2011. Vitamin D: evolutionary, physiological and health perspectives. Curr Drug Targets 12, 4-18.   DOI
3 Howarth DL, Law SH, Barnes B, Hall JM, Hinton DE, Moore L, Maglich JM, Moore JT and Kullman SW. 2008. Paralogous vitamin D receptors in teleosts: transition of nuclear receptor function. Endocrinology 149, 2411-2422.   DOI
4 Huelsenbeck JP, Ronquist F, Nielsen R and Bollback JP. 2001. Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294, 2310-2314.   DOI
5 Kim JY, Son YL and Lee YC. 2009. Involvement of SMRT corepressor in transcriptional repression by the vitamin D receptor. Mol Endocrinol 23, 251-264. http://dx.doi.org/10.1210/me.2008-0426.   DOI
6 Koszewski NJ, Herberth J and Malluche HH. 2010. Retinoic acid receptor gamma 2 interactions with vitamin D response elements. J Steroid Biochem Mol Biol 120, 200-207.   DOI
7 Landry CS, Ruppe MD and Grubbs EG. 2011. Vitamin D receptors and parathyroid glands. Endocr Pract 17, 63-68.   DOI
8 Maeng S, Lee JH, Kim GJ, Kim SH, Kwon HC, Shin YK and Sohn YC. 2012a. Molecular and expression analysis of the farnesoid X receptor in the urochordate Halocynthia roretzi. Comp Biochem Physiol B Biochem Mol Biol 161, 189-196.   DOI
9 Maeng S, Lee JH, Choi SC, Kim MA, Shin YK and Sohn YC. 2012b. The retinoid X receptor in a marine invertebrate chordate: evolutionary insights from urochordates. Gen Comp Endocrinol 178, 380-390.   DOI
10 Maglich JM, Caravella JA, Lambert MH, Willson TM, Moore JT and Ramamurthy L. 2003. The first completed genome sequence from a teleost fish (Fugu rubripes) adds significant diversity to the nuclear receptor superfamily. Nucleic Acids Res 31, 4051-4058.   DOI
11 Norman AW, Okamura WH, Bishop JE and Henry HL. 2002. Update on biological actions of $1{\alpha},25(OH)_{2}-vitamin\;D_3$ (rapid effects) and $24R,25(OH)_{2}-vitamin\;D_3$. Mol Cell Endocrinol 197, 1-13.   DOI
12 Park W, Kim GJ, Choi HS, Vanacker JM and Sohn YC. 2009. Conserved properties of a urochordate estrogen receptor-related receptor (ERR) with mammalian ERRalpha. Biochim Biophys Acta 1789, 125-134.   DOI
13 Raslan AA, Lee JH, Shin J, Shin YK and Sohn YC. 2013. Transcriptional activity and expression of liver X receptor in the ascidian Halocynthia roretzi. Zoolog Sci 30, 731-741.   DOI
14 Reschly EJ, Bainy AC, Mattos JJ, Hagey LR, Bahary N, Mada SR, Ou J, Venkataramanan R and Krasowski MD. 2007. Functional evolution of the vitamin D and pregnane x receptors. BMC Evol Biol 12, 7-222.
15 Sanchez-Martínez R, Castillo AI, Steinmeyer A and Aranda A. 2006. The retinoid X receptor ligand restores defective signalling by the vitamin D receptor. EMBO Rep 7, 1030-1034.   DOI
16 Suzuki T, Suzuki N, Srivastava AS and Kurokawa T. 2000. Identification of cDNAs encoding two subtypes of vitamin D receptor in flounder, Paralichthys olivaceus. Biochem Biophys Res Commun 270, 40-45.   DOI
17 Yagi K, Satou Y, Mazet F, Shimeld SM, Degnan B, Rokhsar D, Levine M, Kohara Y and Satoh N. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis. III. Genes for Fox, ETS, nuclear receptors and NFkappaB. Dev Genes Evol 213, 235-244.   DOI