• 대한방사선기술학회지:방사선기술과학
• /
• 제39권3호
• /
• pp.451-459
• /
• 2016

방사선 사고 및 방사선 치료 등 방사선에 의한 과피폭의 피해를 줄이기 위해 방사선방호제 개발연구는 활발히 진행되고 있다. 이에 화학적 합성물이 아닌 항산화, 항암, 면역 증강에 효과적인 것으로 알려진 백하수오를 이용하여 방사선방호효과를 확인하였다. 백하수오 에탄올추출물을 Sprague Dawley Rat (SD Rat)에 14일간 1일 1회 경구 투여하고, 7 Gy X-ray를 조사한 후 1일, 4일, 7일, 21일의 시간 변화에 따른 혈구성분, 비장 지수의 변화 및 간과 자궁의 조직변화를 관찰하였다. 실험결과 백하수오 에탄올추출물을 섭취한 실험군의 백혈구 수치(p < 0.05)와 비장 지수(p < 0.05)가 대조군보다 회복이 빠른 것을 확인하였다. 간 조직에서는 핵의 응축, 세포질의 팽창, 염증세포의 침윤이 감소하였으며, 자궁샘 조직은 세포고사가 감소한 것을 확인하였다. 위의 결과를 토대로 백하수오 에탄올추출물은 방사선 조사에 따른 혈구 및 장기의 피해를 줄일 수 있는 새로운 방사선방호제로써 유용할 것으로 기대되며, 방사선 사고와 같은 비상관리 분야에 적절한 시사점을 제공할 수 있다.

• 대한본초학회지
• /
• 제30권6호
• /
• pp.83-91
• /
• 2015

Objectives : The present study was conducted to examine whether Cynanchi wilfordii radix (CWR) with or without fermentation has an ameliorative effect on hyperlipidemia in rats.Methods : We analyzed the contents of Conduritol F on Cynanchi wilfordii radix. The experimental animals were divided into six groups; normal diet fed group (N), high cholesterol fed control group (Con), Lovastatin 20 mg/kg (L), CWR-W 300 mg/kg (CWR-W), and CWR-F 300 mg/kg on hyperlipidemia model induced by feeding 1.25% cholesterol. Rats were administrated orally every day for 8 weeks. And lipid profile of serum and weight change were observed.Results : The vehicle displayed a markedly increased body weight and significantly increased liver and epididymal fat weight, however, the administration of CWR improved the body, liver, and epididymal fat weights. All drug treatment reduced significantly the serum level of total cholesterol and LDL-cholesterol elevated by intake of high cholesterol diet. TG displayed a reducing tendency all drug treatment, however, CWR-W decreased significantly. Atherogenic index and cardiac risk factor increased high cholesterol diet fed control group, while the administration of CWR-W and CWR-F decreased significantly. The major index of liver injury such as AST and ALT improved in all drug treatment.Conclusions : These results suggest that CWR extended the effect of lipid enhanced. Therefore CWR with or without fermentation may be useful for therapeutic treatment of clinical conditions associated with hyperlipidemia. Finally, these require more investigations about the action mechanism of CWR in the future.

• 한국식품영양과학회지
• /
• 제44권4호
• /
• pp.564-572
• /
• 2015

본 연구는 백하수오가 가지는 일반 성분과 영양 성분에 대한 탐색을 실시하였다. 무기질에서 높은 칼륨 성분을 보이고 있었으며 라디칼 소거능 역시 우수한 것으로 나타났다. 아미노산에서는 유리 및 구성 아미노산 모두 arginine이 높은 함량을 나타냈으며 생리활성이 우수한 것으로 보고되는 ${\gamma}$-aminobutyric acid(GABA) 역시 높은 함량을 보이고 있다. 지방산 조성을 볼 때 필수지방산으로 알려진 linoleic acid가 가장 주된 지방산으로 나타났으며 ${\omega}$-3 계열인 linolenic acid가 존재하는 것으로 볼 때 우수한 영양적 조성을 가지고 있다고 볼 수 있다. 관능검사를 통해 백하수오차의 경우 녹차에 비해 약간의 쓴맛과 단맛이 부족하다고 측정되었으나 농도 조절이나 부재료 첨가를 통해 개선할 수 있을 것으로 판단된다.

• 생약학회지
• /
• 제24권2호
• /
• pp.166-176
• /
• 1993

This study was attempted to investigate the acute toxicity in mice, the subacute toxicity in rats of Cynanchi wilfordii Radix extract, the effect on transaminase activities, hematological parameters, leukocyte parameters in serum of subacute-toxicated rats. In acute toxicity test, the death rate was not observed in 50, 100, 200, 300 mg/kg(i.p.), one tenths to two tenths in 300, 500, 1000, 1500 mg/kg(p.o.) for two weeks. In subacute test, rats were all died in 300 mg/kg(p.o.) during 4 weeks, in 500, 1000 mg/kg(p.o.) during three weeks. The cause of death believed to be stomach ulcer. The activities of S-GOT and S-GPT were significantly increased in all sample-treated groups, when compared with the normal groups. A number of WBC and neutrophil belong to hematological parameter were significantly increased, lymphocyte was decreased in all sample-treated group, when compared with normal group. The hemolytic action on water extract, saponin and alcohol extract showed very low activities.

• Journal of Acupuncture Research
• /
• 제20권4호
• /
• pp.42-52
• /
• 2003

Objective: For designing an optimal countermeasure against ageing and ageing related disease, it is necessary to understand the ageing mechanism and other reactive species. Free radicals are associated with the mechanisam of biological ageing and age-related diseases. This study was performed to identifiy the scavenging effect of Radix Cynanchi Wilfordii Herbal-Acupunture Solution(RCWHA) on Nitric Oxide(NO), 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) and Interleukin-4(IL -4). Methods: The activity to scavenge DPPH radicals and NO was observed in vitro, We evaluated the antioxidant activity of RCWHA by using DPPH or NO free radical method. Also the inhibitory effect on IL-4 in RCWHA was observed in vitro, Results: The followings are the summary of the results; (1) There is a significant scavenging effect of RCWHA on NO in 0.1mg/ml and 10mg/ml group after 24hrs. (2) There is no significant scavenging effect of RCWHA on DPPH. (3) There is a significant scavenging effect of RCWHA on IL-4 in $10{\mu}g/m{\ell}$ and $100{\mu}g/m{\ell}$ group. Conclusions: These findings indicate that RCWHA can be used as antioxidant or antiimflamatory drug. But further study is needed about the effect of RCWHA.

• 생약학회지
• /
• 제49권1호
• /
• pp.55-64
• /
• 2018

Recently, it has been a big issue to distinguish the dried roots of Cynanchum wilfordii and C. auriculatum in health functional food market. The original plant species of Cynanchi Wilfordii Radix belong to the Asclepiadaceae family is differentially described in the national pharmacopoeia of Korea, China and Japan. Owing to the morphological similarities of the dried roots of this plant to those of C. auriculatum, which is often misidentified in Korean herbal medicine marketplace, distinguishing these two species is exceedingly difficult. The purpose of this study was to compare the conventional-PCR with the real-time PCR for detection of C. wilfordii and C. auriculatum DNA. We also tried to realize a quantitative real-time PCR assay using species-specific matK primers, which allowed us to estimate the ratio of C. willfordii and C. auriculatum using varying ratios of mixed genomic DNA template from the two species. The differentiation of intentional and unintentional mixture in this study would be applied to food safety management and can be helpful for protection of consumer's right and cultivators.

• 한국식품영양과학회지
• /
• 제43권12호
• /
• pp.1954-1958
• /
• 2014

본 연구에서는 백하수오 분말을 첨가(0, 2, 4, 6, 8%)하여 제조한 양갱의 물리적 품질 특성, 항산화 활성 및 기호도 검사를 실시하고 대조구와 비교하였다. 백하수오 분말 첨가량이 증가할수록 양갱의 색도 $L^*$, $a^*$, $b^*$ 값은 모두 증가하였고 경도는 대조구가 $1.10kg{\small{f}}$, 8% 첨가구가 $0.68kg{\small{f}}$로 감소하였다(P<0.05). pH는 대조구가 5.83, 8% 첨가구가 6.32로 가장 높게 나타나 백하수오 분말 첨가량이 증가할수록 유의적으로 증가하였으며(P<0.05), 수분함량 또한 전체적으로 증가하는 경향을 나타내었다. 한편 6% 첨가구를 제외한 0~8% 시료 간에 가용성 고형분($^{\circ}Brix$)에 유의적인 차이가 없었다(P>0.05). 소비자 기호도 평가에서 백하수오 분말첨가량이 증가할수록 색의 기호도는 유의적으로 감소하였고(P<0.05), 향은 8% 첨가구가 3.88로 가장 낮은 평가를 받았으며, 씹힘성은 2% 첨가구가 4.67로 가장 높은 평가를 받았다. 맛과 전체적인 기호도는 0~4% 시료 간에 유의적인 차이가 없었지만 대조구보다 2~4% 첨가구가 상대적으로 높은 평가를 받았으며 2% 첨가구가 각각 4.38, 4.88로 가장 높게 평가되었다.

• 대한본초학회지
• /
• 제34권2호
• /
• pp.49-58
• /
• 2019

Objectives : Obesity is a public health concern associated with chronic diseases including hyperlipidemia, diabetes, fatty liver, atherosclerosis and cancer. As several anti-obesity drugs have been limited owing to their side effects, the development of new anti-obesity drugs through herbal medicines has been increasing. Cynanchum Wilfordii Radix (CW) traditionally is consumed for various health benefits including immune enhancing, anti-inflammation and anti-tumor activities. The aim of the present study is to evaluate the effects of CW on High fat diet (HFD)-induced obese mice. Methods : The mice were randomly divided into four groups (n=7). The mice were respectively fed a normal diet (ND), a high-fat diet (HFD), HFD plus CW (50 mg/kg/day), HFD plus CW (100 mg/kg/day). All groups were assayed for body weights, food efficiency ratio, blood biochemistry parameters, and organic tissue weights. Results : HFD-fed mice showed an increase in the body weight and serum biochemistry parameters levels (total cholesterol and triglycerides) as well as organic tissue weights. However, the administration of CW to obese mice induced a reduction in their body weight, food efficiency ratio, blood biochemistry parameters and weight of liver and fat compared with the HFD fed mice. Additionally, we observed that CW inhibited the lipid accumulation in liver and serum lipid parameter induced by HFD. Conclusions : Taken together, the findings of this study suggest that CW may be a potential agent for use in the treatment of obesity and obesity-related metabolic diseases.

• 한국식품저장유통학회지
• /
• 제23권3호
• /
• pp.432-437
• /
• 2016

본 논문은 국내 적하수오 및 백하수오 추출물의 항산화능 및 항균활성을 구명하기 위한 목적으로 진행하였다. 각 하수오의 열수, 에탄올, 부탄올 추출물에 대하여, 일반성분을 분석한 후, 항산화능에 대해서는 총 페놀함량 및 DPPH 라디칼 소거능을 측정하였으며, 항균활성에 대해서는 Staphylococcus aureus 및 Propionibacterium acne에 대한 최소생육저해농도(minimum inhibitory concentration, MIC) 및 생육저해환을 통하여 평가하였다. 적하수오 및 백하수오 부탄올 추출물의 총 페놀함량은 각각 1,212.6 및 1,454.5 mg/g GAE로 다른 유기용매 추출물에 비해 유의적으로 높은 함량을 보였다. 이에 비해 DPPH 라디칼 소거능은 열수추출물에 비해 에탄올 추출물(89.0%), 부탄올 추출물(88.9%)이 유의적으로 높은 값을 나타내었다. MIC 측정 결과, 적 백하수오 에탄올 추출물만이 0.8 mg/mL로 S. aureus와 P. acne에 대해서 항균효과를 나타냈다. 디스크 확산법 측정 결과, 모든 유기용매 추출물이 5 mg/disc 농도부터 생육저해환을 형성하였으며, 농도가 증가함에 따라 생육저해환의 크기도 증가하였다. 적하수오 및 백하수오 에탄올 추출물의 조사균주에 대한 생육저해완의 직경은 10 mg/mL 농도에서 각각 8.9 및 9.2 mm를 나타내, 추출물 중 가장 우수한 항균활성을 나타냈다. 본 연구는 하수오 추출물이 항산화 효과 뿐만 아니라 피부 건강과 관련된 세균에 대해 항균활성을 보여줌으로써, 하수오 추출물이 피부 건강을 위한 천연 유래의 기능성 화장품 소재로도 활용될 수 있는 잠재적 가능성을 제시하였다.

• 동의생리병리학회지
• /
• 제31권6호
• /
• pp.374-379
• /
• 2017

Hair loss affects interpersonal relationships and causes psychological stress. In this study, we investigated the antioxidant activity of Cynanchi Wilfordii Radix (CWR) and its effects on dermal papilla (DP) cells. Antioxidant efficacy was examined by ABTS assay. To confirm the effect on cell activity, MTS assay was performed and cell count was directly measured by hemocytometer. The mRNA expression of genes involved in hair formation and hair loss formation was confirmed by quantitative RT-PCR. CWR has a strong antioxidant activity. Cell viability of DP cells was increased to 118.5% by treatment of 0.5 mg/ml CWR for 24 hours, but the effect on the cell number was insignificant. These results suggest that CWR increases mitochondrial activity without promoting cell proliferation. Treatment of DP cells with 0.5 mg/ml CWR resulted in 48.5% reduction of mRNA expression of type 2 $5{\alpha}$-reductase, a major cause of male hair loss. In addition, mRNA expression of bone morphogenetic pretein (BMP), fibroblast growth factor (FGF)7, and FGF10, which are closely related to hair growth, was also decreased. Reactive oxygen species (ROS) acts as a cause of hair loss. The excellent antioxidant efficacy of CWR is thought to be able to effectively remove ROS. The dihydrotestosterone produced by type 2 $5{\alpha}$-reductase in DP cells is a potent inducer of male pattern hair loss. The inhibitory effect of type 2 $5{\alpha}$-reductase mRNA on DP cells induced by CWR may induce a positive therapeutic effect of male pattern hair loss.