한국식품저장유통학회지 (Food Science and Preservation)

  • 제23권3호
  • /
  • Pages.432-437
  • /
  • 2016
  • /
  • 3022-5477(pISSN)
  • /
  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
권호 표지
DOI QR코드

DOI QR Code

적하수오 및 백하수오 추출물의 항산화 및 항균 활성

Antioxidant and antimicrobial activities of Jeok Hasuo (Polygonum multiflorum Thunb.) and Baek Hasuo (Cynanchi wilfordii Radix) root extracts

  • 최현경 (상명대학교 식물식품공학과) ;
  • 장유이 (에스에스바이오팜(주)) ;
  • 오준현 (상명대학교 식물식품공학과)
  • 투고 : 2016.05.25
  • 심사 : 2016.06.03
  • 발행 : 2016.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 논문은 국내 적하수오 및 백하수오 추출물의 항산화능 및 항균활성을 구명하기 위한 목적으로 진행하였다. 각 하수오의 열수, 에탄올, 부탄올 추출물에 대하여, 일반성분을 분석한 후, 항산화능에 대해서는 총 페놀함량 및 DPPH 라디칼 소거능을 측정하였으며, 항균활성에 대해서는 Staphylococcus aureus 및 Propionibacterium acne에 대한 최소생육저해농도(minimum inhibitory concentration, MIC) 및 생육저해환을 통하여 평가하였다. 적하수오 및 백하수오 부탄올 추출물의 총 페놀함량은 각각 1,212.6 및 1,454.5 mg/g GAE로 다른 유기용매 추출물에 비해 유의적으로 높은 함량을 보였다. 이에 비해 DPPH 라디칼 소거능은 열수추출물에 비해 에탄올 추출물(89.0%), 부탄올 추출물(88.9%)이 유의적으로 높은 값을 나타내었다. MIC 측정 결과, 적 백하수오 에탄올 추출물만이 0.8 mg/mL로 S. aureus와 P. acne에 대해서 항균효과를 나타냈다. 디스크 확산법 측정 결과, 모든 유기용매 추출물이 5 mg/disc 농도부터 생육저해환을 형성하였으며, 농도가 증가함에 따라 생육저해환의 크기도 증가하였다. 적하수오 및 백하수오 에탄올 추출물의 조사균주에 대한 생육저해완의 직경은 10 mg/mL 농도에서 각각 8.9 및 9.2 mm를 나타내, 추출물 중 가장 우수한 항균활성을 나타냈다. 본 연구는 하수오 추출물이 항산화 효과 뿐만 아니라 피부 건강과 관련된 세균에 대해 항균활성을 보여줌으로써, 하수오 추출물이 피부 건강을 위한 천연 유래의 기능성 화장품 소재로도 활용될 수 있는 잠재적 가능성을 제시하였다.

The objective of this research was to investigate the antioxidant and antimicrobial activities of Jeok Hasuo (Polygonum multiflorum Thunb., PM) and Baek Hasuo (Cynanchi wilfordii Radix, CW) root extracts. Total phenolic contents of PM and CW root extracts were determined and the antioxidant activities of the root extracts were determined by scavenging activity of diphenylpicrylhydrazyl (DPPH) radicals. The antimicrobial activities against Staphylococcus aureus and Propionibacterium acnes were determined and expressed as the minimum inhibitory concentration (MIC). The disc diffusion method was also used to determine the zone of inhibition. The butanol extracts of PM and CW roots exhibited greater total phenolic contents (1,212.6 and 1,454.5 mg/g GAE, respectively) than those of ethanol and water extracts. The ethanol (89.0%) and butanol extracts (88.9%) of PM exhibited significantly greater DPPH radical scavenging activities than that of water extracts (73.1%) (p<0.05). Only ethanol extract exhibited an MIC of 0.8 mg/mL against both bacteria. Zones of inhibition started to form when the concentration of extract was greater than 5 mg/disc. The diameters of the zone of inhibition of PM and CW were measured to be 8.9 and 9.2 mm against S. aureus and P. acnes, respectively, exhibiting the greatest antimicrobial activities among the extracts. This research demonstrated that the PM and CW root extracts possessed not only antioxidant activity but also strong antimicrobial activity against skin-related bacteria.

키워드

참고문헌

  1. Lee BJ, Lee KJ (2015) Discrimination and proper use of Polygoni multiflori radix, Cynanchi wilfordii radix, and Cynanchi auriculati radix in Korea : A descriptive review. Roy Soc Med Int Cong, 2015, 1-7
  2. Choi HS, Zhu M, Kim CS, Lee JH (2003) Studies of name and herbal origins of Ha-Soo-Oh. Kor J Orient Med, 9, 81-89
  3. Ministry of Food and Drug Safety (2015) The Korean herbal pharmacopoeia XI. Ministry of Food and Drug Safety, Osong, Korea
  4. Kim MJ, Kim IJ, Choi SY, Han DH, Kim YH, Lim SC, Kim TJ, Nam SY, Song BH, Oh BU, Park CG (2014) Comparison of Cynanchum wilfordii, C. auriculatum, Metaplexis japonica and Polygonum multiflorum by morphological characters. Korean J Medicinal Crop Sci, 22, 113-120 https://doi.org/10.7783/KJMCS.2014.22.2.113
  5. Seong ES, Hwang IS, Choi JH, Lee JG, Yoo JH, Ahn YS, Park CB, Yu CY (2015) Enhanced biomass and biological activity of 'Hasuo' (Polygonum multiflorum Thunberg) grown under LED light. Aust J Crop Sci, 9, 168-174
  6. Na YJ, Lee JH (2014) Physicochemical and antioxidant properities of Yanggaeng with Cynanchi wilfordii Radix Powder, J Korean Soc Food Sci Nutr, 43, 1954-1958 https://doi.org/10.3746/jkfn.2014.43.12.1954
  7. Seo H, Seo GY, Ko SZ, Park YH (2011) Inhibitory effects of ethanol extracts from Polygoni multiflori radix and Cynanchi wilfordii radix on melanogenesis in melanoma cells. J Korean Soc Food Sci Nutr, 40, 1086-1091 https://doi.org/10.3746/jkfn.2011.40.8.1086
  8. Furukawa T, Eshima A, Kouya M, Takio S, Takano H, Ono K (2002) Coordinate expression of genes involved in catechin biosynthesis in Polygonum hydropiper cells. Plant Cell Rep, 21, 385-389 https://doi.org/10.1007/s00299-002-0535-z
  9. Lo KM, Cheung PCK (2005) Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var. alba. Food Chem, 89, 533-539 https://doi.org/10.1016/j.foodchem.2004.03.006
  10. AOAC (1996) Official Methods of Analysis. 15th ed, Association of Official Analysis Chemist, Washing DC, p 210-210
  11. Mak, YW, Chuah LO, Ahmad R, Bhat R (2013) Antioxidant and antibacterial activities of hibiscus (Hibiscus rosa-sinensis L.) and Cassia (Senna bicapsularis L.) flower extracts. J King Saud Unive-Sci, 25, 275-282 https://doi.org/10.1016/j.jksus.2012.12.003
  12. Branen J, Davidson PM (2000) Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: The effect of EDTA. Lett Appl Microbiol, 30, 233-237 https://doi.org/10.1046/j.1472-765x.2000.00711.x
  13. Kwon NS (2015) Effectiveness of Polygonum multiflorum extracts as whitening, antibacterial, and antiseptic agents. MS Thesis, Daegu Hanny University, Korea, p 21-23
  14. Park HJ, Zhang N, Park DK (2011) Topical application of Polygonum multiflorum extract induces hair growth of resting hair follicles through upregulating Shh and $\beta$-catenin expression in C57BL/6 mice. J Ethnopharmacol, 135, 369-375 https://doi.org/10.1016/j.jep.2011.03.028
  15. Wong CC, Li HB, Cheng KW, Chen F (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem, 97, 705-711 https://doi.org/10.1016/j.foodchem.2005.05.049
  16. Chen Y, Wang M, Rosen RT, Ho CT (1999) 2,2-Diphenyl-1-picrylhydrazyl Radical-Scavenging Active Components from Polygonum multiflorum Thunb. J Agric Food Chem, 47, 2226-2228 https://doi.org/10.1021/jf990092f
  17. Hwang KE (2014) Combined effect of Ganghwa mugwort and ascorbid acid on shelf-life of meat products. MS Thesis, Konkuk University, Korea, p 38-45
  18. Lee SV, Choi KH, Choi YW, Hong JW, Baek JU, Choi BT, Shin HK (2014) Hexane extracts of Polygonum multiflorum improve tissue and functional outcome following focal cerebral ischemia in mice. Mol Med Rep, 9, 1415-1421 https://doi.org/10.3892/mmr.2014.1943
  19. Baydar NG, Sagdic O, Ozkan G, Cetin S (2006) Determination of antibacterial effects and total phenolic contents of grape (Vitis vinifera L.) seed extracts. Int J Food Sci Technol, 41,799-804 https://doi.org/10.1111/j.1365-2621.2005.01095.x
  20. Hasan MF, Das R, Khan A, Mossain MS, Rahman M (2009) The determination of antibacterial and antifungal activities of Polygonum hydropiper (L.) root extract. Adv Biologic Res, 3, 53-56

피인용 문헌

  1. 신선육자환 에탄올 추출물의 항산화 활성 vol.32, pp.5, 2017, https://doi.org/10.6116/kjh.2017.32.5.19
  2. Comparison of Active Compounds for Each Supplement after Processing Methods of KIOM Polygonum multiflorum Thunberg Using Various Ingredient(s) vol.54, pp.5, 2016, https://doi.org/10.14397/jals.2020.54.5.37